APC Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: APC Antibody [NBP2-15422] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: APC protein stained by APC antibody [C3], C-term diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody NBP2-43837 diluted at 1:1000. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: APC Antibody [NBP2-15422] - Human colon cancer. APC stained by APC antibody [C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, 20 min.

Immunocytochemistry/Immunofluorescence: APC Antibody [NBP2-15422] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: APC Antibody [NBP2-15422] - Rat colon. APC antibody [C3], C-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: APC Antibody [NBP2-15422] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with APC antibody [C3], C-term (NBP2-15422) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

APC Antibody - BSA Free Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human APC. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: APC
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 312 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Porcine (88%), Bovine (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for APC Antibody - BSA Free

• adenomatosis polyposis coli tumor suppressor
• adenomatosis polyposis coli
• adenomatous polyposis coli protein
• adenomatous polyposis coli
• APC
• BTPS2
• Deleted in polyposis 2.5
• DP2
• DP2.5DP2
• DP3
• FPC
• GS
• Min
• Protein APC

Background

This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for APC Antibody (NBP2-15422)(3)

Publications using NBP2-15422

• Pons V, LEvesque P, Plande M, Rivest S Conditional Genetic Deletion of CSF1 Receptor in Microglia Ameliorates the Physiopathology of Alzheimer s Disease Alzheimers Res Ther 2021-01-06 [PMID: 33402196]
• Quach C, Song Y, Guo H, et al. A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice Nat Commun 2019-12-12 [PMID: 31831743] (WB, Mouse)
• Liu S, Tackmann NR, Yang J, Zhang Y. Disruption of the RP-MDM2-p53 pathway accelerates APC loss-induced colorectal tumorigenesis Oncogene 2016-09-12 [PMID: 27617574] (WB, Mouse)

Bioinformatics

• Gene Symbol: APC
• Uniprot:
  • P25054()