alpha-Actinin 1 Antibody

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat a-Actinin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8279) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for a-Actinin 1 at approximately 100-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

a-Actinin 1 was detected in immersion fixed A172 human glioblastoma cell line using Goat Anti-Human/Mouse/Rat a-Actinin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8279) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

alpha-Actinin 1 Antibody Summary

• Immunogen: E. coli-derived recombinant human alpha -Actinin 1
  Asp272-Thr500
  Accession # P12814
• Specificity: Detects human, mouse and rat alpha -Actinin 1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ACTN1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha-Actinin 1 Antibody

• actinin 1 smooth muscle
• actinin, alpha 1
• ACTN1
• alpha Actinin 1
• alphaActinin 1
• alpha-Actinin 1
• Alpha-actinin cytoskeletal isoform
• alpha-actinin-1
• BDPLT15
• F-actin cross-linking protein
• FLJ40884
• FLJ54432
• Non-muscle alpha-actinin-1

Background

Alpha-Actinin 1 (ACTN1) is a member of the spectrin superfamily with a predicted molecular weight of approximately 100 kDa.  It is 892 amino acids in length and shares 99% aa identity with mouse and rat ACTN1.  Multiple splice forms exist, resulting in 4 distinct isoforms with different tissue expression patterns.  ACTN1 binds to F-actin as well as other cytoskeletal and signaling proteins and plays an important role in both cell motility and muscle contraction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha-Actinin 1 Antibody (AF8279)(6)

Publications using AF8279

• Haibi Su, Jie Xu, Zhenghua Su, Chenxi Xiao, Jinghuan Wang, Wen Zhong et al. Poly (ADP-ribose) polymerases 16 triggers pathological cardiac hypertrophy via activating IRE1 alpha –sXBP1–GATA4 pathway Cellular and Molecular Life Sciences 2023-06-01 [PMID: 37219631] (Immunocytochemistry, Rat)
• Pinja Kettunen, Angelina Lesnikova, Noora Räsänen, Ravi Ojha, Leena Palmunen, Markku Laakso et al. SARS-CoV-2 Infection of Human Neurons Is TMPRSS2 Independent, Requires Endosomal Cell Entry, and Can Be Blocked by Inhibitors of Host Phosphoinositol-5 Kinase Journal of Virology 2023-04-27 [PMID: 37039676]
• Shuzhen Liu, Guoyan An, Qing Cao, Tong Li, Xinyu Jia, Lei Lei The miR-106b/NR2F2-AS1/PLEKHO2 Axis Regulates Migration and Invasion of Colorectal Cancer through the MAPK Pathway International Journal of Molecular Sciences 2021-05-30 [PMID: 34070923]
• Colpan M, Iwanski J, Gregorio CC CAP2 is a regulator of actin pointed end dynamics and myofibrillogenesis in cardiac muscle Communications biology 3/19/2021 [PMID: 33742108]
• Haseli S, Deubel S et al. Cardiomyocyte Contractility and Autophagy in a Premature Senescence Model of Cardiac Aging. Oxid Med Cell Longev 8/5/2020 [PMID: 32377307]
• RC Ahrens-Nic, CT Pappas, GP Farman, RM Mayfield, TM Larrinaga, L Medne, A Ritter, ID Krantz, C Murali, KY Lin, JH Berger, SW Yum, CK Carreon, CC Gregorio Disruption of cardiac thin filament assembly arising from a mutation in LMOD2: A novel mechanism of neonatal dilated cardiomyopathy Sci Adv, 2019-09-04;5(9):eaax2066. 2019-09-04 [PMID: 31517052] (Western Blot, Human)

Bioinformatics

• Gene Symbol: ACTN1
• Uniprot:
  • P12814()