![Immunohistochemistry-Paraffin: Aquaporin-9 Antibody [NBP1-30865] - IHC on paraffin sections of human large intestine tissue. HIER: 1 mM EDTA, pH 8 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/Aquaporin-9-Antibody-Immunohistochemistry-Paraffin-NBP1-30865-img0009.jpg "Immunohistochemistry-Paraffin: Aquaporin-9 Antibody [NBP1-30865] - IHC on paraffin sections of human large intestine tissue. HIER: 1 mM EDTA, pH 8 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Description | The 0.1mg split size of this product is provided in liquid format (reconstituted in sterile water). |
| Immunogen | A synthetic peptide from amino acid region 240-290 of human Aquaporin-9 conjugated to blue carrier protein was used as the antigen. |
| Localization | Cell membrane |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | AQP9 |
| Purity | Unpurified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from whole serum. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Unpurified |
| Reconstitution Instructions | Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format. |
| Publication using NBP1-30865 | Applications | Species |
|---|---|---|
| Ren L, Li P, Li Z, Chen Q AQP9 and ZAP70 as immune-related prognostic biomarkers suppress proliferation, migration and invasion of laryngeal cancer cells BMC cancer 2022-04-28 [PMID: 35477402] (WB, IF/IHC, Human) | WB, IF/IHC | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for Aquaporin-9 Antibody (NBP1-30865)Find related products by research area.
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![Immunohistochemistry-Paraffin: Aquaporin-9 Antibody [NBP1-30865] - IHC on paraffin sections of human stomach tissue. HIER: 1 mM EDTA, pH 8 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/Aquaporin-9-Antibody-Immunohistochemistry-Paraffin-NBP1-30865-img0001.jpg "Immunohistochemistry-Paraffin: Aquaporin-9 Antibody [NBP1-30865] - IHC on paraffin sections of human stomach tissue. HIER: 1 mM EDTA, pH 8 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.")
![Immunohistochemistry-Paraffin: Aquaporin-9 Antibody [NBP1-30865] - IHC on paraffin sections of human stomach tissue. HIER: 1 mM EDTA, pH 8 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/Aquaporin-9-Antibody-Immunohistochemistry-Paraffin-NBP1-30865-img0010.jpg "Immunohistochemistry-Paraffin: Aquaporin-9 Antibody [NBP1-30865] - IHC on paraffin sections of human stomach tissue. HIER: 1 mM EDTA, pH 8 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.")
![Immunohistochemistry-Paraffin: Aquaporin-9 Antibody [NBP1-30865] - IHC on paraffin sections of human stomach tissue. HIER: 1 mM EDTA, pH 8 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/Aquaporin-9-Antibody-Immunohistochemistry-Paraffin-NBP1-30865-img0004.jpg "Immunohistochemistry-Paraffin: Aquaporin-9 Antibody [NBP1-30865] - IHC on paraffin sections of human stomach tissue. HIER: 1 mM EDTA, pH 8 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.")
![Immunohistochemistry-Paraffin: Aquaporin-9 Antibody [NBP1-30865] - IHC on paraffin sections of human testis tissue. HIER: 1 mM EDTA, pH 8 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/Aquaporin-9-Antibody-Immunohistochemistry-Paraffin-NBP1-30865-img0005.jpg "Immunohistochemistry-Paraffin: Aquaporin-9 Antibody [NBP1-30865] - IHC on paraffin sections of human testis tissue. HIER: 1 mM EDTA, pH 8 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.")
![Immunohistochemistry-Paraffin: Aquaporin-9 Antibody [NBP1-30865] - IHC on paraffin sections of human brain tissue. HIER: 1 mM EDTA, pH 8 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/Aquaporin-9-Antibody-Immunohistochemistry-Paraffin-NBP1-30865-img0007.jpg "Immunohistochemistry-Paraffin: Aquaporin-9 Antibody [NBP1-30865] - IHC on paraffin sections of human brain tissue. HIER: 1 mM EDTA, pH 8 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")