ARPC4 Antibody - BSA Free

Western Blot: ARPC4 Antibody [NBP1-69003] - Mouse Intestine, concentration 0.2-1 ug/ml.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

ARPC4 Antibody - BSA Free Summary

• Description: The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
• Immunogen: Synthetic peptides corresponding to Arpc4 (actin related protein 2/3 complex, subunit 4) The peptide sequence was selected from the N terminal of Arpc4. Peptide sequence AVRATLQAALCLENFSSQVVERHNKPEVEVRSSKELLLQPVTISRNEKEK. The peptide sequence for this immunogen was taken from within the described region.
• Specificity: This product is specific to Subunit or Isoform: 4.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ARPC4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1.0 ug/ml
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 2% Sucrose
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for ARPC4 Antibody - BSA Free

• actin related protein 2/3 complex, subunit 4, 20kDa
• actin-related protein 2/3 complex subunit 4
• arp2/3 protein complex subunit p20
• MGC13544
• p20-Arc
• subunit 4 (20 kD)

Background

Arpc4 functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARPC4 Antibody (NBP1-69003)(2)

Publications using NBP1-69003

• Sadhu L, Tsopoulidis N, Hasanuzzaman M et al. ARPC5 isoforms and their regulation by calcium-calmodulin-N-WASP drive distinct Arp2/3-dependent actin remodeling events in CD4 T cells eLife 2023-05-10 [PMID: 37162507] (Western Blot)
• Schrank BR, Aparicio T, Li Y et al. Nuclear ARP2/3 drives DNA break clustering for homology-directed repair Nature 2018-06-20 [PMID: 29925947] (WB)

Reviews for ARPC4 Antibody (NBP1-69003) (2)

Average Rating: 3.5

(Based on 2 reviews)

We have 2 review tested in 1 species: Other.

reviewed by: Ben Schrank WB Other 08/20/2014

Summary

• Application: Western Blot
• Species: Other

reviewed by: Benjamin Schrank WB 08/20/2014

Summary

• Application: Western Blot
• Lot: NBP1-69003

Bioinformatics

• Gene Symbol: ARPC4
• Uniprot:
  • P59999(Mouse)