Apolipoprotein E R2/ApoE R2 Antibody - BSA Free

Western Blot: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2217] - Detection of ApoER2 in mouse brain membrane lysate using NB 100-2217. ECL exposure, 1 min.

Immunocytochemistry/Immunofluorescence: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2217] - Antibody was tested in Neuro-2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

• Reactivity: Hu, Mu, Bv, Ch
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.96 mg/ml

Apolipoprotein E R2/ApoE R2 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to a C-terminal portion of the human ApoER2 protein sequence (between residues 800-900). [UniProt# Q14114]
• Localization: Type I membrane. Isoforms that contain the exon encoding for a furin-cleavage site are proteolytically processed, leading to a secreted receptor fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LRP8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 5 ug/ml
  • Western Blot 2 ug/ml
• Application Notes: In Western blot, a specific band is seen at ~106 kDa position representing the membrane form of ApoER2 and a larger ~130 kDa band may also be seen which represents the glycosylated form of ApoER2.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 0.96 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Apolipoprotein E R2/ApoE R2 Antibody - BSA Free

• ApoER2
• APOER2HSZ75190
• Apolipoprotein E R2
• Apolipoprotein E receptor 2
• low density lipoprotein receptor-related protein 8, apolipoprotein e receptor
• low-density lipoprotein receptor-related protein 8
• Lr8b
• LRP8
• LRP-8
• MCI1

Background

ApoER2 (apolipoprotein E receptor 2 or LDL receptor related protein 8) is a member of LDL receptor (LDLR) family and it acts as a cell surface receptor for Reelin (RELN) and apolipoprotein E (apoE)-containing ligands. ApoER2 facilitates extracellular RELN signal transduction to intracellular signaling processes, mainly through binding with DAB1 on its cytoplasmic tail. RELN acts via both VLDLR and ApoER2 to regulate tyrosine phosphorylation mediated DAB1 activation and microtubule function in neuronal cells. ApoER2 possess higher affinity for RELN compared to VLDLR and is therefore, a key player in Reelin pathway that governs forebrain's neuronal layering during prenatal brain development. ApoER2 associates with ER resident receptor-associated protein (RAP) as well as with dimers of beta 2-glycoprotein I. In the vasculature, ApoER2 has been suggested to implicate in suppressing platelet aggregation. Moreover, ApoER2 is highly expressed in epididymis (initial segment), where it affects the functional expression of clusterin and phospholipid hydroperoxide glutathione peroxidase (PHGPx), which are required for sperm maturation. ApoER2 also function as an endocytic receptor and defective ApoER2 have been linked to myocardial infarction type 1 (MCI1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LRP8