Recombinant Human ApoE4 Protein

SDS-Page: Recombinant Human ApoE4 Protein [NBP1-99634] - ApoE4, human recombinant

Product Discontinued

Recombinant Human ApoE4 Protein Summary

• Description: Recombinant protein containing 300 amino acids for Human ApoE4
  
  Source:E. coli
  Amino Acid Sequence:(P02649)
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab123766)
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: APOE
• Purity: >90%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • SDS-Page
  • Western Blot
• Theoretical MW: 34.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Sterile filtered and lyophilized with no additives
• Concentration: Lyoph
• Purity: >90%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute in dH2O to a concentration of 0.1 - 1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for long-term storage.

Alternate Names for Recombinant Human ApoE4 Protein

• Apo-E
• apolipoprotein E
• apolipoprotein E3
• late onset)
• MGC1571

Background

ApoE belongs to a group of proteins that bind reversibly with lipoprotein and play an important role in lipid metabolism. In addition to facilitating solublization of lipids, these proteins help to maintain the structural integrity of lipoproteins, serve as ligands for lipoprotein receptors, and regulate the activity of enzymes involved in lipid metabolism. Significant quantities of ApoE are produced in liver and brain and to some extent in almost every organ. ApoE is an important constituent of all plasma lipoproteins. It's interaction with specific ApoE receptor enables uptake of chylomicron remnants by liver cells, which is an essential step during normal lipid metabolism. It also binds with the LDL receptor (apo B/E). Defects in ApoE are a cause of hyperlipoproteinemia type III. ApoE exists in three major isoforms; E2, E3, and E4, which differ from one another by a single amino-acid substitution. Individuals heterozygous for the ApoE4 allele are at higher risk of late-onset Alzheimer's disease. Recombinant human ApoE4 is a 34.4 kDa protein containing 300 amino acid residues (Accession No. AAB59397). This protein has a N-terminal His-tag.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: APOE