actin-related protein 2/3 complex subunit 1B Antibody

Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-46219] - Detection of Human and Mouse Arpc1b by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Arpc1b antibody NBP1-46219 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-46219] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-Arpc1b antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. actin-related protein 2/3 complex subunit 1B was also immunoprecipitated by NBP1-46220, which recognizes a downstream epitope.

Immunoprecipitation: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-46219] - Detection of human Arpc1b by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Arpc1b antibody NBP1-46219 (lot NBP1-46219-3) used for IP at 3 ug per reaction. Arpc1b was also immunoprecipitated by a previous lot of this antibody (lot NBP1-46219-2) and rabbit anti-Arpc1b antibody NBP1-46220. For blotting immunoprecipitated Arpc1b, A302-780A was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

actin-related protein 2/3 complex subunit 1B Antibody Summary

• Immunogen: The immunogen for this product maps to a region between residue 272 and 322 of human Actin Related Protein 2/3 complex, Subunit 1B using the numbering given in entry NP_005711.1 (GeneID 10095).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ARPC1B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for actin-related protein 2/3 complex subunit 1B Antibody

• actin related protein 2/3 complex, subunit 1B (41 kD)
• actin related protein 2/3 complex, subunit 1B, 41kDa
• actin-related protein 2/3 complex subunit 1B
• ARC41p41-ARCp40-ARC
• Arp2/3 complex 41 kDa subunit
• ARP2/3 protein complex subunit p41

Background

This gene encodes one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1A. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARPC1B