Actin Antibody (mAbGEa) - BSA Free

Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Total protein from HeLa, 3T3, PC12 and Bovine normal tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Actin in 5% blocking buffer and detected with an anti-mouse IgM secondary antibody using chemiluminescence.

Immunocytochemistry/Immunofluorescence: Actin Antibody (mAbGEa) [NB100-74340] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Actin (mAbGEa) at 5 ug/mL overnight at 4C and detected with an anti-Mouse IgM DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Simple Western: Actin Antibody (mAbGEa) [NB100-74340] - Image shows a specific band for Actin in 0.1 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunohistochemistry-Paraffin: Actin Antibody (mAbGEa) [NB100-74340] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.

Flow Cytometry: Actin Antibody (mAbGEa) [NB100-74340] - Analysis of HeLa cells using mouse Actin antibody (Orange) and Isotype control Antibody (Blue).

Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Increased relative abundance of Park7 in Lp(a) mice. Western blots were used to validate the comparative proteomic analysis between the Lp(a) and wildtype mice for the Park7 protein. Pooled liver protein extracts (n = 4 livers per pool) were separated by SDS PAGE in multiple replicates (n = 7) and transferred onto nitrocellulose membrane. Membranes were probed with an anti-Park7 antibody using an anti-actin antibody as a loading control. Liver protein extracts were the same as those used for Figure 4(a) as well as fresh liver protein extracts from new mice of the same age, sex, and genotype. Image collected and cropped by CiteAb from the following publication (null), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Actin Antibody (mAbGEa) [NB100-74340] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Actin Antibody (mAbGEa) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow (Intracellular): Actin Antibody (mAbGEa) [NB100-74340] - An intracellular stain was performed on A549 cells with NB100-74340 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse IgM Alexa Fluor 488-conjugated secondary antibody.

Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Analysis of Actin expression in 2) HeLa, 3) NTERA-2, 4) A431, 5) HepG2, 6) MCF7, 7) NIH-3T3, 8) PC-12 and 9) COS-7 whole cell lysates.

Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Analysis of Jurkat and CHO cell lysates using actin antibody [NB100-74340] at 1:100.

Immunocytochemistry/Immunofluorescence: Actin Antibody (mAbGEa) [NB100-74340] - Actin was detected in NIH-3T3 cells fixed with methanol using mouse anti-mouse beta-Actin monoclonal antibody (NB100-74340) at 1:1800. Cells were stained using Northern Lights 557 conjugated anti-mouse secondary antibody (NL007) and counterstained with DAPI.

Immunocytochemistry/Immunofluorescence: Actin Antibody (mAbGEa) [NB100-74340] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Actin (mAbGEa) at 2 ug/mL overnight at 4C and detected with an anti-mouse IgM DyLight 488 (Green) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: Actin Antibody (mAbGEa) [NB100-74340] - Analysis of Actin on human breast cancer tissue using DAB with hematoxylin counterstain.

Immunohistochemistry-Paraffin: Actin Antibody (mAbGEa) [NB100-74340] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.

Immunohistochemistry-Paraffin: Actin Antibody (mAbGEa) [NB100-74340] - Both normal and cancer biopsies of deparaffinized Human skeletal muscle tissues.

Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Lp(a) upregulates GPx1 & Prdx6 expression in human HepG2 cells. HepG2 cells were treated with 5 μg/mL of Lp(a) or LDL for 6 hours at 37°C. Western blots of cell lysates were performed with an anti-Gpx1 antibody (a), an anti-Prdx6 antibody (b), an anti-Sod1 antibody (c), & an anti-Park7 antibody (d) using an anti-actin antibody as a loading control. Representative blots are shown. Protein levels were normalized against actin & expressed relative to that of untreated cells. Results are expressed as mean ± SEM for pooled triplicate incubations run in quadruplicate. ∗P<0.05, relative to untreated HepG2 cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30596094), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - DenA deneddylase activity.(A) Recombinant human DEN1 & fungal DenA deneddylate a human CUL1-Nedd8 substrate in vitro. SDS-PAGE & subsequent western analysis show cleavage of the substrate (∼60 kDa) producing the C-terminal CUL1 (∼50 kDa) as outlined in experimental procedures. (B) Deneddylation test in a heterologous yeast system. A. nidulans DenA can remove Rub1 from CulD in heterologous expression experiments in S. cerevisiae. DenA expressed as native protein or C-terminally fused w/ a V5/His6 epitope tag. Both variants driven by the inducible GAL1 promoter. CulD, N-terminal-fused w/ the LexA activation domain, expressed under control of the constitutive ADH promoter. A. nidulans proteins expressed in S. cerevisiae wild type & delta csn5 background. Western analysis w/ antibodies against Rub1 ( alpha -Rub1), the LexA epitope ( alpha -LexA) & the V5 epitope ( alpha -V5) performed. Detection w/ alpha -Rub1 generated two additional signals upon culD expression, representing LexA-CulD & a second CulD pool where LexA unspecifically cleaved off. Both signals disappeared upon co-expression of DenA indicating deneddylation activity (red arrows). The slower migrating signal of alpha -LexA western experiments corresponded to Rub1 modified LexA-CulD. This signal absent when DenA co-expressed. Detection of the V5 tag applied to monitor DenA expression. The neddylated yeast cullin migrating at around 100 kDa not affected by DenA activity. (C) Deneddylation of fungal CulA by CSN & DenA. Whole cell lysates of A. nidulans wild type, delta csnE & delta denA probed w/ alpha -CulA. The ratio of neddylated CulA (CulA*N8; ∼106 kDa*) to non-neddylated CulA (∼96 kDa**) calculated from three independent experiments. Membranes reprobed w/ alpha -Actin ( alpha -ACT) for normalization. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1003275), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Fibrillar adhesions & FN fibrils are dynamic structures governed by Rab21 & PPFIA1.(a) Living siCTL & siRAB21 ECs incubated w/ IST9 mAb for 30 min, fixed, acid washed & stained. RAB21 silencing impairs ED-A FN endocytosis as revealed by decrease of IST9 punctae co-localizing w/ endosome marker EEA1. Right panels are magnifications of boxed areas in left panels. Data are mean value±s.e.m., n=70 cells per condition pooled from two independent experiments. ***P<0.001. (b) Left panel, WB analysis of PPFIA1, RAB21 & actin on total lysates of siCTL, siPPFIA1 & siPPFIA1+siRAB21 ECs. Right panel, WB analysis of insoluble matrix fraction of ECs extracted w/ DOC buffer. PPFIA1 silencing dramatically reduces amount of DOC-insoluble fraction of endogenous ED-A FN. Of note, simultaneous silencing of Rab21 GTPase (siPPFIA1+siRAB21), which drives integrin endocytosis, rescues defective incorporation of endogenous ED-A FN in DOC-insoluble fraction of siPPFIA1 ECs. (c) Confocal microscopy analysis of patterning of endogenous cellular ED-A FN (green) in fixed confluent ECs. Before fixation, living ECs incubated for 20 min w/ exogenous SNAKA51 (red). ED-A FN polymerizes into a fibrillar network in siCTL, but not in siPPFIA1 ECs. Simultaneous silencing of Rab21 GTPase (siPPFIA1+siRAB21) fully restores ED-A FN polymerization in siPPFIA1 ECs. SNAKA51+ active alpha 5 beta 1 integrin localizes in fibrillar adhesion in siCTL, but not in siPPFIA1 ECs. Notably, simultaneous Rab21 (siPPFIA1+siRAB21) silencing promotes localization of SNAKA51 in fibrillar adhesion of siPPFIA1 ECs. Data are mean±s.e.m., n=20 cells per condition pooled from two independent experiments. Scale bar, 20 μm (a,c, right), 50μm (c, left). ***P<0.001; Student's t-test. Image collected & cropped by CiteAb from following publication (//www.nature.com/articles/ncomms13546), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Effect of the four most common herbal formulas & single herbs on the phosphorylation of myosin light chain (MLC) protein.Briefly, A10 cells were treated with herbal formulas (A) or single herbs (B). Y27632 (Y10; 10 μM) & calyculin A (A50; 50 μg/ml) were used as negative & positive controls. Western blot analysis & staining with anti-phospho-MLC, anti-total-MLC, & anti-beta actin antibodies was then performed. Phospho-MLC, total-MLC, & beta actin were all obtained with their appropriate protein size bands. The relative Phospho-MLC intensity (%) was expressed as [(Phospho-MLC/total-MLC)drug treated/ (Phospho-MLC/total-MLC)cell only x 100%]. The Mean±SEM values for at least three independent experiments along with the representative western blot were performed. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0145109), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - CSN targets DEN1/DenA for degradation in fungal & human cells.(A) Quantitative analysis of repeated western blot experiments displayed the differences in DenA abundance in fungal wild type & delta csnG cells. DenA levels of the different asexual developmental time points are shown relative to the vegetative (veg.) control for each strain. Anti-Actin was applied as loading control (statistics: 2-way ANOVA; n = 3; *p<0,05, **p<0,01). (B) Xpress-DEN1 was overexpressed in siGFP & siCSN1 human cells & steady state Xpress-DEN1 levels were estimated by western analysis with the alpha -Xpress antibody. Xpress-CSN1 was overexpressed in siCSN1 cells & DEN1 & CSN8 were probed with appropriate antibodies. (C) Xpress-DEN1 was overexpressed in siGFP human cells & the proteasome inhibitor MG132 was added 6 h before cell lysis at a final concentration of 10 µM. Cyclohexamide (CHX) was added in a final concentration of 10 µg/ml (D) Xpress-DEN1 was co-expressed in HeLa cells together with Xpress-CSN1wt, Xpress-CSN1(1–221) or Xpress-CSN1(222–527) in the absence or in the presence of MG132 (right panel), which was added 6 h before cell lysis. Cells were lyzed 24 h after co-transfection & lysates were analyzed by western blot using the alpha -DEN1 antibody (0 = only Xpress-DEN1). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1003275), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Analysis of Ia production by engineered S. cerevisiae.(A) Analysis of the synthesis of Ia in S. cerevisiae strains, producing actin-R177K, E270D & E270Q. Yeast strains, producing actin-R177K, E270D or E270Q & transformed with the Ia-containing plasmid (Ia) or the vector alone (Vector), were cultivated in SGal for 20 h at 30°C. Cells were broken by glass beads treatment & analyzed by 32P-ADP-ribosylation in the presence of additionally added purified wild type yeast actin (1 μg). Labeled bands represent modified yeast actin & confirm intracellular production of functionally active Ia by the S. cerevisiae strains. (B) Production of Ia by the wild type S. cerevisiae strain. Wild type yeast strains harboring the Ia-containing plasmid (Ia) or the control vector (vector) were cultivated in glucose-containing liquid medium until OD595 = 0.5. Afterwards, glucose was replaced by galactose & cultivation continued for 9 h at 30°C. Cells were lysed & the resulting extract preparations were ADP-ribosylated in the presence of Ia (+ Ia), TccC3 toxin of P. luminescens [42] (+ TccC3), purified muscle actin (+ alpha -actin) or tested in Western blotting with the anti-actin serum to show equal actin concentrations in the samples. (C, D) Mass spectrometry of actin variants. MALDI-TOF MS of wild type (C) & actin-R177K (D) protein variants isolated from S. cerevisiae. Spectra demonstrate disappearance of R177- & appearance of K177-containing peptide in mass analysis (substituted amino acid residue within identified peptides is shown in red). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0145708), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - alpha 5 beta 1 regulates ED-A FN secretion & polymerization.(a) Western blot analysis of lysates of ECs control (siCTL) & alpha 5 integrin subunit (siITGA5) silenced ECs. Cells were lysed 24 hours after the second siRNA oligofection & proteins were separated by SDS–PAGE & probed for alpha 5 integrin subunit or actin (for control purposes). (b) Confocal microscopy analysis of IST9 mAb-labelled endogenous ED-A FN (green) in confluent ECs. ED-A FN polymerizes into a fibrillar network in siCTL, but not in siITGA5 ECs in which it accumulates in the TGN46+ (red) TGN cisternae. The relative amount of fibrillar ED-A FN area was calculated in siCTL & siITGA5 ECs. Data are mean±s.e.m., n=20 cells per condition pooled from two independent experiments. ***P<0.001; Student's t-test. (c) Western blot analysis of soluble ED-A FN released by confluent ECs seeded on Transwell inserts. An equal percentage of apical & basolateral volumes of medium were collected after 72 h of culture, from different wells of siCTL or siITGA5 ECs. Equal amounts of exogenous rabbit IgG were added to samples (spike normalization) for loading control purposes. Quantification of the ratio between apical or basolateral amount of ED-A FN released by siCTL over siITGA5 ECs. alpha 5 integrin subunit silencing impairs basolateral, but not apical ED-A FN secretion. Data are mean±s.e.m., n=8 wells per condition pooled from four independent experiments. **P<0.01; Student's t-test. Scale bar, 50 μm (b). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms13546), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Effect of the four most common herbal formulas & single herbs on the phosphorylation of myosin light chain (MLC) protein.Briefly, A10 cells were treated with herbal formulas (A) or single herbs (B). Y27632 (Y10; 10 μM) & calyculin A (A50; 50 μg/ml) were used as negative & positive controls. Western blot analysis & staining with anti-phospho-MLC, anti-total-MLC, & anti-beta actin antibodies was then performed. Phospho-MLC, total-MLC, & beta actin were all obtained with their appropriate protein size bands. The relative Phospho-MLC intensity (%) was expressed as [(Phospho-MLC/total-MLC)drug treated/ (Phospho-MLC/total-MLC)cell only x 100%]. The Mean±SEM values for at least three independent experiments along with the representative western blot were performed. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0145109), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Bv, ChHa, Dr, Fu, Pl, Pr, Rb, Sh, Xp, Ye, Ze
• Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC
• Clone: mAbGEa
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Actin Antibody (mAbGEa) - BSA Free Summary

• Immunogen: Purified recombinant Arabidopsis Actin protein [UniProt# P0CJ46]
• Localization: Cytoplasm > cytoskeleton
• Specificity: Reacts with Actin 1, 2, 3, 4, 7, 8, 11 and 12.
• Isotype: IgM Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACTA1
• Purity: IgM purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Flow (Intracellular) 1 ug/ml
  • Flow Cytometry 1 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:20-1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Simple Western 1:25
  • Western Blot 1:100-1:1000
• Application Notes: In Western blot, a band is seen at ~45 kDa representing Actin. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. In IHC-P and ICC/IF, cytoplasmic staining is observed. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 0.1 mg/mL, separated by Size, antibody dilution of 1:25, apparent MW was 50 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Control:

  NIH 3T3 Whole Cell Lysate
  A-431 Whole Cell Lysate
  HeLa Whole Cell Lysate
  MCF-7 Whole Cell Lysate
  HepG2 Whole Cell Lysate
  NTERA-2 Whole Cell Lysate
  PC-12 Whole Cell Lysate

Reactivity Notes

Chinese hamster data from customer review. Sordaria macrospora reactivity reported in scientific literature (PMID: 24720701).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: IgM purified

Alternate Names for Actin Antibody (mAbGEa) - BSA Free

• ACTA
• Actin
• actin, alpha 1, skeletal muscle
• alpha skeletal muscle actin
• alpha skeletal muscle
• alpha-actin-1
• ASMA
• CFTD
• CFTDM
• MPFD
• NEM1
• NEM2
• NEM3

Background

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Actin Antibody (NB100-74340)(33)

Publications using NB100-74340

• Parra Bravo C, Krukowski K, Barker S et Al. Anti-acetylated-tau immunotherapy is neuroprotective in tauopathy and brain injury Mol Neurodegener 2024-06-24 [PMID: 38915105]
• Wang C, Zhang Y, Zhou B et al. Modifications of Two ESCRT-I Subunits with Distinct Ubiquitin Chains Regulate Plant Immunity bioRxiv 2023-08-16 (WB, Plant)
  
  Details:
  Arabidopsis thaliana
• Biehler C, Rothenberg K, Jetté A, et al. Functional plasticity of polarity proteins controls epithelial tissue architecture bioRxiv 2021-01-06 (WB, Drosophila)
• Redhu AK, Bhat JP Novel Proteomic changes in Yeast Mitochondria provide insights into mitochondrial functioning upon over-expression of human p53 bioRxiv 2019-08-24 (WB, Yeast)
• Biehler C, Rothenberg Ke, Jette A Et Al. Pak1 and PP2A antagonize aPKC function to support cortical tension induced by the Crumbs-Yurt complex eLife 2021-07-02 [PMID: 34212861] (WB)
• Redhu AK, Bhat JP Mitochondrial glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase abrogate p53 induced apoptosis in a yeast model: Possible implications for apoptosis resistance in cancer cells Biochim Biophys Acta Gen Subj 2019-12-17 [PMID: 31862471] (WB, Yeast)
• Zhao, SB;Suda, Y;Nakanishi, H;Wang, N;Yoko-O, T;Gao, XD;Fujita, M; Yeast Dop1 is required for glycosyltransferase retrieval from the trans-Golgi network Biochim Biophys Acta Gen Subj 2019-06-01 [PMID: 30981741] (WB, Yeast)
• Audesse, AJ;Dhakal, S;Hassell, LA;Gardell, Z;Nemtsova, Y;Webb, AE; FOXO3 directly regulates an autophagy network to functionally regulate proteostasis in adult neural stem cells PLoS Genet. 2019-04-01 [PMID: 30973875] (WB, Mouse)
• Chung YJ, Luo A, Park KC et al. Iron deficiency anemia reduces cardiac contraction by downregulating RyR2 channels and suppressing SERCA pump activity JCI Insight 2019-02-19 [PMID: 30779710] (WB, Mouse)
• Taylor SM, Giuffre E, Moseley P, Hitchcock PF The MicroRNA, miR-18a, Regulates NeuroD and Photoreceptor Differentiation in the Retina of Zebrafish Dev Neurobiol 2019-02-01 [PMID: 30615274] (WB, Zebrafish)
• Rodger E J, Porteous C M et al. Proteomic Analysis of Liver from Human Lipoprotein(a) Transgenic Mice Shows an Oxidative Stress and Lipid Export Response. Biomed Res Int 2019-01-01 [PMID: 30596094] (WB, Mouse, Human)
• Tsai FJ, Ho MW, Lai CH et al. Evaluation of Oral Antiretroviral Drugs in Mice With Metabolic and Neurologic Complications. Front Pharmacol 2018-09-04 [PMID: 30233379] (WB, Mouse)
• Yang Q, Wang P, Du X et al. Direct repression of IGF2 is implicated in the anti-angiogenic function of microRNA-210 in human retinal endothelial cells Angiogenesis 2018-01-15 [PMID: 29335842] (Human)
• Luevano-Martinez LA, Forni MF, Peloggia J et al. Calorie restriction promotes cardiolipin biosynthesis and distribution between mitochondrial membranes. Mech. Ageing Dev. 2017-02-14 [PMID: 28213011] (WB, Rat)
• Mana G, Clapero F, Panieri E et al. PPFIA1 drives active alpha5beta1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis. Nat Commun. 2016-11-23 [PMID: 27876801] (Human)
• Wang P, Du X, Xiong M et al. Ginsenoside Rd attenuates breast cancer metastasis implicating derepressing microRNA-18a-regulated Smad2 expression Sci Rep 2016-09-19 [PMID: 27641158] (WB, Human)
• Yang Q, Wang P, Cui J et al. Panax notoginseng saponins attenuate lung cancer growth in part through modulating the level of Met/miR-222 axis. J Ethnopharmacol. 2016-08-23 [PMID: 27566197] (WB, Human)
• Yang Q, Cui J, Wang P et al. Changes in interconnected pathways implicating microRNAs are associated with the activity of apocynin in attenuating myocardial fibrogenesis. Eur. J. Pharmacol. 2016-05-09 [PMID: 27174579] (WB, Mouse)
• Belyy A, Tabakova I et al. Roles of Asp179 and Glu270 in ADP-Ribosylation of Actin by Clostridium perfringens Iota Toxin. PLoS One 2015-12-30 [PMID: 26713879] (WB, Yeast)
  
  Details:
  Saccharomyces cerevisiae (Yeast)
• Lin YJ, Ho TJ, Yeh YC et al. Chinese Herbal Medicine Treatment Improves the Overall Survival Rate of Individuals with Hypertension among Type 2 Diabetes Patients and Modulates In Vitro Smooth Muscle Cell Contractility. PLoS One 2015-01-01 [PMID: 26699542] (WB)
• Pei C, Qin S, Wang M, Zhang S. Regulatory mechanism of human vascular smooth muscle cell phenotypic transformation induced by NELIN. Mol Med Rep. 2015-11-01 [PMID: 26458985] (Human)
  
  Details:
  This citation used the FITC version of this antibody.
• Tabolacci C, Cordella M, Turcano L et al. Aloe-emodin exerts a potent anticancer and immunomodulatory activity on BRAF-mutated human melanoma cells Eur. J. Pharmacol. 2015-06-03 [PMID: 26048310] (WB, Human)
• Frey S, Lahmann Y et al. Deletion of Smgpi1 encoding a GPI-anchored protein suppresses sterility of the STRIPAK mutant delta Smmob3 in the filamentous ascomycete Sordaria macrospora. Mol Microbiol 2015-01-08 [PMID: 25989468] (WB, Fungus)
  
  Details:
  Sordaria macrospora
• Beckmann EA, Kohler AM, Meister C et al. Integration of the catalytic subunit activates deneddylase activity in vivo as final step in fungal COP9 signalosome assembly Mol. Microbiol. 2015-04-02 [PMID: 25846252] (WB, Fungi)
• Lehneck R, Elleuche S, Poggeler S. The filamentous ascomycete Sordaria macrospora can survive in ambient air without carbonic anhydrases. Mol. Microbiol. 2014-04-10 [PMID: 24720701] (WB, Simian)
• Cary GA, Yoon SH, Garmendia Torres C et al. Identification and characterization of a drug-sensitive strain enables puromycin-based translational assays in Saccharomyces cerevisiae. Yeast 2014-03-07 [PMID: 24610064] (WB, Yeast)
• Christmann M, Schmaler T, Gordon C et al. Control of Multicellular Development by the Physically Interacting Deneddylases DEN1/DenA and COP9 Signalosome. PLoS Genet 2013-02-01 [PMID: 23408908] (WB, Fungi)
• Pradines E, Hernandez-Rapp J, Villa-Diaz A et al. Pathogenic prions deviate PrP(C) signaling in neuronal cells and impair A-beta clearance Cell Death Dis 2013-01-10 [PMID: 23303130] (WB, Mouse)
• Voigt O, Poggeler S. Autophagy genes Smatg8 and Smatg4 are required for fruiting-body development, vegetative growth and ascospore germination in the filamentous ascomycete Sordaria macrospora. Autophagy. 2013-01-01 [PMID: 23064313] (WB, Yeast)
• Bernard DG, Cheng Y, Zhao Y et al. An allelic mutant series of ATM3 reveals its key role in the biogenesis of cytosolic iron-sulfur proteins in Arabidopsis. Plant Physiol. 2009-10-01 [PMID: 19710232] (WB, Plant)
• Bych K, Netz DJ, Vigani G et al. The essential cytosolic iron-sulfur protein Nbp35 acts without Cfd1 partner in the green lineage. J Biol Chem. 2008-12-01 [PMID: 18957412] (WB, Plant)
• Zheng L, Anderson RE, Agbaga MP et al. Loss of BCL-XL in rod photoreceptors: Increased susceptibility to bright light stress. Invest Ophthalmol Vis Sci. 2006-12-01 [PMID: 17122152] (WB, Mouse)
• Kandasamy MK, McKinney EC, Meagher RB et al. The late pollen-specific actins in angiosperms. Plant J. 1999-06-01 [PMID: 10417720] (WB, Plant)

Reviews for Actin Antibody (NB100-74340) (8)

Average Rating: 4.1

(Based on 8 reviews)

We have 8 reviews tested in 5 species: Human, Mouse, Drosophila, Human and Mouse, Other.

reviewed by: Caitlin Hair Simple Western Mouse 03/27/2018

Summary

• Application: Simple Western
• Sample Tested: brain and spinal cord
• Species: Mouse
• Lot: E

Comments

• Comments: Baseline ran higher on the samples, however the peaks were consistent across samples.

reviewed by: Verified Customer WB Human and Mouse 06/29/2017

Summary

• Application: Western Blot
• Sample Tested: 293ft lysate,NIH 3T3 cell lysate
• Species: Human and Mouse
• Lot: b-2

reviewed by: Alexandra Jette WB Drosophila 03/21/2017

Summary

• Application: Western Blot
• Sample Tested: Whole embryos lysate
• Species: Drosophila
• Lot: B-2

Comments

• Comments: The antibody was diluted 1/10000 and it worked well.

reviewed by: Byron Baron WB Human 12/24/2016

Summary

• Application: Western Blot
• Sample Tested: SW480 (colon)
• Species: Human
• Lot: C1

Comments

• Comments: Primary mouse anti Actin antibody diluted 1:1000 in TBS. Membrane incubation for 2 hours at RT. Secondary IRDye 800CW goat anti mouse antibody diluted 1:10000 in TBS. Membrane incubation for 1 hour at RT. Detection by Li-Cor Odyssey

reviewed by: Bryan Tinsley ICC Other 06/11/2014

Summary

• Application: Immunocytochemistry
• Species: Other

reviewed by: Bryan Tinsley WB Human 06/11/2014

Summary

• Application: Western Blot
• Species: Human

reviewed by: David Sancho ICC Other 10/17/2013

Summary

• Application: Immunocytochemistry
• Sample Tested: Leishmania promastigotes
• Species: Other

reviewed by: Verified Customer WB Other 11/18/2009

Summary

• Application: Western Blot
• Sample Tested: Baking Yeast - Saccharomyces Cerevisiae, Sample Amount: Control Lane - #7 is 1ug
• Species: Other
• Lot: 793-110

FAQs for Actin Antibody (NB100-74340). (Showing 1 - 4 of 4 FAQs).

1. I am interested in buying an Actin antibody to use in Western blot as a loading control. We would like to use it on rat heart samples. Can you make a recommendation?
   • I would recommend the Actin Antibody (mAbGEa) catalog # NB100-74340 . This antibody works great as a loading control in Western blot and has been validated for use with rat samples.
2. One of our customers is interested in one anti-Actin-2, that recognize plant (Arabidopsis and other plants), valid for WB and IP. I have found that Ab at your catalog: Actin Antibody (NB100-74340) - clone: mAbGEa. Please, confirm me if you have available this Ab, and if you have any other that could be valid for this customer.
   • NB100-74340 is an excellent choice for Western blot, but we have not tested it in IP. It is the only antibody that is tested to work in plants, and therefore the only one that I would recommend to your customer.
3. Do you know whether this antibody reacts with Leishmania actin?
   • NB100-74340, was raised against the entire Arabidopsis actin protein and I did an alignment between Arabidopsis Actin and Leishmania Actin, these two sequences are 71.47 percent identical so I can't guarantee this antibody works for Leishmania but since the homology is high, we can predict cross reactivity. I suggest you look into our Innovator's reward program if you are planning to test this antibody in Leishmania. Learn more about our Innovator's Reward program.
4. I would like to know the approximate concentration. 
   • The concentration is 1mg/ml.

Bioinformatics

• Gene Symbol: ACTA1
• Entrez:
  • 533219(Bovine)
  • 58(Human)
  • 11464(Mouse)
  • 29275(Rat)