Adropin Antibody - BSA Free

Western Blot: Adropin Antibody [NBP1-26387] - Total protein from Human and Mouse brain was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-Adropin in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Western Blot: Adropin Antibody [NBP1-26387] - Western blot on Adropin overexpression lysate.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.11 mg/ml

Adropin Antibody - BSA Free Summary

• Immunogen: Synthetic peptide made to an internal portion of human Adropin (within residues 10-60). [Swiss-Prot# Q6UWT2]
• Localization: secreted.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ENHO
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/ml
• Application Notes: A band can be seen at 15 kDa in Western Blot. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
  Adropin Overexpression Lysate

• Control Peptide:

  Adropin Peptide (NBP1-26387PEP)

Reactivity Notes

88% sequence identity with bovine protein.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1.11 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Adropin Antibody - BSA Free

• Adropin
• C9orf165
• chromosome 9 open reading frame 165
• energy homeostasis associated
• Energy homeostasis-associated protein
• ENHO
• GAAI470
• UNQ470

Background

Adropin (energy homeostasis-associated protein, ENHO) is a peptide hormone encoded by gene ENHO and its unique ORF generates a 76-residue long secretary peptide which is highly conserved in eutherian (placental) mammals. Adropin is abundantly expressed in liver/brain, and in mouse, hepatic expression as well as circulating adropin concentrations exhibits rapid regulation by fasting (inhibition) and refeeding (stimulation), suggesting regulation through metabolic status signals. Mouse obesity models further confirmed that adropin is involved in metabolic homeostasis and cardiovascular function. Diet-induced as well as genetically induced obesity has been found to be associated with decreased hepatic adropin expression and circulating adropin concentrations. Adropin-knockout mice exhibit increased adiposity and fasting triglycerides (TG), hepatic steatosis, insulin resistance, and increased propensity for impaired glucose tolerance with diet-induced obesity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Adropin Antibody (NBP1-26387)(3)

Publications using NBP1-26387

• Jia L, Liao L, Jiang Y et al. Low-dose adropin stimulates inflammasome activation of macrophage via mitochondrial ROS involved in colorectal cancer progression BMC cancer 2023-10-30 [PMID: 37904094] (WB, Human)
• Lee MJ, Zhu J, An JH et al. A transcriptomic analysis of cerebral microvessels reveals the involvement of Notch1 signaling in endothelial mitochondrial-dysfunction-dependent BBB disruption Fluids and barriers of the CNS 2022-08-26 [PMID: 36028880] (WB, Mouse)
  
  Details:
  Proteins from isolated brain microvessels and brain tissue, dilution used 1:500
• Yang W, Liu L, Wei Y et al. Exercise suppresses NLRP3 inflammasome activation in mice with diet-induced NASH: a plausible role of adropin Laboratory investigation; a journal of technical methods and pathology 2020-12-02 [PMID: 33268842] (WB)

Bioinformatics

• Gene Symbol: ENHO
• Entrez:
  • 375704(Human)
• Uniprot:
  • Q6UWT2()