APOBEC3G Antibody - BSA Free

Western Blot: APOBEC3G Antibody [NBP1-77206] - Caco-2 cell lysate in the (A), absence and (B) presence of blocking peptide with APOBEC3G antibody at 5 ug/ml. .

Immunohistochemistry: APOBEC3G Antibody - BSA Free [NBP1-77206] - Immunohistochemical staining of human spleen using APOBEC3G antibody at 1 ug/mL.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

APOBEC3G Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a synthetic peptide corresponding to 15 amino acids near the amino-terminus of human APOBEC3G. The immunogen is located within the first 50 amino acids of APOBEC3G. Amino Acid Squence: RNTVERMYRDTFSYN
• Specificity: APOBEC3G antibody will also detect the APOBEC3F isoform.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: APOBEC3G
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1 ug/ml
  • Immunohistochemistry-Paraffin 1 ug/ml
  • Western Blot 5 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for APOBEC3G Antibody - BSA Free

• APOBEC-related cytidine deaminase
• APOBEC-related protein 9
• APOBEC-related protein
• apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G
• ARCD
• ARP-9
• bK150C2.7
• CEM-15
• CEM15ARP9
• dJ494G10.1
• DNA dC->dU-editing enzyme APOBEC-3G
• EC 3.5.4
• EC 3.5.4.-
• EC 3.5.4.5
• FLJ12740DNA dC->dU editing enzyme
• MDS019
• phorbolin-like protein MDS019

Background

The Apoliprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytidine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates. Similar to TRIM5alpha which targets incoming retroviral capsids, APOBEC3 plays a major role in cellular defense against retroviral infection as at least two isoforms, APOBEC3G and to a lesser extent APOBEC3F, can be incorporated HIV-1 virions and induce hypermutation in the newly synthesized viral DNA and thus destabilize the viral genome. This innate mechanism of retroviral resistance is counteracted by the HIV-1 Vif protein by inducing the ubiquitization and degradation of APOBEC3G; a single amino acid substitution (D128K) blocks APOBEC3G depletion without affecting its inhibitory activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for APOBEC3G Antibody (NBP1-77206)(1)

Publication using NBP1-77206

• Fardoos R, Nyquist SK, Asowata OE et al. HIV specific CD8+ TRM-like cells in tonsils express exhaustive signatures in the absence of natural HIV control Frontiers in immunology 2022-10-18 [PMID: 36330531] (MI, Human)

Bioinformatics

• Gene Symbol: APOBEC3G
• Uniprot:
  • NP_068594(Human)