alpha-Actinin 1 Antibody

Western Blot: alpha-Actinin 1 Antibody [NBP2-89158] - Analysis of alpha Actinin on different lysates using anti-alpha Actinin antibody at 1/500 dilution. Positive control: Lane 1: A431 Lane 2: Rat colon tissue Lane 3: Mouse colon tissue

Immunocytochemistry/Immunofluorescence: alpha-Actinin 1 Antibody [NBP2-89158] - Staining alpha Actinin in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody [NBP2-89158] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-alpha-Actinin 1 antibody. Counter stained with hematoxylin.

Flow Cytometry: alpha-Actinin 1 Antibody [NBP2-89158] - Analysis of MG-63 cells with alpha Actinin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: alpha-Actinin 1 Antibody [NBP2-89158] - Staining alpha Actinin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: alpha-Actinin 1 Antibody [NBP2-89158] - Staining alpha Actinin in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody [NBP2-89158] - Analysis of paraffin-embedded human colon cancer tissue using anti-alpha Actinin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody [NBP2-89158] - Analysis of paraffin-embedded mouse colon tissue using anti-alpha Actinin antibody. Counter stained with hematoxylin.

Product Discontinued

alpha-Actinin 1 Antibody Summary

• Immunogen: Recombinant protein within Human alpha-Actinin 1 aa 388-619 / 892. (SwissProt: P12814 Human; SwissProt: Q7TPR4 Mouse; SwissProt: Q9Z1P2 Rat)
• Localization: Plasma membrane. Cytoskeleton.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACTN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50 -1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 103 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for alpha-Actinin 1 Antibody

• actinin 1 smooth muscle
• actinin, alpha 1
• ACTN1
• alpha Actinin 1
• alphaActinin 1
• alpha-Actinin 1
• Alpha-actinin cytoskeletal isoform
• alpha-actinin-1
• BDPLT15
• F-actin cross-linking protein
• FLJ40884
• FLJ54432
• Non-muscle alpha-actinin-1

Background

Alpha Actinin is an actin binding protein present in both muscle and non-muscle cells. It has a molecular weight of 100,000 daltons and forms dimers in solution. In normal skeletal muscle alpha actinin is associated with the z-discs that define muscle sarcomeres. In smooth muscle alpha actinin has been detected in dense bodies and plaques characteristic of the tissue. Immunofluorescent labeling of a large variety of cells using antibody to alpha actinin reveals extensive association of the proteins with actin containing stress fibers and in particular with their membrane bound termini.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACTN1