Recombinant Human LIGHT/TNFSF14 Protein

Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI) stimulates cell proliferation in HUVEC human umbilical vein endothelial cells. The ED50 is 1-4 ng/mL.

1 µg/lane of Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 25 kDa.

• Reactivity: Hu
• Applications: Bioactivity

Recombinant Human LIGHT/TNFSF14 Protein Summary

• Details of Functionality: Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED₅₀ for this effect is 0.3-3 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human LIGHT/TNFSF14 protein
  

  Met	10-His tag	GGGSGGGSGGGSIEGR	Human Light (Asp74-Val240) Accession # O43557
  N-terminus			C-terminus

• Accession #: O43557
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: TNFSF14
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity2
• Theoretical MW: 20.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 25 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LIGHT/TNFSF14 Protein

• CD258 antigen
• CD258
• delta transmembrane LIGHT
• Herpes virus entry mediator ligand
• Herpesvirus entry mediator ligand
• HVEM-L
• HVEMLherpesvirus entry mediator-ligand
• ligand for herpesvirus entry mediator
• LIGHT
• LIGHTherpesvirus entry mediator A
• LTg
• TNFSF14
• TR2
• tumor necrosis factor (ligand) superfamily, member 14
• tumor necrosis factor ligand superfamily member 14
• tumor necrosis factor receptor-like 2
• tumor necrosis factor superfamily member LIGHT

Background

Human LIGHT is a type II membrane protein that is a member of the TNF superfamily. LIGHT is an acronym which stands for "is homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes". LIGHT has also been called HVEM-L and LT-gamma . Under the new TNF nomenclature, it is called TNFSF14. LIGHT is a 240 amino acid (aa) protein that contains a 37 aa cytoplasmic domain, a 22 aa transmembrane region, and a 181 aa extracellular domain. Similar to other TNF ligand family members, LIGHT is predicted to assemble as a homotrimer. LIGHT is produced by activated T cells and was first identified by its ability to compete with HSV glycoprotein D for HVEM binding. LIGHT has also been shown to bind to the lymphotoxin beta receptor (LT beta R) and the decoy receptor (DcR3/TR6). LIGHT overexpression in tumor cells induces apoptosis, which can be enhanced by IFN-gamma . The full roles of LIGHT remain to be elucidated.

1. Mauri, D.N. et al. (1998) Immunity 8:21.
2. Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
3. Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548.
4. Yu, K-Y. et al. (1999) J. Biol. Chem. 274:13733.

Publications for LIGHT/TNFSF14 (664-LI)(21)

Publications using 664-LI

• Di Modugno, F;Di Carlo, A;Spada, S;Palermo, B;D'Ambrosio, L;D'Andrea, D;Morello, G;Belmonte, B;Sperduti, I;Balzano, V;Gallo, E;Melchionna, R;Panetta, M;Campo, G;De Nicola, F;Goeman, F;Antoniani, B;Carpano, S;Frigè, G;Warren, S;Gallina, F;Lambrechts, D;Xiong, J;Vincent, BG;Wheeler, N;Bortone, DS;Cappuzzo, F;Facciolo, F;Tripodo, C;Visca, P;Nisticò, P; Tumoral and stromal hMENA isoforms impact tertiary lymphoid structure localization in lung cancer and predict immune checkpoint blockade response in patients with cancer EBioMedicine 2024-02-09 [PMID: 38340557] (Bioassay, Human)
• Heo, SK;Yu, HM;Kim, DK;Seo, HJ;Shin, Y;Kim, SA;Kim, M;Kim, Y;Lee, YJ;Noh, EK;Jo, JC; LIGHT (TNFSF14) promotes the differentiation of human bone marrow-derived mesenchymal stem cells into functional hepatocyte-like cells PloS one 2023-08-08 [PMID: 37552689] (Bioassay, Human)
• De Martin, A;Stanossek, Y;L�tge, M;Cadosch, N;Onder, L;Cheng, HW;Brandstadter, JD;Maillard, I;Stoeckli, SJ;Pikor, NB;Ludewig, B; PI16+ reticular cells in human palatine tonsils govern T cell activity in distinct subepithelial niches Nature immunology 2023-05-18 [PMID: 37202490] (Bioassay, Human)
• SK Heo, Y Choi, YK Jeong, LJ Ju, HM Yu, DK Kim, HJ Seo, YJ Lee, J Cheon, S Koh, YJ Min, EK Noh, JC Jo LIGHT (TNFSF14) enhances osteogenesis of human bone marrow-derived mesenchymal stem cells PLoS ONE, 2021-02-19;16(2):e0247368. 2021-02-19 [PMID: 33606781] (Bioassay, Human)
• Jang H, Park S, Kim J, Kim J, Kim S, Cho S, Park S, Park B, Kim S, Kim J The Tumor Suppressor, p53, Negatively Regulates Non-Canonical NF-kappaB Signaling through miRNAInduced Silencing of NF-kappaB-Inducing Kinase. Mol Cells, 2020-01-31;43(1):23-33. 2020-01-31 [PMID: 31870133] (Bioassay, Human)
• S An, I Raju, B Surenkhuu, JE Kwon, S Gulati, M Karaman, A Pradeep, S Sinha, C Mun, S Jain Neutrophil extracellular traps (NETs) contribute to pathological changes of ocular graft-vs.-host disease (oGVHD) dry eye: Implications for novel biomarkers and therapeutic strategies Ocul Surf, 2019-04-06;0(0):. 2019-04-06 [PMID: 30965123] (Bioassay, Human)
• KH Kim, H Choi, HJ Kim, TR Lee TNFSF14 inhibits melanogenesis via NF-kB signaling in melanocytes Cytokine, 2018-05-03;110(0):126-130. 2018-05-03 [PMID: 29730385] (Bioassay, Human)
• R da Silva A, AK Mehta, L Madge, J Tocker, M Croft TNFSF14 (LIGHT) Exhibits Inflammatory Activities in Lung Fibroblasts Complementary to IL-13 and TGF-β Front Immunol, 2018-03-19;9(0):576. 2018-03-19 [PMID: 29616048] (Cell Culture, Human)
• AW Clarke, L Poulton, D Shim, D Mabon, D Butt, M Pollard, V Pande, J Husten, J Lyons, C Tian, AG Doyle An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease MAbs, 2018-03-05;0(0):1-43. 2018-03-05 [PMID: 29436901] (Surface Plasmon Resonance (SPR, Human)
• S Abadpour, B Halvorsen, A Sahraoui, O Korsgren, P Aukrust, H Scholz Interleukin-22 reverses human islet dysfunction and apoptosis triggered by hyperglycemia and LIGHT J. Mol. Endocrinol., 2018-01-12;0(0):. 2018-01-12 [PMID: 29330151] (Bioassay, Human)
• X Yuan, Y Gu, X Lai, Q Gu LIGHT is increased in patients with coronary disease and regulates inflammatory response and lipid metabolism in oxLDL-induced THP-1 macrophages Biochem. Biophys. Res. Commun., 2017-06-19;0(0):. 2017-06-19 [PMID: 28642135] (Bioassay, Human)
• A TNFRSF14-Fc?RI-mast cell pathway contributes to development of multiple features of asthma pathology in mice Nat Commun, 2016-12-16;7(0):13696. 2016-12-16 [PMID: 27982078] (Bioassay, Human)
• LIGHT (TNFSF14) Increases the Survival and Proliferation of Human Bone Marrow-Derived Mesenchymal Stem Cells PLoS ONE, 2016-11-11;11(11):e0166589. 2016-11-11 [PMID: 27835685] (Bioassay, Human)
• da Silva Antunes R, Madge L, Soroosh P, Tocker J, Croft M The TNF Family Molecules LIGHT and Lymphotoxin alphabeta Induce a Distinct Steroid-Resistant Inflammatory Phenotype in Human Lung Epithelial Cells. J Immunol, 2015-07-24;195(5):2429-41. 2015-07-24 [PMID: 26209626] (Bioassay, Human)
• Herro R, Antunes R, Aguilera A, Tamada K, Croft M The Tumor Necrosis Factor Superfamily Molecule LIGHT Promotes Keratinocyte Activity and Skin Fibrosis. J Invest Dermatol, 2015-03-19;135(8):2109-18. 2015-03-19 [PMID: 25789702] (Bioassay, Human, Mouse)
• Mikami Y, Matsuzaki H, Horie M, Noguchi S, Jo T, Narumoto O, Kohyama T, Takizawa H, Nagase T, Yamauchi Y Lymphotoxin beta receptor signaling induces IL-8 production in human bronchial epithelial cells. PLoS ONE, 2014-12-11;9(12):e114791. 2014-12-11 [PMID: 25501580] (Bioassay, Human)
• Jin HR, Jin X, Lee JJ Zinc-finger protein 91 plays a key role in LIGHT-induced activation of non-canonical NF-kappaB pathway. Biochem. Biophys. Res. Commun., 2010-09-06;400(4):581-6. 2010-09-06 [PMID: 20804734] (Bioassay, Human)
• Sandberg WJ, Halvorsen B, Yndestad A, Smith C, Otterdal K, Brosstad FR, Froland SS, Olofsson PS, Damas JK, Gullestad L, Hansson GK, Oie E, Aukrust P Inflammatory interaction between LIGHT and proteinase-activated receptor-2 in endothelial cells: potential role in atherogenesis. Circ. Res., 2008-11-20;104(1):60-8. 2008-11-20 [PMID: 19023130] (Bioassay, Human)
• Kang YM, Kim SY, Kang JH LIGHT up-regulated on B lymphocytes and monocytes in rheumatoid arthritis mediates cellular adhesion and metalloproteinase production by synoviocytes. Arthritis Rheum., 2007-04-01;56(4):1106-17. 2007-04-01 [PMID: 17393389] (Bioassay, Human)
• Otterdal K, Smith C, Oie E, Pedersen TM, Yndestad A, Stang E, Endresen K, Solum NO, Aukrust P, Damas JK Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes. Blood, 2006-08-01;108(3):928-35. 2006-08-01 [PMID: 16861346] (Bioassay, Human)
• Edwards JR, Sun SG, Locklin R, Shipman CM, Adamopoulos IE, Athanasou NA, Sabokbar A LIGHT (TNFSF14), a novel mediator of bone resorption, is elevated in rheumatoid arthritis. Arthritis Rheum., 2006-05-01;54(5):1451-62. 2006-05-01 [PMID: 16649193] (Bioassay, Human)

Bioinformatics

• Gene Symbol: TNFSF14
• Uniprot:
  • O43557()