Annexin A2 Antibody (3E8-B6)

Western Blot: Annexin A2 Antibody (3E8-B6) [H00000302-M01] - Analysis of ANXA2 expression in transfected 293T cell line by ANXA2 monoclonal antibody (M01), clone 3E8-B6.Lane 1: ANXA2 transfected lysate (Predicted MW: 40.4 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: Annexin A2 Antibody (3E8-B6) [H00000302-M01] - Analysis of monoclonal antibody to ANXA2 on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: Annexin A2 Antibody (3E8-B6) [H00000302-M01] - Analysis of ANXA2 over-expressed 293 cell line, cotransfected with ANXA2 Validated Chimera RNAi ( Cat # H00000302-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ANXA2 monoclonal antibody (M01), clone 3E8-B6 (Cat # H00000302-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: Annexin A2 Antibody (3E8-B6) [H00000302-M01] - ANXA2 monoclonal antibody (M01), clone 3E8-B6 Analysis of ANXA2 expression in Hela.

Product Discontinued

Annexin A2 Antibody (3E8-B6) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ANXA2 (AAH09564.1, 1 a.a. ~ 339 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MFTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETLILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD
• Specificity: ANXA2 - annexin A2
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANXA2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recombinant protein and cell lysate for WB. It has also been used for IF, RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Annexin A2 Antibody (3E8-B6)

• Annexin A2
• Annexin II
• annexin-2
• ANX2
• ANX2L4
• ANX2L4LPC2
• ANXA2
• CAL1H
• Calpactin I heavy chain
• calpactin I heavy polypeptide
• Calpactin-1 heavy chain
• chromobindin 8
• chromobindin-8
• LIP2
• LIP2PAP-IV
• Lipocortin II
• Lipocortin-2
• LPC2D
• LPC2DP36
• p36
• PAP-IV
• Placental anticoagulant protein IV
• Protein I

Background

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Annexin A2 Antibody (H00000302-M01)(2)

Publications using H00000302-M01

• Sun Y, Gao G, Cai J et al. Annexin A2 is a discriminative serological candidate in early hepatocellular carcinoma. Carcinogenesis. 2013-03-01 [PMID: 23188673]
• Deng FY, Lei SF, Zhang Y et al. Peripheral blood monocyte-expressed ANXA2 geneis involved in pathogenesis of osteoporosis in humans. Mol Cell Proteomics. 2011-08-04 [PMID: 21817168]

Bioinformatics

• Gene Symbol: ANXA2
• Entrez:
  • 302(Human)
• OMIM:
  • 151740(Human)
• Uniprot:
  • AAH09564(Human)