AP-2 gamma Antibody [Unconjugated]

Western Blot shows lysates of MCF‑7 human breast cancer cell line, T47D human breast cancer cell line, MDA‑MB‑468 human breast cancer cell line and SK‑BR‑3 human breast cancer cell line. PVDF membrane was probed with 2 µg/ml of Goat Anti-Human AP‑2 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5059) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for AP‑2 gamma at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

AP-2 gamma was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 10 µg/mL Goat Anti-Human AP-2 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5059) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

AP-2 gamma Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human AP-2 gamma
  Leu128-Val223
  Accession # Q92754
• Specificity: Detects human AP-2 gamma in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) AP-2 alpha is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TFAP2C
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AP-2 gamma Antibody [Unconjugated]

• Activating enhancer-binding protein 2 gamma
• AP2 gamma
• AP-2 gamma
• AP2-GAMMA
• ERF1
• estrogen receptor factor 1
• hAP-2g
• TFAP2C
• TFAP2G
• transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma)
• transcription factor AP-2 gamma (activating enhancer-binding protein 2 gamma)
• transcription factor AP-2 gamma
• Transcription factor ERF-1

Background

AP-2 gamma (Activation protein 2 gamma; also ERF-1) is a 50-55 kDa member of the AP-2 transcription factor family. It is essential for kidney development and placentation of the embryo. In the nucleus, it forms homodimers and heterodimers with other AP-2 family members. Elevated AP-2 family members are highly suggestive of neoplasia. Human AP-2 gamma is 450 amino acids in length. It contains a repressor SUMOylation site as Lys10, a Gln/Pro-rich transactivation domain (aa 30‑119) and a helix-span-helix dimerization region (aa 293-424). One potential splice form exists that shows a four aa substitution for the N-terminal 16 aa, followed by a premature truncation after Gly130. Over aa 128-223, human AP-2 gamma shares 91% and 82% aa sequence identity with porcine and mouse AP-2 gamma , respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AP-2 gamma Antibody (AF5059)(11)

Publications using AF5059

• Wang, L;Wang, C;Moriano, JA;Chen, S;Zuo, G;Cebrián-Silla, A;Zhang, S;Mukhtar, T;Wang, S;Song, M;de Oliveira, LG;Bi, Q;Augustin, JJ;Ge, X;Paredes, MF;Huang, EJ;Alvarez-Buylla, A;Duan, X;Li, J;Kriegstein, AR; Molecular and cellular dynamics of the developing human neocortex at single-cell resolution bioRxiv : the preprint server for biology 2024-08-04 [PMID: 39131371] (Immunocytochemistry, Human)
• Weatherbee, BAT;Weberling, A;Gantner, CW;Iwamoto-Stohl, LK;Barnikel, Z;Barrie, A;Campbell, A;Cunningham, P;Drezet, C;Efstathiou, P;Fishel, S;Vindel, SG;Lockwood, M;Oakley, R;Pretty, C;Chowdhury, N;Richardson, L;Mania, A;Weavers, L;Christie, L;Elder, K;Snell, P;Zernicka-Goetz, M; Distinct pathways drive anterior hypoblast specification in the implanting human embryo Nature cell biology 2024-03-01 [PMID: 38443567] (Immunohistochemistry, Human, Mouse)
• Ruan D, Ye Z, Yuan S et al. Human Early Syncytiotrophoblasts Are Highly Susceptible to SARS-CoV-2 Infection Cell Reports Medicine 11/1/2022 [PMID: 36495872]
• M Bao, J Cornwall-S, E Sanchez-Va, DY Chen, J De Jonghe, S Shadkhoo, F Hollfelder, M Thomson, DM Glover, M Zernicka-G Stem cell-derived synthetic embryos self-assemble by exploiting cadherin codes and cortical tension Nature Cell Biology, 2022-09-13;24(9):1341-1349. 2022-09-13 [PMID: 36100738] (IHC, Mouse)
• Yutong Chen, Dylan Siriwardena, Christopher Penfold, Adam Pavlinek, Thorsten E. Boroviak An integrated atlas of human placental development delineates essential regulators of trophoblast stem cells Development 2022-07-01 [PMID: 35792865]
• Sophie Bergmann, Christopher A. Penfold, Erin Slatery, Dylan Siriwardena, Charis Drummer, Stephen Clark et al. Spatial profiling of early primate gastrulation in utero Nature 2022-09-01 [PMID: 35709828]
• Gianluca Amadei, Kasey Y.C. Lau, Joachim De Jonghe, Carlos W. Gantner, Berna Sozen, Christopher Chan et al. Inducible Stem-Cell-Derived Embryos Capture Mouse Morphogenetic Events In Vitro Developmental Cell 2021-02-08 [PMID: 33378662]
• Sozen B, Cox AL, De Jonghe J, et al. Self-Organization of Mouse Stem Cells into an Extended Potential Blastoid Dev. Cell 12/16/2019 [PMID: 31846649]
• MY Turco, L Gardner, RG Kay, RS Hamilton, M Prater, MS Hollinshea, A McWhinnie, L Esposito, R Fernando, H Skelton, F Reimann, FM Gribble, A Sharkey, SGE Marsh, S O'Rahilly, M Hemberger, GJ Burton, A Moffett Trophoblast organoids as a model for maternal-fetal interactions during human placentation Nature, 2018-11-28;0(0):. 2018-11-28 [PMID: 30487605] (IHC, Human)
• Alexander Murray, Arnold R. Sienerth, Myriam Hemberger Plet1 is an epigenetically regulated cell surface protein that provides essential cues to direct trophoblast stem cell differentiation Scientific Reports 2016-04-28 [PMID: 27121762]
• Latos P, Sienerth A, Murray A, Senner C, Muto M, Ikawa M, Oxley D, Burge S, Cox B, Hemberger M Elf5-centered transcription factor hub controls trophoblast stem cell self-renewal and differentiation through stoichiometry-sensitive shifts in target gene networks. Genes Dev, 2015-11-19;29(23):2435-48. 2015-11-19 [PMID: 26584622] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: TFAP2C
• Uniprot:
  • Q92754()