AgRP/ART Antibody

Immunohistochemistry: AgRP/ART Antibody [NB100-1540] - Staining of PFA-perfused cryosection of Human Hypothalamus. Antibody at 0.05 ug/mL. Antigen retrieval with citrate buffer pH 6 at 80C for 30min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.

• Reactivity: Hu, Po, Ca
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

AgRP/ART Antibody Summary

• Immunogen: Peptide with sequence CRKLGTAMNPCSRT corresponding to C-Terminus according to NP_001129.1, NP_015531.1.
• Epitope: C Terminus
• Specificity: This antibody is expected to recognise both reported isoforms (as represented by NP_001129; NP_015531)
• Predicted Species: Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AGRP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.05 - 0.1 ug/mL
  • Immunohistochemistry-Frozen 0.05 - 0.1 ug/mL
  • Peptide ELISA Detection limit 1:32000
• Application Notes: IHC-Fr: Human hypothalamus shows staining of a group of neuronal cell bodies in the human infundibular (arcuate) nucleus and their varicose axons.
• Control Peptide:

  AgRP/ART Peptide (NB100-1540PEP)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AgRP/ART Antibody

• agouti related protein homolog (mouse)
• agouti-related protein
• AgRP
• Agrt
• AGRTMGC118963
• ART
• ARTagouti (mouse) related protein
• ASIP2

Background

AGRP is the endogenous antagonist of alpha-melanocyte stimulating hormone and has been shown to cause potent stimulation of food intake, and this protein is found in over 90% of Neuropeptide Y containing cells in rats.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AgRP/ART Antibody (NB100-1540)(1)

Publication using NB100-1540

• Breen TL, Conwell IM, Wardlaw SL. Effects of fasting, leptin, and insulin on AGRP and POMC peptide release in the hypothalamus. Brain Res 2005-01-25 [PMID: 15680952]

Bioinformatics

• Gene Symbol: AGRP
• Entrez:
  • 181(Human)
  • 11604(Mouse)
• Uniprot:
  • NP_001129.1(Human)