Arginase 1/ARG1/liver Arginase Antibody - BSA Free

Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody.

Immunohistochemistry: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - TRP53-regulated EndMT modulates the M1 and M2 populations of increased TAMs after radiotherapy. Immunofluorescence detection of F4/80, CD31, and Arg1, in KP tumours from WT and EC-p53KO mice, with or without irradiation (23 days after irradiation). Image collected and cropped by Citeab from the following publication (Tumour-vasculature development via endothelial-to-mesenchymal transition after radiotherapy controls CD44v6+ cancer cell and macrophage polarization. Nat Commun (2018) licensed under a CC-BY license.

Simple Western: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Simple Western lane view shows a specific band for ARG1 in human and mouse Liver lysate using ARG1 antibody (NBP1-32731) at 25 ug/ml. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Flow Cytometry: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Effects of berberine on the M2 population of macrophages. Representative dot plot of M2 subpopulations in control group and the rats injected with ISO 3 and 7 days later. M2 cells were labelled with F4/80 and Arg1. Image collected and cropped by Citeab from the following publication (Protective role of berberine in isoprenaline-induced cardiac fibrosis in rats. BMC Cardiovasc Disord (2019) licensed under a CC-BY license.

Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Arginase 1 antibody detects Arginase 1 protein. Various whole cell extracts (30ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody diluted at 1:1000.

Immunoprecipitation: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Analysis was performed using Arginase 1 antibody EasyBlot anti-Rabbit IgG was used as a secondary reagent. Arginase 1 protein from HepG2 whole cell extracts using 5 ug of Arginase 1 antibody.

Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody diluted at 1:10000.

Immunocytochemistry/Immunofluorescence: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - An intracellular stain was performed on HepG2 cells with Arginase 1/ARG1/liver Arginase Antibody NBP1-32731AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody diluted at 1:10000.

Immunocytochemistry/Immunofluorescence: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Paraformaldehyde-fixed HeLa.

Immunocytochemistry/Immunofluorescence: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-ARG1 at 10 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Cal27 xenograft, using arginase I antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Tissue section of the mouse liver using 1:200 dilution of ARG1 antibody (NBP1-32731). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.

Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Hepatocellular carcinoma stained with Arginase1 1:100, pH9 antigen retrieval. Image from verified customer review.

Flow Cytometry: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - An intracellular stain was performed on HepG2 with NBP1-32731 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.

Flow Cytometry: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - An intracellular stain was performed on RH30 cells with Arginase 1 Antibody NBP1-32731 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.

Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Western blot analysis and gel protein densitometry were used for quantification of MARCH1 (NBP1-59758) and Arginase 1 (NBP1-32731) proteins on transduced macrophage D: The gel image indicates the blot results 48 and 72 hr after transduction, compared with the non-transduced cells. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35656075/) licensed under a CC-BY license.

Effects of berberine on the M2 population of macrophages. Representative dot plot of M2 subpopulations in control group and the rats injected with ISO 3 and 7 days later. M2 cells were labelled with F4/80 and Arg1. *P < 0.05 as compared with the control group. #P < 0.05 vs. the corresponding ISO group

Immunocytochemistry/ Immunofluorescence: Arginase 1/ARG1/liver Arginase Antibody - BSA Free [NBP1-32731] - Validation of the LCM/MS data from cellular protrusions using microscopy. Immunofluorescence (IF) of proteins identified by LCM/MS (A–D) “expected” or (E–H) “not expected” to be within protrusions based on their known functions/localizations. (A) Cd47 & (B) Anxa2 are found in both hCAD/dCAD protrusions. (C) Tenm2 is found within hCAD protrusions & (D) Cobl in GCs. Interestingly, (E) Grk5 was observed in hCAD protrusions; (F) Hist1h3b protein in hCAD protrusions & GCs (G) Hspa1b in dCAD protrusions & TNTs & (H) Arg1 in dCAD protrusions, GCs, & TNTs. White arrows/arrowheads show punctates within dCAD/hCAD protrusions & GCs or within TNTs, respectively. Scale bars = 10 μm. IF of all 8 proteins corroborate the LCM/MS protein identification for each subtypes of protrusions. (I) 1198 unique proteins were identified by LCM/MS to be in protrusions & 169 were found to be exclusive to protrusions. 904 of the 1198 proteins found in protrusions & 87 out of 169 exclusive proteins had images in the HPA Subcell database. IF images for the proteins identified by LCM/MS in protrusions, present in the HPA Subcell database, were observed & catalogued as being observed or not in protrusions (Figure S2). On average 4.7% of proteins are found in protrusions in the HPA database, 13.6% are found in our LCM isolated protrusions & 23% were found in the “exclusive” LCM protrusions. The fold enrichment increases by 2.9% or 4.8% when we looked at proteins identified by LCM/MS in protrusions or exclusive to protrusions, respectively. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30866487), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Arginase 1/ARG1/liver Arginase was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Rabbit anti-Arginase 1/ARG1/liver Arginase Affinity Purified Polyclonal Antibody conjugated to FITC (Catalog # NBP1-32731F) (green) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (dark blue). Cells were imaged using a 100X objective and digitally deconvolved.

• Reactivity: Hu, Mu, Rt, Bv, Rb
• Applications: WB, Simple Western, Flow, ICC/IF, IHC, IP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Arginase 1/ARG1/liver Arginase Antibody - BSA Free Summary

• Immunogen: Arginase 1/ARG1/liver Arginase Antibody made from full length human Arginase 1 Recombinant protein.
• Localization: Cytoplasm/Nucleus
• Predicted Species: Bovine (90%), Rabbit (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ARG1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 5 - 10 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:10 - 1:500
  • Knockdown Validated
  • Simple Western
  • Western Blot 1:1000-1:50000

Reactivity Notes

Rat reactivity reported in scientific literature (PMID:32701588). Immunogen displays the following percentage of sequence identity for non-tested species: Porcine (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Arginase 1/ARG1/liver Arginase Antibody - BSA Free

• AI
• ARG1
• Arginase 1
• arginase, liver
• Arginase-1
• EC 3.5.3.1
• EC:3.5.3.1
• Liver Arginase
• Liver-type arginase
• PGIF
• Type I Arginase

Background

Arginase 1 (ARG1), also known as liver arginase, is a metalloenzyme that is a member of the ureohydrolase superfamily and arginase family (1). ARG1 is known for its role in the urea cycle in catalyzing the conversion of L-arginine into urea and L-ornithine (1). Arginase has two distinct isoforms, with ARG1 being expressed primarily in the liver and ARG2 in extrahepatic tissues (1). Human ARG1 is synthesized as 322 amino acids (aa) in length with a theoretical molecular weight of 35 kDa (1,2). Three ARG1 monomers can form a highly active homotrimer of 105 kDa (1). A key structural feature of the arginase protein is the binuclear magnesium (Mn2+) ions at its core (1).

Arginase and nitric oxidase synthase (NOS) compete for the same L-arginine substrate, creating a delicate balance between pathways (1). Furthermore, bioavailability of L-arginine and ARG1 expression has been implicated in several pathologies including vascular disease, neuronal disease, cardiovascular disease, immune dysfunction, inflammation, and cancer (1,3-5). For instance, ARG1 functions as a macrophage marker, defining the M2 population, while inducible nitric oxide synthase (iNOS) characterizes the M1 population; impaired M1/M2 polarization and changes in ARG1 expression is observed in diseases such as arteriogenesis, asthma, pulmonary fibrosis, and inflammatory bowel disease (1,3). In humans, arginase deficiency, known as argininemia, is an autosomal recessive metabolic disorder characterized by elevated ammonia (hyperammonemia) levels and arginine accumulation (6). Given that many arginase-associated diseases are characterized by upregulation in expression of ARG1, ARG2, or both, arginase inhibitors are currently being studied as a potential therapeutic approach (1,4).

References

1. S Clemente, G., van Waarde, A., F Antunes, I., Domling, A., & H Elsinga, P. (2020). Arginase as a Potential Biomarker of Disease Progression: A Molecular Imaging Perspective. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms21155291

2. Uniprot (P05089)

3. Kieler, M., Hofmann, M., & Schabbauer, G. (2021). More than just protein building blocks: How amino acids and related metabolic pathways fuel macrophage polarization. The FEBS Journal. Advance online publication. https://doi.org/10.1111/febs.15715

4. Shosha, E., Fouda, A. Y., Narayanan, S. P., Caldwell, R. W., & Caldwell, R. B. (2020). Is the Arginase Pathway a Novel Therapeutic Avenue for Diabetic Retinopathy?. Journal of Clinical Medicine. https://doi.org/10.3390/jcm9020425

5. Correale J. (2021). Immunosuppressive Amino-Acid Catabolizing Enzymes in Multiple Sclerosis. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2020.600428

6. Morales, J. A., & Sticco, K. L. (2020). Arginase Deficiency. In StatPearls. StatPearls Publishing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Arginase 1/ARG1/liver Arginase Antibody (NBP1-32731)(24)

Publications using NBP1-32731

• Seok Han B, Ko S, Seok Park M et Al. Lidocaine combined with general anesthetics impedes metastasis of breast cancer cells via inhibition of TGF-?/Smad-mediated EMT signaling by reprogramming tumor-associated macrophages Int Immunopharmacol 2024-09-22 [PMID: 39312860]
• Sun L, Zhao Z, Guo J et al. Mitochondrial transplantation confers protection against the effects of ischemic stroke by repressing microglial pyroptosis and promoting neurogenesis Neural Regeneration Research 2024-06-01 [PMID: 37905882]
• Chen Z, Yang X, Chen Z et al. A new histone deacetylase inhibitor remodels the tumor microenvironment by deletion of polymorphonuclear myeloid-derived suppressor cells and sensitizes prostate cancer to immunotherapy BMC medicine 2023-10-25 [PMID: 37880708] (Flow Cytometry, Mouse)
• Jiang Y, Liao H, Yan L et al. A Metal-Organic Framework-Incorporated Hydrogel for Delivery of Immunomodulatory Neobavaisoflavone to Promote Cartilage Regeneration in Osteoarthritis ACS applied materials & interfaces 2023-10-11 [PMID: 37769191]
• Sohn HS, Choi JW, Jhun J Et al. Tolerogenic nanoparticles induce type II collagen-specific regulatory T cells and ameliorate osteoarthritis Sci Adv 2022-11-25 [PMID: 36427299] (IF/IHC, Mouse)
  
  Details:
  Citation using the FITC version of this antibody.
• Laplantine E, Chable-Bessia C, Oudin A, Swain J et Al. The FDA-approved drug Auranofin has a dual inhibitory effect on SARS-CoV-2 entry and NF-?B signaling iScience 2022-09-12 [PMID: 36093378]
• Kang R, Gamdzyk M, Luo Y et al. Three Days Delayed Recanalization Improved Neurological Function in pMCAO Rats by Increasing M2 Microglia-Possible Involvement of the IL-4R/STAT6/PPAR gamma Pathway Translational stroke research 2022-07-22 [PMID: 35867328] (WB, Rat)
  
  Details:
  Dilution used 1:1001
• Al-Qazazi R, Lima PDA, Prisco SZ Et al. Macrophage-NLRP3 Activation Promotes Right Ventricle Failure in Pulmonary Arterial Hypertension Am J Respir Crit Care Med 2022-06-14 [PMID: 35699679] (ICC/IF, Rat)
  
  Details:
  Citation using the Alexa Fluor 700 version of this antibody.
• Zangeneh Z, Khamisipour G, Andalib AR Induced overexpression of MARCH-1 in human macrophages altered to M2 phenotype for suppressing inflammation process Iranian journal of basic medical sciences 2022-04-01 [PMID: 35656075] (WB, Human)
• He X, Li X, Zhang M et al. Role of molybdenum in material immunomodulation and periodontal wound healing: Targeting immunometabolism and mitochondrial function for macrophage modulation Biomaterials 2022-03-05 [PMID: 35247634]
• Mou KJ, Shen KF, Li YL et al. Adenosine A2A Receptor in Bone Marrow-Derived Cells Mediated Macrophages M2 Polarization via PPAR gamma-P65 Pathway in Chronic Hypoperfusion Situation Frontiers in aging neuroscience 2022-01-03 [PMID: 35046793] (IHC-P, ICC/IF, Mouse)
• De-Huang Guo, Masaki Yamamoto, Caterina M. Hernandez, Hesam Khodadadi, Babak Baban, Alexis M. Stranahan Beige adipocytes mediate the neuroprotective and anti-inflammatory effects of subcutaneous fat in obese mice Nature Communications 2021-07-30 [PMID: 34330904]
• de Haan W, Dheedene W, Apelt K et Al. Endothelial Zeb2 preserves the hepatic angioarchitecture and protects against liver fibrosis Cardiovasc Res 2022-04-01 [PMID: 33909875]
• Maisonneuve C, Tsang D, Foerster E Et Al. Nod1 Promotes Colorectal Carcinogenesis by Regulating the Immunosuppressive Functions of Tumor-Infiltrating Myeloid Cells Cell Rep 2021-01-27 [PMID: 33503439]
• Chuanqi Cai, Sreenivasulu Kilari, Avishek K. Singh, Chenglei Zhao, Michael L. Simeon, Avanish Misra, Yiqing Li, Sanjay Misra Differences in Transforming Growth Factor‐ beta 1/BMP7 Signaling and Venous Fibrosis Contribute to Female Sex Differences in Arteriovenous Fistulas Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease 2020-08-06 [PMID: 32757791]
• Pan X, Zhang K, Shen C et al. Astaxanthin promotes M2 macrophages and attenuates cardiac remodeling after myocardial infarction by suppression inflammation in rats Chin. Med. J. 2020-08-05 [PMID: 32701588] (FLOW, Rat)
  
  Details:
  Citation using the Allophycocyanin format of this antibody.
• Hozain S, Cottrell J CDllb+ targeted depletion of macrophages negatively affects bone fracture healing Bone 2020-06-12 [PMID: 32535290] (IHC-P, Mouse)
• Cai C, Zhao C, Kilari S et al. Effect of Sex Differences in Treatment Response to Angioplasty in a Murine Arteriovenous Fistula Model Am. J. Physiol. Renal Physiol. 2019-12-09 [PMID: 31813252]
• Che Y, Shen DF, Wang ZP et al. Protective role of berberine in isoprenaline-induced cardiac fibrosis in rats BMC Cardiovasc Disord 2019-10-15 [PMID: 31615408] (FLOW, Rat)
• Gousset K, Gordon A, Kumar Kannan S, Tovar J. A novel Microproteomic Approach Using Laser Capture Microdissection to Study Cellular Protrusions Int J Mol Sci 2019-03-07 [PMID: 30866487] (ICC/IF, Mouse)
• Choi SH, Kim AR, Nam JK, et al. Tumour-vasculature development via endothelial-to-mesenchymal transition after radiotherapy controls CD44v6+ cancer cell and macrophage polarization. Nat Commun. 2018-11-30 [PMID: 30504836] (IF/IHC, WB, Mouse)
• Cao Y, Xu Y, Auchoybur ML et al. Regulatory role of IKKa in myocardial ischemia/reperfusion injury by the determination of M1 versus M2 polarization of macrophages J. Mol. Cell. Cardiol. 2018-08-25 [PMID: 30153439] (ICC/IF, Mouse)
• Yang A, Herter-Sprie G, Zhang H, et al. Autophagy Sustains Pancreatic Cancer Growth through Both Cell-Autonomous and Nonautonomous Mechanisms. Cancer Discov. 2018-01-09 [PMID: 29317452] (IF/IHC, Mouse)
• Yan J, Tie G, Wang S et al. Diabetes impairs wound healing by Dnmt1-dependent dysregulation of hematopoietic stem cells differentiation towards macrophages Nat Commun 2018-01-02 [PMID: 29295997] (IHC-Fr, Mouse)

Review for Arginase 1/ARG1/liver Arginase Antibody (NBP1-32731) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Immunofluorescence - paraffin Human 03/09/2019

Summary

• Application: Other
• Sample Tested: Hepatocellular carcinoma
• Species: Human
• Lot: I don't have it any more

Comments

• Comments: pH9 antigen retrieval

FAQs for Arginase 1/ARG1/liver Arginase Antibody (NBP1-32731). (Showing 1 - 1 of 1 FAQs).

1.  Does the anti-arginase 1 antibody have any specificity towards Arginase II?
   • NBP1-32731 has not been epitope mapped, and cross-reactivity with Arginase II has not been explicitly tested.As you are probably aware, ARG1 and ARG2 share about 60% homology with each other. Since this antibody has not been epitope mapped its hard to say whether this antibody will also detect ARG2. There are some stretches of conservation that are 15 AA long, so it is possible that ARG2 could be recognized with this antibody, especially considering it is a polyclonal antibody. We have 20 primary antibodies that target ARG1, so perhaps you could find one that has the immunogen listed here

Bioinformatics

• Gene Symbol: ARG1
• Entrez:
  • 383(Human)
• Uniprot:
  • P05089()