p63/TP73L Antibody [Unconjugated]

p63/TP73L was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

p63/TP73L was detected in immersion fixed SCC-25 human tongue carcinoma cell line using Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Analysis of p73 and p63 co-expression in human and murine skin.(A) Scatter plot of TP63 versus TP73 RNA-seq expression [units = transcripts per million (TPM)] by human tissue type (n = 37) from the Human Protein Atlas (172 total samples) [30]. Mean expression (TPM + 0.1) for each tissue is plotted on a log2 scale with a LOESS smooth local regression line (gray). Correlation between TP63 and TP73 was quantified using Spearman’s rank correlation coefficient (rs). (B) Representative micrographs of H&E and immunofluorescence (IF) staining on serial human (top) and mouse (bottom) skin sections; DAPI (blue), p63 alpha (green), and p73 (red). Regions of the skin in micrographs are labeled as: interfollicular epidermis (IFE), hair follicle (HF), outer root sheath (ORS), HF bulge (Bu), hair bulb (HB), sebaceous gland (SG), hair shaft (HS), and arrector pili muscle (APM). Scale bars represent 200 μm for human and 50 μm for murine tissue. See also S1 and S2 Figs. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31216312), licensed under a CC-BY license. Not internally tested by R&D Systems.

Analysis of delta Np73 in epidermal programming using an induced basal keratinocyte (iKC) model system.(A) Immunoblot of KLF4, p63 alpha , and p73 protein expression in neonatal human dermal fibroblast (HDFn) cells infected with lentivirus encoding delta Np73 isoforms ( delta Np73 alpha and delta Np73 beta ) or empty vector control in combination with KLF4 and delta Np63 alpha . Cells were grown for 3 days and protein was harvested for immunoblot analysis. (B) Bar graphs of RNA expression for the indicated keratinocyte genes in HDFn cells infected in (A). Cells were grown for 3 days and RNA was harvested for qRT-PCR analysis. Expression data are represented as the fold increase relative to control. The mean of three replicates is shown with error bars representing SEM. *p-value < 0.05, **p-value < 0.01, ***p-value < 0.001. (C) Principal component analysis (PCA) plot of RNA-seq from HDFn cells infected with lentivirus encoding delta Np73 beta or empty vector control in combination with KLF4 and delta Np63 alpha . Cells were grown for 6 days and RNA was harvested for RNA-seq analysis. The percentage of variance contributed by each PC is listed in parentheses. (D and E) Tables listing the enriched Genome Ontology (GO) categories and pathways among the top 250 genes contributing to PC1 from (C). (F) Heatmap with the expression of a set of 44 genes that underlie the enrichment of GO categories from (D). Genes are annotated based on known roles in iKC-related processes (gray box) and the presence of a p63/p73 ChIP-seq peak within 50 kb of its TSS in multiple basal cell types (brown box). See also S5 and S6 Figs and S2–S9 Tables. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31216312), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated

p63/TP73L Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human p63/TP73L
  Met40-Cys339
  Accession # Q9H3D4
• Specificity: Detects human p63/TP73L in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TP63
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for p63/TP73L Antibody [Unconjugated]

• amplified in squamous cell carcinoma
• Chronic ulcerative stomatitis protein
• CUSP
• EEC3
• EEC3B(p51B)
• Keratinocyte transcription factor KET
• KET
• KETRHS
• LMS
• OFC8
• OFC8SHFM4TP73LNBP
• p40
• p51
• p51tumor protein p53-like
• p53CP
• p63
• p63AIS
• p73H
• p73L
• p73LB(p51A)
• SHFM4
• TP53CP
• TP53L
• TP63
• TP73L
• Transformation-related protein 63
• tumor protein 63
• tumor protein p53-competing protein
• tumor protein p63 deltaN isoform delta
• tumor protein p63
• Tumor protein p73-like

Background

Tumor Protein 63 (p63), also named TP73L, TP63, p51, p40 or KET, is a p53 homolog. It is one of several proteins that are produced from a single gene using two promoters and alternative splicing of the primary RNA transcript. p63 is highly expressed in embryonic ectoderm and in the nuclei of basal regenerative cells of many epithelial tissues in the adult. p63 is suggested to play a role in development, epithelial cell maintenance and tumorigenesis (1‑3).

1. Harms, K. et al. (2004), Cell Mol. Life Sci. 61(7):822.
2. Koster, M.I. and D.R. Roop, J. Dermatol. Sci. 34(1):3.
3. Benard, J. et al. (2003) Hum. Mutat. 21(3):182.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p63/TP73L Antibody (AF1916)(24)

Publications using AF1916

• Plumber, SA;Tate, T;Al-Ahmadie, H;Chen, X;Choi, W;Basar, M;Lu, C;Viny, A;Batourina, E;Li, J;Gretarsson, K;Alija, B;Molotkov, A;Wiessner, G;Lee, BHL;McKiernan, J;McConkey, DJ;Dinney, C;Czerniak, B;Mendelsohn, CL; Rosiglitazone and trametinib exhibit potent anti-tumor activity in a mouse model of muscle invasive bladder cancer Nature communications 2024-08-02 [PMID: 39095358] (Immunohistochemistry, Mouse)
• Tietze, E;Barbosa, AR;Araujo, B;Euclydes, V;Spiegelberg, B;Cho, HJ;Lee, YK;Wang, Y;McCord, A;Lorenzetti, A;Feltrin, A;van de Leemput, J;Di Carlo, P;Ursini, G;Benjamin, KJ;Brentani, H;Kleinman, JE;Hyde, TM;Weinberger, DR;McKay, R;Shin, JH;Sawada, T;Paquola, ACM;Erwin, JA; Human archetypal pluripotent stem cells differentiate into trophoblast stem cells via endogenous BMP5/7 induction without transitioning through naive state Scientific reports 2024-02-08 [PMID: 38332235] (ICC, Human)
• Tate, T;Plumber, SA;Al-Ahmadie, H;Chen, X;Choi, W;Lu, C;Viny, A;Batourina, E;Gartensson, K;Alija, B;Molotkov, A;Wiessner, G;McKiernan, J;McConkey, D;Dinney, C;Czerniak, B;Mendelsohn, CL; Combined Mek inhibition and Pparg activation Eradicates Muscle Invasive Bladder cancer in a Mouse Model of BBN-induced Carcinogenesis bioRxiv : the preprint server for biology 2023-08-21 [PMID: 37662238] (IHC, Mouse)
• Assou, S;Ahmed, E;Morichon, L;Nasri, A;Foisset, F;Bourdais, C;Gros, N;Tieo, S;Petit, A;Vachier, I;Muriaux, D;Bourdin, A;De Vos, J; The Transcriptome Landscape of the In Vitro Human Airway Epithelium Response to SARS-CoV-2 International journal of molecular sciences 2023-07-27 [PMID: 37569398] (ICC, Human)
• Ruan D, Ye Z, Yuan S et al. Human Early Syncytiotrophoblasts Are Highly Susceptible to SARS-CoV-2 Infection Cell Reports Medicine 11/1/2022 [PMID: 36495872]
• Adithya Sridhar, Josse A. Depla, Lance A. Mulder, Eveliina Karelehto, Lieke Brouwer, Leonie Kruiswijk et al. Enterovirus D68 Infection in Human Primary Airway and Brain Organoids: No Additional Role for Heparan Sulfate Binding for Neurotropism Microbiology Spectrum 2022-10-26 [PMID: 36154279]
• Yoshiki Nakashima, Chika Miyagi-Shiohira, Issei Saitoh, Masami Watanabe, Masayuki Matsushita, Masayoshi Tsukahara et al. Induced hepatic stem cells are suitable for human hepatocyte production iScience 10/21/2022 [PMID: 36147945]
• Z Zhang, H Peng, J Lai, L Jiang, L Wang, S Jin, K Fan, Z Zhang, C Zhao, D Deng, P Zhao, Z Gao, S Yu Differential susceptibility to SARS-CoV-2 in the normal nasal mucosa and in chronic sinusitis European Journal of Immunology, 2022-05-14;0(0):. 2022-05-14 [PMID: 35524548] (IHC, Human)
• MG Dalghi, WG Ruiz, DR Clayton, N Montalbett, SL Daugherty, JM Beckel, MD Carattino, G Apodaca Functional roles for Piezo1 and Piezo2 in urothelial mechanotransduction and lower urinary tract interoception JCI Insight, 2021-10-08;0(0):. 2021-10-08 [PMID: 34464353] (IHC, Mouse)
• LY Li, Q Yang, YY Jiang, W Yang, Y Jiang, X Li, M Hazawa, B Zhou, GW Huang, XE Xu, S Gery, Y Zhang, LW Ding, AS Ho, ZS Zumsteg, MR Wang, MJ Fullwood, SJ Freedland, SJ Meltzer, LY Xu, EM Li, HP Koeffler, DC Lin Interplay and cooperation between SREBF1 and master transcription factors regulate lipid metabolism and tumor-promoting pathways in squamous cancer Nature Communications, 2021-07-16;12(1):4362. 7/16/2021 [PMID: 34272396]
• Zania Diabasana, Jeanne-Marie Perotin, Randa Belgacemi, Julien Ancel, Pauline Mulette, Claire Launois et al. Chr15q25 Genetic Variant rs16969968 Alters Cell Differentiation in Respiratory Epithelia International Journal of Molecular Sciences 2021-06-22 [PMID: 34206324]
• Z Diabasana, JM Perotin, R Belgacemi, J Ancel, P Mulette, G Delepine, P Gosset, U Maskos, M Polette, G Deslée, V Dormoy Nicotinic Receptor Subunits Atlas in the Adult Human Lung Int J Mol Sci, 2020-10-09;21(20):. 2020-10-09 [PMID: 33050277] (IHC, Human)
• J Ancel, R Belgacemi, JM Perotin, Z Diabasana, S Dury, M Dewolf, A Bonnomet, N Lalun, P Birembaut, M Polette, G Deslée, V Dormoy Sonic hedgehog signalling as a potential endobronchial biomarker in COPD Respir. Res., 2020-08-07;21(1):207. 2020-08-07 [PMID: 32767976] (IHC, Human)
• Chen Dong, Mariana Beltcheva, Paul Gontarz, Bo Zhang, Pooja Popli, Laura A Fischer et al. Derivation of trophoblast stem cells from naïve human pluripotent stem cells eLife 2020-02-12 [PMID: 32048992]
• Y Liu, N Yin, X Wang, A Khoor, V Sambandam, AB Ghosh, ZA Fields, NR Murray, V Justilien, AP Fields Chromosome 3q26 Gain Is an Early Event Driving Coordinated Overexpression of the PRKCI, SOX2, and ECT2 Oncogenes in Lung Squamous Cell Carcinoma Cell Rep, 2020-01-21;30(3):771-782.e6. 2020-01-21 [PMID: 31968252] (IHC, Mouse)
• B You, F Mercier, E Assenat, C Langlois-J, O Glehen, J Soulé, L Payen, V Kepenekian, M Dupuy, F Belouin, E Morency, V Saywell, M Flacelière, P Elies, P Liaud, T Mazard, D Maucort-Bo, W Tan, B Vire, L Villeneuve, M Ychou, M Kohli, D Joubert, A Prieur The oncogenic and druggable hPG80 (Progastrin) is overexpressed in multiple cancers and detected in the blood of patients EBioMedicine, 2019-12-24;51(0):102574. 2019-12-24 [PMID: 31877416] (IHC, Human)
• R Belgacemi, E Luczka, J Ancel, Z Diabasana, JM Perotin, A Germain, N Lalun, P Birembaut, X Dubernard, JC Mérol, G Delepine, M Polette, G Deslée, V Dormoy Airway epithelial cell differentiation relies on deficient Hedgehog signalling in COPD EBioMedicine, 2019-12-23;51(0):102572. 2019-12-23 [PMID: 31877414] (IHC, Human)
• Parikh AS, Puram SV, Faquin WC et al. Immunohistochemical quantification of partial-EMT in oral cavity squamous cell carcinoma primary tumors is associated with nodal metastasis Oral Oncol. 11/5/2019 [PMID: 31704557]
• Meng-Hsiung Hsieh, Joshua H. Choe, Jashkaran Gadhvi, Yoon Jung Kim, Marcus A. Arguez, Madison Palmer et al. p63 and SOX2 Dictate Glucose Reliance and Metabolic Vulnerabilities in Squamous Cell Carcinomas Cell Reports 2019-08-13 [PMID: 31412252]
• Preston D. Crowell, Jonathan J. Fox, Takao Hashimoto, Johnny A. Diaz, Héctor I. Navarro, Gervaise H. Henry et al. Expansion of Luminal Progenitor Cells in the Aging Mouse and Human Prostate Cell Reports 8/6/2019 [PMID: 31390564]
• JS Beeler, CB Marshall, PI Gonzalez-E, TM Shaver, GL Santos Gua, ST Lea, KN Johnson, H Jin, BJ Venters, ME Sanders, JA Pietenpol p73 regulates epidermal wound healing and induced keratinocyte programming PLoS ONE, 2019-06-19;14(6):e0218458. 2019-06-19 [PMID: 31216312] (ChIP, Human)
• Eveliina Karelehto, Cosimo Cristella, Xiao Yu, Adithya Sridhar, Rens Hulsdouw, Karen de Haan et al. Polarized Entry of Human Parechoviruses in the Airway Epithelium Frontiers in Cellular and Infection Microbiology 2018-08-22 [PMID: 30211126]
• Puram Sidharth V, Tirosh Itay, Parikh Anuraag S et al. Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Head and Neck Cancer. Cell 12/14/2018 [PMID: 29198524]
• F. Aura Kullmann, Dennis R. Clayton, Wily G. Ruiz, Amanda Wolf-Johnston, Christian Gauthier, Anthony Kanai et al. Urothelial proliferation and regeneration after spinal cord injury American Journal of Physiology-Renal Physiology 2017-07-01 [PMID: 28331065]

Review for p63/TP73L Antibody (AF1916) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Tony Reyes IHC-P Mouse 09/23/2020

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse lung paraffin embedded sections,mouse lung tissue
• Species: Mouse
• Lot: KFX0120011

Comments

• Comments: p63/TP73L was detected in paraffin-embedded mouse tissue from Lkb1fl/fl; Ptenfl/fl and Sox2LSL/LSL ;Nkx2-1fl/fl; Lkb1fl/fl (SNL) mice, which present with lung squamous cell carcinoma (LSCC) tumors, using the Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) overnight at 4℃ in blocking buffer at a dilution of 1/400. Citrate buffer pH 6.0 was used for high temperature antigen retrieval for 15 minutes in a pressure cooker. Tissue was stained using a HRP-conjugated secondary antibody and DAB staining and counterstained with hematoxylin (blue). P63 positivity was seen in the basal cells found in the trachea of mice and in LSCC tumors but not in small cell lung cancer (SCLC) tumors from Rb1/p53/Myc and Rb1/p53/Rbl2 mice or in mucinous adenocarcinoma tumors found in SNL mice.

Bioinformatics

• Gene Symbol: TP63
• Uniprot:
  • Q9H3D4()