14-3-3 zeta Antibody

Western Blot: 14-3-3 zeta Antibody [NBP2-24595] - Analysis in human brain lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain lysate and 4) rat brain lysate using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: 14-3-3 zeta Antibody [NBP2-24595] - Staining of 14-3-3 zeta in human brain tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.

Product Discontinued

14-3-3 zeta Antibody Summary

• Immunogen: A portion of amino acids 50-100 of human 14-3-3 zeta was used as the immunogen.
• Predicted Species: Rat (93%), Monkey (93%), Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: YWHAZ
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 3-5 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for 14-3-3 zeta Antibody

• 1433 zeta
• 14-3-3 zeta
• KCIP-1
• KCIP-1MGC126532,14-3-3-zeta
• MGC111427
• MGC138156
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, deltapolypeptide
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zetapolypeptide
• YWHAZ
• zeta polypeptide

Background

14-3-3 zeta is a protein that mediates signal transduction by binding to phosphoserine-containing proteins. The protein is widely expressed in various gray matter regions of the brain. Overexpression of the 14-3-3 zeta gene has been found to correlate with chemoresistance to anthracyclines and metastatis recurrence of human breast cancer. These effects are likely caused by an anti-apoptotic mechanism of 14-3-3 zeta during anthracycline treatment.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: YWHAZ