ABCG8 Antibody - BSA Free

Western Blot: ABCG8 Antibody [NB400-117] - Total protein from Mouse small intestine was separated on a 7.5% gel by SDS-PAGE and transferred to PVDF membrane. The membrane was then probed with anti-ABCG8 antibody at 2 ug/ml and detected with an anti-rabbit HRP conjugated secondary antibody using chemiluminescence.

Immunocytochemistry/Immunofluorescence: ABCG8 Antibody [NB400-117] - ABCG8 antibody was tested in HEK293 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ABCG8 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide from the N-terminal region of human ABCG8 protein. [UniProt# Q9H221]
• Localization: Integral membrane protein; multi-pass membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ABCG8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:250
  • Western Blot 1:250-1:500
• Application Notes: This ABCG8 antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot where a doublet is seen at ~67kDa and ~72kDa. Longer exposure times are needed to visualize the wild type ABCG8. For mouse, 100 ug protein (B6/129AKRFVB liver homogenate) loaded with antibody diluted at 1:500. In ICC/IF puncate membrane localization with some weak nuclear reactivity was observed in HEK293 cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  ABCG8 Peptide (NB400-117PEP)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ABCG8 Antibody - BSA Free

• ATP-binding cassette sub-family G member 8
• ATP-binding cassette, sub-family G (WHITE), member 8 (sterolin 2)
• ATP-binding cassette, sub-family G (WHITE), member 8
• GBD4ATP-binding cassette, subfamily G, member 8
• MGC142217
• sterolin 2
• sterolin-2
• STSL

Background

ATP-binding cassette (ABC) transporter genes are involved in the regulation of the amount of dietary cholesterol retained in the body. ABCG8, expressed at high levels in the liver and intestine, normally cooperates with ABCG5 to limit intestinal absorption and promote biliary excretion of sterols. The mutated form of this transporter can lead to sterol accumulation and atherosclerosis or sitosterolemia, a rare autosomal recessive disorder, characterized by hyperabsorption of sterols and the inability to excrete sterols into bile.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ABCG8 Antibody (NB400-117)(7)

Publications using NB400-117

• Sachdev V, Leopold C, Bauer R et al. Novel role of a triglyceride-synthesizing enzyme: DGAT1 at the crossroad between triglyceride and cholesterol metabolism. Molecular and Cell Biology of Lipids. 2016-06-23 [PMID: 27344248] (WB)
  
  Details:
  This citation used the Alexa Fluor 488 version of this antibody.
• Heverin M, Ali Z, Olin M et al. On the regulatory importance of 27-hydroxycholesterol in mouse liver J. Steroid Biochem. Mol. Biol. 2016-02-03 [PMID: 26851362] (WB, Mouse)
• Huang Y, Wang J, Quan G et al. Lactobacillus acidophilus ATCC 4356 prevents atherosclerosis via inhibition of intestinal cholesterol absorption in Apolipoprotein E-knockout mice. Appl. Environ. Microbiol. 2014-09-26 [PMID: 25261526] (WB, Mouse)
  
  Details:
  ABCG5 antibody used for WB on total proteins isolated from small intestines of ApoE?/? mice treated with or without Lactobacillus acidophilus / ATCC 4356 (FIG 7C).
• Bujold K, Mellal K, Zoccal KF et al. EP 80317, a CD36 selective ligand, promotes reverse cholesterol transport in apolipoprotein E-deficient mice. Atherosclerosis 2013-08-01 [PMID: 23880196] (WB, Mouse)
• Levy E, Ouadda AB, Spahis S et al. PCSK9 plays a significant role in cholesterol homeostasis and lipid transport in intestinal epithelial cells. Atherosclerosis 2013-02-04 [PMID: 23422832] (WB, Human)
  
  Details:
  Using the HRP conjugated version of NB400-128, catalog number NB400-128H.
• Grenier E, Garofalo C, Delvin E, Levy E. Modulatory Role of PYY in Transport and Metabolism of Cholesterol in Intestinal Epithelial Cells plos One 2012-01-01 [PMID: 22844422] (WB, Human)
• Mathur, SN, Watt, KR, Field, FJ. Regulation of intestinal NPC1L1 expression by dietary fish oil and docosahexaenoic acid. J Lipid Res;48(2):395-404. 2007-02-01 [PMID: 17114806]

Bioinformatics

• Gene Symbol: ABCG8