Actin Antibody (AC-40)

Product Discontinued

Actin Antibody (AC-40) Summary

• Immunogen: Synthetic actin C-terminal peptide Ser-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe, attached to a Multiple Antigen Peptide (MAP) backbone.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACTA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 4 ug/ml
  • Western Blot 2 ug/ml

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 1.2% sodium acetate, and 2mg BSA
• Preservative: 0.01 mg Sodium Azide
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with 0.1 ml distilled water.

Alternate Names for Actin Antibody (AC-40)

• ACTA
• Actin
• actin, alpha 1, skeletal muscle
• alpha skeletal muscle actin
• alpha skeletal muscle
• alpha-actin-1
• ASMA
• CFTD
• CFTDM
• MPFD
• NEM1
• NEM2
• NEM3

Background

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Actin Antibody (NBP1-41294)(2)

Publications using NBP1-41294

• Peters HL, Yan Y, Solheim JC. APLP2 regulates the expression of MHC class I molecules on irradiated Ewing's sarcoma cells. Oncoimmunology 2013-10-01 [PMID: 24353913]
• Peters HL, Yan Y, Nordgren TM et al. Amyloid precursor-like protein 2 suppresses irradiation-induced apoptosis in Ewing sarcoma cells and is elevated in immune-evasive Ewing sarcoma cells. Cancer Biol Ther 2013-08-01 [PMID: 23792571] (Human)

Review for Actin Antibody (NBP1-41294) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Ronald Miller WB Human 05/29/2014

Summary

• Application: Western Blot
• Sample Tested: Young and senescent cells
• Species: Human

FAQs for Actin Antibody (NBP1-41294). (Showing 1 - 1 of 1 FAQs).

1. I am interested in buying an Actin antibody to use in Western blot as a loading control. We would like to use it on rat heart samples. Can you make a recommendation?
   • I would recommend the Actin Antibody (mAbGEa) catalog # NB100-74340 . This antibody works great as a loading control in Western blot and has been validated for use with rat samples.

Bioinformatics

• Gene Symbol: ACTA1
• Entrez:
  • 58(Human)
• Uniprot:
  • P68133(Human)