Aldo-keto Reductase 1C1/AKR1C1 Antibody (859026) [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and human liver tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Aldo-keto Reductase 1C1/AKR1C1 Monoclonal Antibody (Catalog # MAB6529) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Aldo-keto Reductase 1C1/AKR1C1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and human liver tissue, loaded at 0.5 mg/mL. A specific band was detected for Aldo‑keto Reductase 1C1/AKR1C1 at approximately 41 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human Aldo‑keto Reductase 1C1/AKR1C1 Monoclonal Antibody (Catalog # MAB6529) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clone: 859026
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Aldo-keto Reductase 1C1/AKR1C1 Antibody (859026) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Aldo‑keto Reductase 1C1/AKR1C1
  Met1-Tyr323
  Accession # Q04828
• Specificity: Detects human Aldo‑keto Reductase 1C1/AKR1C1 in ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Aldo-keto Reductase 1C3 and 1C4 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: AKR1C1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 1 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Aldo-keto Reductase 1C1/AKR1C1 Antibody (859026) [Unconjugated]

• 20 alpha-hydroxysteroid dehydrogenase
• 20-ALPHA-HSD
• 20-alpha-hydroxysteroid dehydrogenase
• 2-ALPHA-HSD
• AKR1C1
• Aldo-keto Reductase 1C1
• aldo-keto reductase family 1 member C1
• aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1; 20-alpha(3-alpha)-hydroxysteroid dehydrogenase)
• AldoketoReductase 1C1
• C9
• Chlordecone reductase homolog HAKRC
• DD1/DD2
• DD1MGC8954
• DDH1
• DDHH-37
• dihydrodiol dehydrogenase 1
• Dihydrodiol dehydrogenase 1/2
• dihydrodiol dehydrogenase isoform DD1
• EC 1.1.1
• EC 1.1.1.-
• EC 1.1.1.112
• EC 1.1.1.149,2-ALPHA-HSD
• EC 1.3.1.20
• HAKRC
• HAKRCDDH1aldo-keto reductase C
• HBAB
• hepatic dihydrodiol dehydrogenase
• High-affinity hepatic bile acid-binding protein
• Indanol dehydrogenase
• MBAB
• Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase
• type II 3-alpha-hydroxysteroid dehydrogenase

Background

AKR1C1 (20-alpha -hydroxysteroid dehydrogenase, 20‑ alpha ‑HSD) is a member of aldo-keto reductase (AKR) superfamily. AKRs perform the NAD(P)H-dependent reduction of carbonyl groups (1). Four AKR1C isoforms (AKR1C1-C4) are known to exist in humans. They are all highly expressed in the liver. Three isoforms, excluding AKR1C4, have a wider expression pattern including prostate, testes, uterus, mammary gland, and haemopoietic progenitors (2). These enzymes are able to accept various natural steroids as substrates, including 3-, 7-, and 20‑ketosteroids (3). They can also activate prodrugs such as synthetic steroid hormone tibolone by converting it into active 3 alpha / beta -hydroxy form (4). They are recognized as phase I drug-metabolizing enzymes involved in the maintenance of steroid homeostasis, prostaglandin metabolism, and metabolic activation of polycyclic aromatic hydrocarbons (5). Their reactions introduce a hydroxyl group into the product making it available for sulfonation and glucuronidation by phase II enzyme. Elevated expression of these enzymes is related to cancer with hormone-dependent malignancies (6, 7). Increased levels of expression of AKR1C1 parallels increased cell proliferation activity in human colon cancer cells. It has been shown to be associated with oncogenic potential and proproliferative effects. It is also involved in cancer cell chemoresistance.

1. Jez, J.M. et al. (1997) Biochem J. 326:499.
2. Penning, T.M. et al. (2000) Biochem. J. 351:67.
3. Rizner, T.L. et al. (2003) Endocrinology. 144:2922.
4. Steckelbroeck, S. et al. (2006) J. Pharmacol. Exp. Ther. 316:1300.
5. Penning, T.M. et al. (2004) Mol. Cell. Endocrinol. 1784:1342.
6. Penning, T.M. and M.C. Byrns (2009) Ann. N. Y. Acad. Sci. 1155:33.
7. Baumann, D.R. et al. (2004) Drug News Perspect. 17:563.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aldo-keto Reductase 1C1/AKR1C1 Antibody (MAB6529)(2)

Publications using MAB6529

• Wensen Jiang, Juliane D. Glaeser, Khosrowdad Salehi, Giselle Kaneda, Pranav Mathkar, Anton Wagner et al. Single-cell atlas unveils cellular heterogeneity and novel markers in human neonatal and adult intervertebral discs iScience 2022-07-15 [PMID: 35754733]
• Wensen Jiang, Juliane D. Glaeser, Khosrowdad Salehi, Giselle Kaneda, Pranav Mathkar, Anton Wagner et al. Single-cell atlas unveils cellular heterogeneity and novel markers in human neonatal and adult intervertebral discs iScience 2022-07-15 [PMID: 35754733] (Immunohistochemistry, Human)

Bioinformatics

• Gene Symbol: AKR1C1
• Uniprot:
  • Q04828()