ARID1A Antibody - BSA Free

Western Blot: ARID1A Antibody [NB100-55334] - Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibody used for WB at 0.1 ug/ml.

Chromatin Immunoprecipitation: ARID1A Antibody [NB100-55334] - In vivo Smarce1-binding sites were detected by chromatin immunoprecipitation using a SMARCE1 antibody. Cropped gel images representing typical results from ChIP assays in: the regulatory module located from the transcription initiation site +1 to -1415, which includes the basic promoter (gata5-Bp), the module located from -1469 to -1892 (gata5-A), the module located from -7823 to -8213 (gata5-B) and the module located from -16805 to -17147 (gata5-C). IgG, Smarce1, Smarca4, Arid1a and Pbrm indicate the PCR products from genomic DNA precipitated using the corresponding antibody. WT and Mut indicate the source of genomic DNA. The input is the PCR product from the total input genomic DNA. Full-length gels are presented in Supplementary Figure 3. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-33746-8), licensed under a CC-BY license.

Western Blot: ARID1A Antibody [NB100-55334] - Mouse embryonic fibroblasts nuclear extract at 1:1000 dilution. (Nuclear extract instead of whole cell lysate provides much better signal.) Image provided via verified customer review.

Western Blot: ARID1A Antibody [NB100-55334] - Analysis of ARID1A in zebrafish embryos using anti-ARID1A antibody. Image from verified customer review.

• Reactivity: Hu, Mu, Ze
• Applications: WB, ChIP, IP (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ARID1A Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 750 and 800 of human AT rich interactive domain 1A (BRG1-associated factor 250kD) using the numbering given in entry NP_006006.3 (GeneID 8289).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ARID1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Western Blot 1:2000-1:10000
• Application Notes: Use in chromatin immunoprecipitation reported in scientific literature (PMID: 30337622). Immunoprecipitation Not recommended. ARID1A antibody validated for WB from verified customer reviews.

Reactivity Notes

Mouse, Zebrafish reactivity reported from verified customer reviews.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ARID1A Antibody - BSA Free

• ARID domain-containing protein 1A
• AT rich interactive domain 1A (SWI- like)
• AT rich interactive domain 1A (SWI-like)
• AT-rich interactive domain-containing protein 1A
• B120SWI-like protein
• BAF250a
• BAF250SWI/SNF complex protein p270
• BM029
• brain protein 120
• BRG1-associated factor 250
• BRG1-associated factor 250a
• C10rf4
• C1orf4SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatinsubfamily F member 1
• chromatin remodeling factor p250
• hELD
• hOSA1
• matrix associated, actin dependent regulator of chromatin
• Osa homolog 1
• OSA1 nuclear protein
• OSA1
• P270
• SMARCF1
• subfamily f, member 1

Background

This gene encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARID1A Antibody (NB100-55334)(5)

Publications using NB100-55334

• Yakovlev VA, Sullivan SA, Fields EC, Temkin SM. PARP inhibitors in the treatment of ARID1A mutant ovarian clear cell cancer: PI3K/Akt1-dependent mechanism of synthetic lethality Frontiers in Oncology 2023-02-22 [PMID: 36910637]
• Shi H, Tao T, Abraham B et al. ARID1A loss in neuroblastoma promotes the adrenergic-to-mesenchymal transition by regulating enhancer-mediated gene expression Sci. Adv. 2020-07-01 [PMID: 32832616] (WB, Zebrafish)
• Bullard WL, Kara M, Gay LA et al. Identification of murine gammaherpesvirus 68 miRNA-mRNA hybrids reveals miRNA target conservation among gammaherpesviruses including host translation and protein modification machinery PLoS Pathog. 2019-08-01 [PMID: 31393953] (WB, Mouse)
• Castillo-Robles J, Ramirez L, Spaink HP, Lomeli H. smarce1 mutants have a defective endocardium and an increased expression of cardiac transcription factors in zebrafish. Sci Rep. 2018-10-18 [PMID: 30337622] (Chemotaxis, Zebrafish)
• Wurster AL, Precht P, Pazin MJ et al. NF-?B and BRG1 bind a distal regulatory element in the IL-3/GM-CSF locus. Mol Immunol 2011-09-01 [PMID: 21831442]

Reviews for ARID1A Antibody (NB100-55334) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Mouse, Other.

reviewed by: Verified Customer WB Other 03/23/2015

Summary

• Application: Western Blot
• Sample Tested: zebrafish embryos
• Species: Other

reviewed by: Xiaofeng Wang WB Mouse 02/28/2014

Summary

• Application: Western Blot
• Sample Tested: mouse embryonic fibroblasts nuclear extract
• Species: Mouse
• Lot: 1

Bioinformatics

• Gene Symbol: ARID1A
• Entrez:
  • 8289(Human)
• Uniprot:
  • O14497(Human)