Activin A Antibody - BSA Free

Western Blot: Activin A Antibody [NBP1-30928] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Inhibin beta A antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Activin A Antibody [NBP1-30928] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: Activin A Antibody [NBP1-30928] - CLEAR CELL OVCA xenograft, using Inhibin beta A antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Western Blot: Activin A Antibody [NBP1-30928] - Western blot analyses of YY1 and inhibin betaA in MDA-231 cells co-transfected with miR-218 mimic and YY1-overexpressing plasmid, or the corresponding controls at 72 h. Image collected and cropped by CiteAb from the following publication (breast-cancer-research.biomedcentral.com/articles/10.1186/s13058-018-1059-y), licensed under a CC-BY license.

Western Blot: Activin A Antibody [NBP1-30928] - miR-218 directly targets INHBB & YY1, & increases INHBA expression. a Relative RNA level of INHBB & INHBA normalized to 18S in MDA-231 transfected with miR-218 or control miRNA mimic at 24 h & 72 h, respectively. ***P < 0.001. b Western blot analyses of inhibin beta B & inhibin beta A in mimic-transfected MDA-231 cells at 48 h. c Relative RNA level of INHBB & INHBA normalized to 18S in MDA-231-bone transfected with anti-miR-218 or control antagomiR at 24 h & 48 h, respectively.. **P < 0.01; ***P < 0.001. d Sequence alignment of miR-218 & its predicted targets INHBB & YY1. e psiCheck2 reporter plasmids & miR-218 mimic or its corresponding control were transfected into indicated cell lines. Renilla & firefly luciferase activity was measured at 48 h. *P < 0.05; n.s. not significant. f Relative RNA level of YY1 normalized to 18S in mimic-transfected MDA-231 cells at 24 h. ***P < 0.001. g Western blot analyses of YY1 & inhibin beta A in MDA-231 cells co-transfected with miR-218 mimic & YY1-overexpressing plasmid, or the corresponding controls at 72 h. h psiCheck2 reporter plasmids & miR-218 mimic or its corresponding control vector were transfected into MDA-231 cells. Renilla & firefly luciferase activity was measured at 48 h. **P < 0.01; n.s. not significant Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30348200), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Activin A Antibody [NBP1-30928] - miR-218-regulated inhibin beta A affects SMAD2/3 signaling in a cell-dependent manner. a & b Western blot analyses of phospho-SMAD2/3 in MDA-231 (a) or MC3T3 (b) that was serum-starved overnight & treated with EV-depleted CM collected from indicated cells for 30 min or 2 h. CM producing cell lines were transfected, PBS washed 48 h after transfection & then incubated with serum-free medium overnight before CM collection & EV depletion by ultracentrifugation. c Western blot analyses of inhibin beta A, inhibin beta B, & inhibin alpha in CM from indicated cell lines that was concentrated with medium concentrator columns. Arrows indicate the position of antigen. Control mimic-transfected MDA-231 whole cell lysate (WCL) was used as positive control for inhibin beta A & inhibin beta B. d Western blot analyses of inhibin beta A monomer or dimer (activin A) in CM from indicated cell lines under non-reducing condition & without boiling the samples. Bottom & top arrows indicate inhibin beta A monomer & dimer, respectively. e Western blot analyses of inhibin alpha in MC3T3 CM concentrated from 20 ml CM. WCL of MCF10A was used as a positive control. f & g Western blot analyses of phospho-SMAD2/3 in MDA-231 (f) or MC3T3 (g) that was serum-starved overnight & treated with CM collected from indicated cells for 30 min or 2 h. For MDA-231 cells (f), recombinant inhibin alpha protein was added to CM as indicated. For MC3T3 cells (g), anti-inhibin alpha antibody was added to CM as indicated Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30348200), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Activin A Antibody [NBP1-30928] - PC-3 conditioned medium (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Inhibin beta A antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ch, Fe, Sh
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Activin A Antibody - BSA Free Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Activin A. The exact sequence is proprietary.
• Localization: Secreted
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Feline (100%), Chicken (100%), Sheep (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: INHBA
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Activin A Antibody - BSA Free

• Activin A
• activin AB alpha polypeptide
• Activin beta-A chain
• erythroid differentiation factor
• Erythroid differentiation protein
• follicle-stimulating hormone-releasing protein
• FSH-releasing protein
• inhibin beta A chain
• inhibin beta A subunit
• Inhibin, beta-1

Background

The inhibin beta A subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumor-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta A subunit forms a homodimer, activin A, and also joins with a beta B subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. Finally, it has been shown that the beta A subunit mRNA is identical to the erythroid differentiation factor subunit mRNA and that only one gene for this mRNA exists in the human genome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Activin A Antibody (NBP1-30928)(6)

Publications using NBP1-30928

• Džepina P, ?ori? M, Kova?i? Perica M, Ani?i? MN et Al. Expression of activin A in liver tissue and the outcome of patients with biliary atresia Front Pediatr 2024-12-02 [PMID: 39618695]
• Tsai MT, Ou SM, Lee KH et al. Circulating Activin A, Kidney Fibrosis, and Adverse Events Clinical journal of the American Society of Nephrology : CJASN 2023-11-20 [PMID: 37983094]
• ElGhazaly M, Collins MO, Ibler AEM, Humphreys D Typhoid toxin hijacks Wnt5a to establish host senescence and Salmonella infection Cell reports 2023-10-31 [PMID: 37792529] (WB, ICC/IF, Human)
• Su X, Ying J, Xiao D et al. Activin A rescues preterm brain injury through a novel Noggin/BMP4/Id2 signaling pathway International Journal of Molecular Medicine 2022-12-14 [PMID: 36524372] (Western Blot, Human)
• Baik SH, Selvaraji S, Fann DY et al. Hippocampal transcriptome profiling reveals common disease pathways in chronic hypoperfusion and aging Aging (Albany NY) 2021-06-15 [PMID: 34074801] (Western Blot, Human)
• Liu X, Cao M, Palomares M et al. Metastatic breast cancer cells overexpress and secrete miR-218 to regulate type I collagen deposition by osteoblasts. Breast Cancer Res. 2018-10-22 [PMID: 30348200] (WB, Human)

Bioinformatics

• Gene Symbol: INHBA