ACADVL Antibody

Western Blot: ACADVL Antibody [NBP1-52016] - (2ug/ml) Analysis of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ACADVL Antibody Summary

• Immunogen: Peptide with sequence DKSDSHPSDALTRK-C corresponding to N-Terminus (near) according to NP_000009.1, NP_001029031.1.
• Epitope: DKSDSHPSDALTRK-C
• Specificity: This antibody is expected to recognize both reported isoforms (NP_000009.1; NP_001029031.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ACADVL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 68 kDa band observed in human heart lysates (calculated MW of 68.1 kDa band according to NP_001029031.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ACADVL Antibody

• ACAD6
• acyl-CoA dehydrogenase, very long chain
• acyl-Coenzyme A dehydrogenase, very long chain
• EC 1.3.99
• EC 1.3.99.-
• LCACD
• very long-chain specific acyl-CoA dehydrogenase, mitochondrial
• VLCAD

Background

The protein encoded by this gene is targeted to the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway. This acyl-Coenzyme A dehydrogenase is specific to long-chain and very-long-chain fatty acids. A deficiency in this gene product reduces myocardial fatty acid beta-oxidation and is associated with cardiomyopathy. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ACADVL Antibody (NBP1-52016)(1)

Publication using NBP1-52016

• Gobin-Limballe S, Djouadi F, Aubey F et al. Geneticbasis for correction of very-long-chain acyl-coenzyme A dehydrogenase deficiency by bezafibrate in patient fibroblasts: toward a genotype-based therapy. Am J Hum Genet 2007-12-01 [PMID: 17999356]

Bioinformatics

• Gene Symbol: ACADVL
• Entrez:
  • 37(Human)
• Uniprot:
  • NP_000009.1(Human)