ABCB11 Antibody - BSA Free

Western Blot: ABCB11 Antibody [NBP1-31824] - Various tissue extracts (5 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BSEP antibody [N3C1], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunocytochemistry/Immunofluorescence: ABCB11 Antibody [NBP1-31824] - Sample: A431 cells were fixed in iced-cold MeOH for 5 min. Green: ABCB11 protein stained by BSEP antibody, Internal diluted at 1:500. Blue: Hoechst 33343 staining.

Immunohistochemistry-Paraffin: ABCB11 Antibody [NBP1-31824] - AGS xenograft, using BSEP antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: ABCB11 Antibody [NBP1-31824] - ABCB11 antibody [N3C1], Internal detects ABCB11 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.ABCB11 stained by ABCB11 antibody [N3C1], Internal (NBP1-31824) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: ABCB11 Antibody [NBP1-31824] -Boiled and unboiled mouse liver extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BSEP antibody [N3C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ABCB11 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human ABCB11. The exact sequence is proprietary.
• Localization: Membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ABCB11
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 146 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%), Canine (85%), Feline (89%), Rabbit (84%), Bovine (89%), Guinea pig (82%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Protein A purified

Alternate Names for ABCB11 Antibody - BSA Free

• ABC member 16, MDR/TAP subfamily
• ABC16
• ATP-binding cassette sub-family B member 11
• ATP-binding cassette, sub-family B (MDR/TAP), member 11
• bile salt export pump
• BSEP
• BSEPPFIC2
• EC 3.6.3
• EC 3.6.3.44
• PFIC-2
• PGY4
• progressive familial intrahepatic cholestasis 2
• sister p-glycoprotein
• SPGPBRIC2

Background

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is the major canalicular bile salt export pump in man. Mutations in this gene cause a form of progressive familial intrahepatic cholestases which are a group of inherited disorders with severe cholestatic liver disease from early infancy. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ABCB11