Src [p Tyr419] Antibody [Unconjugated]

Western blot shows lysates of MDA-MB-468 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 50 µM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Src (Y419) at approximately 56 - 61 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Src phosphorylated at Y419 was detected in immersion fixed paraffin-embedded sections of human colon using Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Src phosphorylated at Y419 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Src phosphorylated at Y419 was detected in immersion fixed paraffin-embedded sections of human liver array using Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of MBA‑MB‑468 human breast cancer cell line untreated (-) or treated (+) with 50 µM Pervanadate (PV) for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Src (Y419) at approximately 64 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Yap heterogeneity is unrelated to markers examinedAll panels show examples of confocal stains of lung slices from KP mice, 14 weeks after infection. (A, B) Adjacent sections stained for E-cad, ZO-1, and either Yap (A), or phospho-Yap (B). (C, D) Adjacent sections stained for E-cad, SpC, and either Yap (C), or phospho-Acc (pAcc) (D). (E, F) Adjacent sections stained for E-cad, SpC, and either Yap (E), or phospho-Src (pSrc) (F). (G, H) Adjacent sections stained for E-cad, SpC, and either Yap (E), or diphospho-Erk (F). (I) Section stained for YAP (red), Vimentin (green), and DNA (blue). (J) Section stained for YAP (red), ZO-1 (green), and E-Cad (blue). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29340023), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

Src [p Tyr419] Antibody [Unconjugated] Summary

• Immunogen: Phosphopeptide containing human Src Y419 site
• Modification: p Tyr419
• Specificity: Detects human Src family members when phosphorylated at sites corresponding to Y419 of human Src in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SRC
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Src [p Tyr419] Antibody [Unconjugated]

• ASV
• c-Src
• EC 2.7.10
• EC 2.7.10.2
• pp60c-src
• Rous sarcoma
• RSVgp4
• Src
• tyrosine kinase pp60c-src
• tyrosine-protein kinase SRC-1
• v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
• v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)

Background

Src and other Src family protein tyrosine kinases are proto-oncogenes that play key roles in regulating cell growth and differentiation. Src family activity is regulated by tyrosine phosphorylation at two sites with opposing effects. Phosphorylation at Y419 of human Src up-regulates kinase activity, while phosphorylation at Y530 inactivates Src.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Src Antibody (AF2685)(10)

Publications using AF2685

• Ana Ruiz-Saenz, Chloe E. Atreya, Changjun Wang, Bo Pan, Courtney A. Dreyer, Diede Brunen et al. A reversible SRC-relayed COX2 inflammatory program drives resistance to BRAF and EGFR inhibition in BRAFV600E colorectal tumors Nature Cancer 2/9/2023 [PMID: 36759733]
• Lisa Rösch, Sonja Herter, Sara Najafi, Johannes Ridinger, Heike Peterziel, Jindrich Cinatl et al. ERBB and P‐glycoprotein inhibitors break resistance in relapsed neuroblastoma models through P‐glycoprotein Molecular Oncology 1/1/2023 [PMID: 36181342]
• Yen-Lin Huang, Ching-Yeu Liang, Danilo Ritz, Ricardo Coelho, Dedy Septiadi, Manuela Estermann et al. Collagen-rich omentum is a premetastatic niche for integrin alpha 2-mediated peritoneal metastasis eLife 10/7/2020 [PMID: 33026975]
• J Ramírez-Ri, E Leal-Orta, E Martínez-B, C Ortiz-Mend, F Breton-Mor, A Herrera-To, I Elizalde-A, P Cortes-Rey, R Thompson-B, E Perez Sala Circulating extracellular vesicles from patients with breast cancer enhance migration and invasion via a Src?dependent pathway in MDA?MB?231 breast cancer cells Mol Med Rep, 2020-06-18;0(0):. 2020-06-18 [PMID: 32582965] (Western Blot, Human)
• Y Mao, S Sun, KD Irvine Role and regulation of Yap in KrasG12D-induced lung cancer Oncotarget, 2017-12-02;8(67):110877-110889. 2017-12-02 [PMID: 29340023] (IHC-Fr, Mouse)
• Eri Takiguchi, Masato Nishimura, Ayuka Mineda, Takako Kawakita, Akiko Abe, Minoru Irahara Growth inhibitory effect of the Src inhibitor dasatinib in combination with anticancer agents on uterine cervical adenocarcinoma cells Experimental and Therapeutic Medicine 8/28/2017 [PMID: 29067110]
• JH Pak, Q Bashir, IK Kim, SJ Hong, S Maeng, YY Bahk, TS Kim Clonorchis sinensis excretory-secretory products promote the migration and invasion of cholangiocarcinoma cells by activating the integrin ?4-FAK/Src signaling pathway Mol. Biochem. Parasitol, 2017-03-08;0(0):. 2017-03-08 [PMID: 28286026] (Western Blot, Human)
• Cahoon J, Rai R, Carroll L, Uehara H, Zhang X, O'Neil C, Medina R, Das S, Muddana S, Olson P, Nielson S, Walker K, Flood M, Messenger W, Archer B, Barabas P, Krizaj D, Gibson C, Li D, Koh G, Gao G, Stitt A, Ambati B Intravitreal AAV2.COMP-Ang1 Prevents Neurovascular Degeneration in a Murine Model of Diabetic Retinopathy. Diabetes, 2015-09-04;64(12):4247-59. 2015-09-04 [PMID: 26340930] (Western Blot, Human)
• Ye F, Zhou F, Nithianantham S, Chandran B, Yu X, Weinberg A, Gao S Kaposi's sarcoma-associated herpesvirus induces rapid release of angiopoietin-2 from endothelial cells. J Virol, 2013-03-27;87(11):6326-35. 2013-03-27 [PMID: 23536671] (Western Blot, Human)
• Matsuo K, Nishimura M, Bottsford-Miller JN, Huang J, Komurov K, Armaiz-Pena GN, Shahzad MM, Stone RL, Roh JW, Sanguino AM, Lu C, Im DD, Rosenshien NB, Sakakibara A, Nagano T, Yamasaki M, Enomoto T, Kimura T, Ram PT, Schmeler KM, Gallick GE, Wong KK, Frumovitz M, Sood AK Targeting Src in Mucinous Ovarian Carcinoma. Clin. Cancer Res., 2011-07-07;17(16):5367-5378. 2011-07-07 [PMID: 21737505] (IHC-P, Human, Mouse)

Review for Src Antibody (AF2685) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 10/11/2018

Summary

• Application: Western Blot
• Sample Tested: Breast cancer cells
• Species: Human
• Lot: VEF0615091

Bioinformatics

• Gene Symbol: SRC