Actin Gamma 1 Antibody - BSA Free

Western Blot: Actin Gamma 1 Antibody [NB600-533] - Knockdown of DLK in differentiated Neuro-2a cells. Neuro-2a cells were infected with an empty lentiviral vector (pLKO.1) or with lentivirus expressing mouse DLK shRNAs (sh73 and sh69). After infection and selection with puromycin, cells were subjected to differentiation for 24 h before being processed for total RNA extraction and whole-cell extracts. Representative Western blots showing levels of DLK, phospho-JNK (p-JNK), total JNK, phospho-c-Jun (p-c-Jun) and actin in infected Neuro-2a cells. Image collected and cropped by CiteAb from the following publication (//neuraldevelopment.biomedcentral.com/articles/10.1186/s13064-016-0068-8), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Actin Gamma 1 Antibody [NB600-533] - Detection of Actin Gamma 1 (Green) in Hela cells using NB600-533 at a 1:50 dilution. Nuclei (Blue) were counterstained using Hoechst 33258.

Immunohistochemistry: Actin Gamma 1 Antibody [NB600-533] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-Actin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Western Blot: Actin Gamma 1 Antibody [NB600-533] - Whole cell lysate from HeLa (1, 2.5, 5, 15 and 50 ug) and mouse NIH3T3 cells (50 ug), probed with diluted at 0.04 ug/ml.

Western Blot: Actin Gamma 1 Antibody [NB600-533] - Actin Antibody [NB600-533]-Detection of actin in 3T3 (20 ug) lysates. ECL detection 30 seconds. Lane 1 - 1:5,000 dilution Lane 2 - 1:10,000 dilution Lane 3 - 1:15,000 dilution

Western Blot: Actin Gamma 1 Antibody [NB600-533] - Detection of human and mouse Cytoskeletal Actin by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cytoskeletal Actin antibody NB600-533 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Simple Western: Actin Gamma 1 Antibody [NB600-533] - Simple Western lane view shows a specific band for Actin Gamma 1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.

Western Blot: Actin Gamma 1 Antibody [NB600-533] - Validation of RNA-seq data by qRT-PCR & Western blot analyses. a The relative mRNA level of DLK & axon guidance genes in infected cells was analyzed by qRT-PCR, normalized to three housekeeping genes & calculated with the delta delta CT method. The value of mRNA expression for each gene in control cells (pLKO.1) was arbitrarily set to 1. Data are the mean ± SEM (error bars) from three independent experiments carried out in triplicate. *, p 0.05. b Representative Western blots showing levels of DLK, Plxna4, Nrp1 & actin in control & DLK-depleted Neuro-2a cells. c Quantitative densitometric measurements of DLK, Plxna4 & Nrp1 protein levels in infected cells. Results are normalized to actin level in control cells, which were set to 1, & represent mean ± SEM (error bars) from three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27468987), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, Flow, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Actin Gamma 1 Antibody - BSA Free Summary

• Immunogen: The epitope recognized by this antibody maps to the N-terminus of human Actin Gamma 1 [UniProt# P63261]. The N-terminus of Actin Gamma 1 is highly conserved with Beta Actin and preliminary indications are that NB600-533 also recognizes Beta Actin (GeneID 60).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACTG1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunoprecipitation 1:10-1:500
  • Simple Western 1:100
  • Western Blot
• Application Notes: This Actin Gamma 1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western Blot.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 1.0 mg/mL, separated by Size, antibody dilution of 1:100, apparent MW was 49 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.Use in FLOW reported in scientific literature (PMID 28501006).
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Actin Gamma 1 Antibody - BSA Free

• ACT
• ACTB
• ACTG
• actin, cytoplasmic 2
• actin, gamma 1
• cytoskeletal gamma-actin
• deafness, autosomal dominant 20; deafness, autosomal dominant 26
• DFNA20
• DFNA26
• Gamma-actin

Background

Actin, gamma 1 (ACTG1) is a member of the actin protein family, a highly conserved protein group with three main isoforms: alpha, beta and gamma. Alpha actins are mainly expressed in muscle tissues where they are a major constituent of the contractile apparatus, whereas beta and gamma actin proteins are widely expressed in most cell types where they are predominantly involved in cell motility and cytoskeleton maintenance. Actin gamma 1 is a cytoplasmic actin isoform usually found in nonmuscle cells.

Actin Gamma 1 antibodies serve as excellent loading controls, due to the proteins' near ubiquitous expressn and strong conservation across most species. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Actin Gamma 1 antibody serves as an excellent loading control.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Actin Gamma 1 Antibody (NB600-533)(6)

Publications using NB600-533

• Flanagan KC, Alspach E, Pazolli E, Parajuli S. c-Myb and C/EBPb regulate OPN and other senescence-associated secretory phenotype factors. Oncotarget. 2018-01-02 [PMID: 29416593] (WB, Human)
• Luo H, Yao L, Zhang Y, Li R. Liquid chromatography-mass spectrometry-based quantitative proteomics analysis reveals chondroprotective effects of astragaloside IV in interleukin-1b-induced SW1353 chondrocyte-like cells. Biomed. Pharmacother. 2017-05-10 [PMID: 28501006] (FLOW, WB, Human)
• Blondeau A, Lucier JF, Matteau D et al. Dual leucine zipper kinase regulates expression of axon guidance genes in mouse neuronal cells. Neural Dev 2016-07-28 [PMID: 27468987] (WB)
• Duxin JP, Moore HR, Sidorova J et al. Okazaki fragment processing-independent role for human Dna2 enzyme during DNA replication. J Biol Chem 2012-06-01 [PMID: 22570476] (WB, Human)
• Moore HR, Alspach E, Hirsch JL et al. The p38MAPK-MK2-HSP27 Pathway Regulates the mRNA Stability of the Senescence-Associated Secretory Phenotype bioRxiv 2019-06-08 (WB, Human)
• Duxin JP, Dao B, Martinsson P et al. Human Dna2 Is a Nuclear Mitochondrial DNA Maintenance Protein. Mol Cell Biol;29(15):4274-4282. 2009-01-01 [PMID: 19487465]

Bioinformatics

• Gene Symbol: ACTG1
• Entrez:
  • 71(Human)
  • 11465(Mouse)
• Uniprot:
  • P63261(Human)