alpha-Methylacyl-CoA Racemase/AMACR Antibody [Unconjugated]

Western blot shows lysates of human kidney tissue, human liver tissue, and human prostate tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human a-Methylacyl-CoA Racemase/AMACR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7508) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for a-Methylacyl-CoA Racemase/AMACR at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of human kidney tissue, human liver tissue, and human prostate tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human a-Methylacyl-CoA Racemase/AMACR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7508) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for a-Methylacyl-CoA Racemase/AMACR at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

a-Methylacyl-CoA Racemase/AMACR was detected in immersion fixed paraffin-embedded sections of human prostate gland using Sheep Anti-Human a-Methylacyl-CoA Racemase/AMACR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7508) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

alpha-Methylacyl-CoA Racemase/AMACR Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human alpha ‑Methylacyl‑CoA Racemase/AMACR
  Met1-Leu382
  Accession # Q9UHK6
• Specificity: Detects human alpha ‑Methylacyl‑CoA Racemase/AMACR in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: AMACR
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha-Methylacyl-CoA Racemase/AMACR Antibody [Unconjugated]

• 2-methylacyl-CoA racemase
• alpha-Methylacyl-CoA Racemase
• alphaMethylacylCoARacemase
• AMACR
• AMACRD
• CBAS4
• EC 5.1.99.4
• RACE
• RM

Background

AMACR (Alpha-MethylAcyl-CoA Racemase; also 2-methylacyl racemase) is a 43-46 kDa member of the CaiB/BaiF CoA-transferase family of enzymes. It is widely expressed, being found in fibroblasts, hepatocytes, plus tumorigenic prostatic and colonic epithelium. Within these cells, it is localized to peroxisomes (organelles that participate in the breakdown fatty acids into 2-carbon blocks) and occasionally mitochondria, and appears to racemize 2-methyl-branched fatty acids. This ability is necessary for the degradation of branched fatty acids such as C19 dietary pristanic acid. Pristanic acid occurs in both an S- and R-methylated stereoisomer, but can only be initially degraded in the S- isomeric form. AMACR converts the R- to the S-isoform, initiating fatty acid processing. Human AMACR(-IA) is 382 amino acids (aa) in length. It contains an N-terminal mitochondrial targeting sequence (aa 22-85) that overlaps the enzymatic region (aa 53-231), and a C-terminal peroxisomal targeting motif (aa 379-382). There are multiple potential splice variants. Over aa 132-382, there are three aa substitutions, one that is 66 aa in length (AMACR‑IB), a second that is 147 aa in length (AMACR-IIB), and a third that is 98 aa in length. Over aa 249-382, there are two aa substitutions, one that is 13 aa in length (AMACR‑IIAs), and another that is 41 aa in length (AMACR-IIA). There is also a sixth potential splice variant that shows a 16 aa substitution for aa 378-382. Full-length human AMACR(-IA) shares 77% aa sequence identity with mouse AMACR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha-Methylacyl-CoA Racemase/AMACR Antibody (AF7508)(1)

Publication using AF7508

• J Wang, G He, Q Yang, L Bai, B Jian, Q Li, Z Li B-cell lymphoma 2 is associated with advanced tumor grade and clinical stage, and reduced overall survival in young Chinese patients with colorectal carcinoma Oncol Lett, 2018-04-13;15(6):9009-9016. 2018-04-13 [PMID: 29844818] (IHC-P, Western Blot, Human)

Bioinformatics

• Gene Symbol: AMACR
• Uniprot:
  • Q9UHK6(Human)