ATF6 Antibody (70B1413.1) - BSA Free

Western Blot: ATF6 Antibody (70B1413.1) [NBP1-40256] - Hypoxia leads to EMT and ER-stress in CRC cells. B. C. Confluent growing SW480 (B) and HCT116 (C) cells were cultured under conditions of normoxia or hypoxia-like conditions (serum free; 100 uM CoCl2, 1-9 h. B-actin (B-act) as loading control. HIF1a was detectable after 3 h of CoCl2 incubation, the amount of the 50 kD-ATF6 fragment, was already enhanced after 1 h of addition of CoCl2. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0087386) licensed under a CC-BY license.

Western Blot: ATF6 Antibody (70B1413.1) [NBP1-40256] - Analysis of ATF6 in mouse liver tissue using 3 ug/ml of ATF6 antibody and 0.25 ug/ml of GAPDH antibody. Lane A contains 20 ugs of whole mouse liver lysate, lane B contains 20 ugs of total ER fraction, and lane C contains 20 ugs of rough ER fraction. The ATF6 band may represent under glycosylated or cleaved/active ATF6.

Immunocytochemistry/Immunofluorescence: ATF6 Antibody (70B1413.1) [NBP1-40256] - Untreated HeLa cells were fixed in -20C methanol for 10 min, air dried and rehydrated in PBS at room temperature for 5 minutes. Cells were incubated with anti-ATF6 (1:20) for one hour at room temperature. ATF6 reactivity (green) was detected with anti-mouse Dylight-488 secondary antibody. Nuclei were counterstained with DAPI (blue). Note the ER localization of ATF6.

Immunohistochemistry-Paraffin: ATF6 Antibody (70B1413.1) [NBP1-40256] - Analysis of ATF6 antibody on Human placenta. Fixed paraffin-embedded sections. Antibody dilution 1:50. Incubated overnight in 4C. Image from verified customer review.

Flow (Intracellular): ATF6 Antibody (70B1413.1) [NBP1-40256] - An intracellular stain was performed on HeLa cells with ATF6 Antibody (70B1413.1) NBP1-40256F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.. Using the FITC format of this antibody.

Western Blot: ATF6 Antibody (70B1413.1) [NBP1-40256] - Lane 1: 293 cells transfected with full-length ATF6. Lane 2: 293 cells transfected with partial length ATF6 (amino acids 1-373). Lane 3: Untransfected 293 cells. Western blots were probed with 4 ug/ml of the ATF6 monoclonal antibody and visualized with PicoTect Western Blot Chemiluminescence Substrate (10087K). Film was exposed for 1 min. The top arrow corresponds to the 90 kDa form of ATF6 described as full-length in the literature. The human full-length and partial length ATF6 plasmids are described in Luo and Lee (2002).

Immunohistochemistry-Paraffin: ATF6 Antibody (70B1413.1) [NBP1-40256] - Human placenta, followed by biotinylated horse anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Dilution 10ug/ml

Flow Cytometry: ATF6 Antibody (70B1413.1) [NBP1-40256] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using ATF6 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.

Western Blot: ATF6 Antibody (70B1413.1) [NBP1-40256] - Lysate of DU145 cells. Image from verified customer review.

Western Blot: ATF6 Antibody (70B1413.1) [NBP1-40256] - Lysate of PC-3 cells. Image from verified customer review.

Expression of ER stress-associated transcription factors in NASH, NAFL and normal liver tissues beta -Actin was used as an internal control. Horizontal lines represent means of densitometry signals from the western blot analyses for all tissue groups. #, significant differences in signals between NASH and normal liver tissues (P < 0.05). Data for NAFL tissues were not used for statistical comparisons because of limited sample number (n = 2).

Abnormal Paneth cells show ER stress.(A, B) Representative transmission electron microscopy images of Paneth cells at the base of ileal crypts in (A) ICR & (B) SAMP1/YitFc mice. Scale bars indicate 2 μm. (C, D) Quantitative analysis of (C) granule number & (D) ER lumen diameter in Paneth cells (n = 3/each week for SAMP1/YitFc mice). For the measurements, three Paneth cells were randomly selected from each mouse. (E) SDS–PAGE Western blot analysis of ER stress markers, pIRE1 alpha , ATF4, cleaved-ATF6, & GRP78 in ileal crypts (n = 4/each group). Total-IRE1 alpha & HPRT1 was used as loading control. (F) Relative expression level of ER stress markers calculated from the band intensity. Error bars represent mean ± SEM. (C, D, F) Statistical significance was evaluated by t test in (C, D), & one-way ANOVA followed by Tukey’s post hoc test in (F). P < 0.05 was considered statistically significant. *P < 0.05, †P < 0.01, §P < 0.001. E, ER; G, granules; N, nucleus; n.s., not significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32345659), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ATF6 Antibody (70B1413.1) - BSA Free [NBP1-40256] - The ISR is active in the retinas of rd16 mice.Western blots & corresponding graphs of ISR markers in the retinas of rd16 mice at P15 (a) & P20 (b). At P15, p-eIF2 alpha , ATF4, CHOP, & TRB3 are significantly elevated. At P20, levels of p-eIF2 alpha , ATF4, CHOP, & GADD34 are increased. Markers of the UPR are elevated in rd16 retinas. BiP & cleaved (~50 kD) ATF6 are upregulated in the retinas of rd16 mice at P15 (c) & P20 (d). Relative density measurements correspond to the intensities of the immunoblotting bands or lanes normalized to an internal control. Data are shown as mean ± SEM. a.u. arbitrary units. P15 n = 3. P20 n = 4. *p < 0.05, **p < 0.01, ***p < 0.001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29706649), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ATF6 Antibody (70B1413.1) - BSA Free [NBP1-40256] - Expression of ER stress-associated transcription factors in NASH, NAFL & normal liver tissues beta -Actin was used as an internal control. Horizontal lines represent means of densitometry signals from the western blot analyses for all tissue groups. #, significant differences in signals between NASH & normal liver tissues (P < 0.05). Data for NAFL tissues were not used for statistical comparisons because of limited sample number (n = 2). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28968997), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ATF6 Antibody (70B1413.1) - BSA Free [NBP1-40256] - Restoration of Plk3 induction rescues ATF2 phosphorylation & ER stress resolution in Lrh-1LKO mice, & loss or gain of ATF2 transcriptional activity also alters ER stress resolution capacity.(A) Primary hepatocytes prepared from Lrh-1f/fand Lrh-1LKO mice & transduced with Ad-Plk3 or Ad-control at a MOI of 100. Cells treated 36 hr later with vehicle or tunicamycin (TM) (0.01 µg/ml) & doxycycline (1 µg/ml) to induce Plk3 or LacZ control. Nuclear protein was obtained at timepoints indicated & immunoblotted for UPR transcription factors, with TBP as a loading control. Results representative of 3 independent experiments. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/01694), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ATF6 Antibody (70B1413.1) - BSA Free [NBP1-40256] - The ISR is active in the retinas of rd16 mice.Western blots & corresponding graphs of ISR markers in the retinas of rd16 mice at P15 (a) & P20 (b). At P15, p-eIF2 alpha , ATF4, CHOP, & TRB3 are significantly elevated. At P20, levels of p-eIF2 alpha , ATF4, CHOP, & GADD34 are increased. Markers of the UPR are elevated in rd16 retinas. BiP & cleaved (~50 kD) ATF6 are upregulated in the retinas of rd16 mice at P15 (c) & P20 (d). Relative density measurements correspond to the intensities of the immunoblotting bands or lanes normalized to an internal control. Data are shown as mean ± SEM. a.u. arbitrary units. P15 n = 3. P20 n = 4. *p < 0.05, **p < 0.01, ***p < 0.001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29706649), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ATF6 Antibody (70B1413.1) - BSA Free [NBP1-40256] - APPswe induction of OB-senescence via ER stress.a Heat map of differentially expressed ER stress or anti-stress related genes identified by RNA-seq in control (OCN-Cre; Ai9) & TgAPPsweOCN; Ai9 Td+ OB-progenitors (detail analysis was described in Methods). b RT-PCR analysis of ER stress-related genes Grp78, Atf6, Hsp90b1, Eif2ak3, Ern1, Hsp90aa1, & Hspa2 & anti-stress related gene Sirt3 gene expression in purified Td+ BMSCs from 6-MO control (OCN-Cre; Ai9) & TgAPPsweOCN; Ai9 mice, *p < 0.05, **p < 0.01, ***p < 0.001, mean ± SD, n = 3, Mann–Whitney U test. c Western blot analysis of indicated protein expression in BMSCs from mice with indicated genotypes (at 6-MO). GAPDH was used as a loading control. d Quantification of data in c, *p < 0.05, **p < 0.01. mean ± SD, n = 4, Student’s t test. e Western blot analysis of indicated protein expression in BMSCs from 6-MO control & TgAPPsweOCN with or without 0.25 mM 4-PBA (4-Phenylbutyric acid) treatment. f Quantification analyses of the data in e, *p < 0.05, n = 3. g SA-beta -gal staining of 6-MO control & TgAPPsweOCN BMSCs with vehicle (Veh)(PBS) & 4-PBA treatment, respectively, scale bar, 20 µm. h Quantification of SA-beta -gal+ cell densities in g (mean ± SD; n = 5, **p < 0.01, ***p < 0.001). Two-way analysis of variance test was used in f & h. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34824365), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ATF6 Antibody (70B1413.1) - BSA Free [NBP1-40256] - Disturbance of ER homeostasis in ADTKD-UMOD.(A) ADTKD-UMOD is characterized by maturation & trafficking defect of mutant UMOD & intracellular accumulation of UMOD in TAL cells. UMOD immunolocalization revealed a diffuse cytoplasmic staining with enforcement of the luminal membrane in TAL cells of a wild-type mouse. In contrast, TAL cells of an UmodC93F mutant mouse displayed a strong paranuclear immunopositivity for UMOD. Wild-type: Umodwt mouse; UmodC93F: homozygous UmodC93F mutant mouse. Age of mice analysed: four months. Chromogen: DAB, nuclear staining: haemalum. (B) Heat map of relative expression values (z scores) showed differential abundance of several proteins localized in the ER. (C) In the outer medulla of Umod mutant mice of both mouse lines, a strong accumulation of immature UMOD was present. (D) Protein abundances of BiP, phospho-eIF2 alpha , eIF2 alpha , ATF4, both full-length (§) & cleaved activated (#) ATF6, & CHOP were increased in Umod mutant mice compared to wild-type mice. Signal intensities were corrected for GAPDH signal intensities of the same PVDF-membrane, which was stripped several times to facilitate the detection of multiple proteins. Mean of protein abundance of wild-type mice was set on a value of 1 [mean (wild-type) = 1]. Data are shown as means ± SD. One-way ANOVA with Newman-Keuls’s post hoc test: p vs. wild-type, *p < 0.05; **p < 0.01; ***p < 0.001. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep42970), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ATF6 Antibody (70B1413.1) - BSA Free [NBP1-40256] - The effect of Ezetimibe on unfolded protein response (UPR) gene expression in THP-1 cells exposed to ischemia-reperfusion (IR). (a) The mRNA expression of activating transcription factor 6 (ATF6) & CCAAT-enhancer-binding protein homologous protein (CHOP). (b) Representative Western blot analyses for the indicated proteins. (c,d) The average quantification of ATF6 & CHOP obtained by the densitometric analysis of three independent experiments. mRNA was analyzed by quantitative real-time PCR; normalized gene expression levels are given as the ratio between the mean value for the target gene & that for beta -actin in each sample. Data are expressed as mean ± SD. * p < 0.01 vs. control (up-regulation); ** p < 0.01 vs. IR; §p < 0.01 vs. control (down-regulation). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32340270), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ATF6 Antibody (70B1413.1) - BSA Free [NBP1-40256] - APPswe induction of OB-senescence via ER stress.a Heat map of differentially expressed ER stress or anti-stress related genes identified by RNA-seq in control (OCN-Cre; Ai9) & TgAPPsweOCN; Ai9 Td+ OB-progenitors (detail analysis was described in Methods). b RT-PCR analysis of ER stress-related genes Grp78, Atf6, Hsp90b1, Eif2ak3, Ern1, Hsp90aa1, & Hspa2 & anti-stress related gene Sirt3 gene expression in purified Td+ BMSCs from 6-MO control (OCN-Cre; Ai9) & TgAPPsweOCN; Ai9 mice, *p < 0.05, **p < 0.01, ***p < 0.001, mean ± SD, n = 3, Mann–Whitney U test. c Western blot analysis of indicated protein expression in BMSCs from mice with indicated genotypes (at 6-MO). GAPDH was used as a loading control. d Quantification of data in c, *p < 0.05, **p < 0.01. mean ± SD, n = 4, Student’s t test. e Western blot analysis of indicated protein expression in BMSCs from 6-MO control & TgAPPsweOCN with or without 0.25 mM 4-PBA (4-Phenylbutyric acid) treatment. f Quantification analyses of the data in e, *p < 0.05, n = 3. g SA-beta -gal staining of 6-MO control & TgAPPsweOCN BMSCs with vehicle (Veh)(PBS) & 4-PBA treatment, respectively, scale bar, 20 µm. h Quantification of SA-beta -gal+ cell densities in g (mean ± SD; n = 5, **p < 0.01, ***p < 0.001). Two-way analysis of variance test was used in f & h. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34824365), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: ATF6 Antibody (70B1413.1) - BSA Free [NBP1-40256] - Effect of rapamycin on markers of the UPR pathways(A) Cells were incubated in medium alone (0.05% FBS), with 3 nM IGF-1 or with both 3 nM IGF-1 & 10 nM rapamycin (IGF-1 + rapamycin). Total protein extracts prepared from cells incubated for 24 h in those conditions were subjected to Western Blot analysis. Protein expression levels were assessed for phosphorylated or total forms of PERK, eIF2 alpha & for CHOP, BiP, XBP1-s & XBP1-u proteins. Efficiency of rapamycin to inhibit mTORC1 pathway was also checked by immunoblot with phosphorylated & total forms of p70S6K1 & 4E-BP1. alpha -tubulin was used as internal control. (B & C) Cells were incubated with 10nM rapamycin for 1 h (B) or 24 h (C). Immunoblots for phosphorylated & total forms of PERK, eIF2 alpha , p70S6K1 & 4E-BP1 or for ATF4, CHOP, BiP, XBP1-s & XBP1-u protein expression were performed. alpha -tubulin was used as internal control. Blots of P-p70S6K1, p70S6K1, P-PERK, PERK, & alpha -tubulin of Figure 4B & blots of Figure 4C have been performed on the same electrophoresis gel, but cut & reconstituted. (D) Cells were incubated in medium alone (Ctrl) or with 10 nM rapamycin for 24 h. Nuclear localization of ATF6 was assessed using immunofluorescence with ATF6-antibody (red) & Hoechst dye. Magnification ×1000. (E) Bar graphs were obtained by quantification of ATF6 nuclear staining. Results are representative of at least 3 experiments. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.15469), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: ATF6 Antibody (70B1413.1) - BSA Free [NBP1-40256] - Activation of the UPR during hypoxia or glucose depletionSTC-1 & GluTag cells were subjected to hypoxia (1%) or cultivated with decreasing concentration of glucose i.e. 25, 5 or 1 mM, for 24 h. (A) & (C) Protein expression level of phosphorylated & total forms of PERK, eIF2 alpha & ATF4, CHOP, BiP & C-Caspase 3 protein expression was examined using immunoblots, during hypoxia (A) & glucose depletion (C). alpha -tubulin was used as internal control. Blots of Figure 2A have been performed on the same electrophoresis gel, but cut & reconstituted. (B) & (D) XBP1 mRNA splicing was analyzed by RT-PCR after Pst1 digestion: XBP1-u, unspliced; XBP1-h, hybrid; XBP1-s, spliced variant of XBP1; *, XBP1-u mRNA fragments after Pst1 digestion. (E) ATF6 nuclear localization was assessed in STC-1 cells using immunofluorescence with anti-ATF6 antibody & hoechst dye. Magnification x1000. Results are presentative of at least 3 independent experiments (A–E). (F) Bar graphs were obtained by quantification of ATF6 nuclear staining (*P < 0.05 versus control). Results are presentative of 3 independent experiments (A–E) or the mean ± S.E.M. of an experimental n = 3 (F). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.15469), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: ATF6 Antibody (70B1413.1) - BSA Free [NBP1-40256] - UPR status in STC-1 & GluTag cell lines & effect of UPR inducers on markers of the UPR pathwaysSTC-1 & GluTag cells were incubated in medium (Ctrl) or ER stress-inducing agents thapsigargin (Tg, 300 nM), tunicamycin (Tn, 0.05 μg/mL) & brefeldin A (Bref A, 3 μM) for 4 h, 16 h & 8 h respectively. (A) Protein expression level of phosphorylated or total forms of PERK, eIF2 alpha & CHOP, BiP & cleaved-caspase 3 (C-Caspase 3) protein expression was examined using Western Blot analysis. alpha -tubulin was used as internal control (B) Densitometric quantification of P-PERK/PERK, P-eIF2 alpha /eIF2 alpha & BiP/ alpha -tubulin ratios analysis in STC-1 or GluTag cell lines (*P < 0.05 versus control). (C–D) The effect of Tg, Tn & Bref A on ATF6 nuclear localization was assessed by immunofluorescence in STC-1 cells (C) & GluTag cells (D) using anti-ATF6 antibody & Hoechst dye. Magnification ×1000. (E) Bar graphs obtained by quantification of ATF6 nuclear staining (*P < 0.05 versus control). (F) XBP1 mRNA splicing was analyzed by RT-PCR after Pst1 digestion: XBP1-h, hybrid, XBP1-u, unspliced; XBP1-s, spliced variant of XBP1; *, XBP1-u mRNA fragments after Pst1 digestion. Results are presentative of 3 independent experiments (A, C, D, F) or the mean ± S.E.M. of an experimental n = 3 (B, E). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.15469), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ATF6 Antibody (70B1413.1) - BSA Free [NBP1-40256] - Tm‐induced UPR increases expression of RANKL in cultured osteoblastic & osteocytic cells. (A) Western blotting of cell lysates obtained from neonatal calvaria‐derived osteoblastic cells (Calvaria Ob) treated with 2.2 µg/mL Tm for the indicated times. (B‐D) Gene expression as determined by qRT‐PCR in (B) calvaria‐derived osteoblastic cells (n = 3/group) (C) Osteoblastic UAMS‐32 cells (UAMS‐32 Ob) (n = 4/group) or (D) osteocytic MLO‐Y4 cells (MLO‐Y4 Ot) (n = 4/group),maintained in presence of vehicle (, 0.1% DMSO) or 2.2 µg/ml Tm () for 4 hours. (E) Western blot of RANKL protein in cell lysates obtained from calvaria‐derived osteoblastic cells as described in A in a separate study. Data shown are the mean & SD with individual data points. *P < .05 vs vehicle by Student's t‐test Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32259048), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/Immunofluorescence: Mouse Monoclonal ATF6 Antibody (70B1413.1) [IMGENEX: IMG-273] [NBP1-40256] - Mice hepatocytes were stained for ATF6. Image from a verified customer review.

Western Blot: Mouse Monoclonal ATF6 Antibody (70B1413.1) [IMGENEX: IMG-273] [NBP1-40256] - Western blot of the mouse liver homogenates from control (1) and alcohol-fed mice (2). Image from a verified customer review.

• Reactivity: Hu, Mu, Rt, Po, Pl, Rb
• Applications: WB, Simple Western, Flow, ICC/IF, IHC, IP, WB, ChIP, CyTOF-ready, KO
• Clone: 70B1413.1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ATF6 Antibody (70B1413.1) - BSA Free Summary

• Description: (1) The ATF6 (IMG-273) has been cited to recognize both full-length and cleaved forms of ATF6. Please refer to the references in the Product Citation list for more comprehensive information.
  
  (2) The ATF6 transfected cell lysate (40210) is recommended as a useful western blot positive control to run in parallel with your experimental samples.
  
  (3) Active/cleaved forms of ATF6 are generated through proteolytic cleavage during ER stress. Different molecular weights have been described for cleaved forms. A sample protocol was first described in Luo and Lee (2002) wherein NIH3T3 cells were treated with the amino acid analogue azetidine (AzC), and full-length and cleaved forms ATF6 were detected with IMG-273. The results show that in addition to the major 90 kDa full length ATF6, protein bands over the range of 50-70 kDa were detectable following AzC treatment (Luo and Lee, 2002: Figure 4A, page 791). Many protocols and other cleaved forms have been described since, please consult the literature for additional information.
  
  (4) For immunofluorescence microscopy, cells were fixed in methanol at -20 degrees C (Thomas et al, 2005). Thomas et al used immunofluorescence to identify ATF6 with IMG-273 in the nucleus.
  
  (5) The active/cleaved 50 kDa nuclear form of ATF6 using IMG-273 has been found to be strongly expressed in certain tumor cell lines derived from B cell lymphoma (DEL), primary effusion lymphoma [BC-3 (ATCC CRL-2277), PEL-SY, HBL-6], lymphoblastic leukemia (DS-1) and multiple myeloma (RPMI-8226, NCI-H929), (Jenner et al. 2003).
  
  (6) Cleaved 60 and 36 kDa ATF6 forms have also been described in the nucleus (Mao et al, 2007).
  
  (7) The ATF6 antibody is reported to be specific for ATF6a, recognizing ATF6a but not ATF6b (Bommiasamy et al, 2009).
  
  (8) In western blots, the binding pattern of ATF6 may vary. Researchers are encouraged to consult the body of literature citing the ATF6 IMG-273 antibody (see Product citation list) for additional information. General ATF6 literature is also helpful. For example, Yoshida (1998) show a multiple band pattern in HeLa in both untreated and stressed cells (Fig 11B). In this early landmark ATF6 publication, multiple bands were seen between the 66 and 116 kDa markers as well as one or more bands between the 45 and 66 kDa markers.
  
  (9) We highly recommend the use of a maximum sensitivity ECL substrate (Femto sensitive) for efficient detection of this antibody in Western blot applications.
• Immunogen: This monoclonal antibody was made against a partial protein containing amino acids 1-273 of human ATF6.
• Specificity: This ATF6 antibody detects both the full length and the cleaved/active protein.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ATF6
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry 1 ug per million cells
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
  • Immunoprecipitation 1:10-1:500
  • Knockout Validated
  • Simple Western
  • Western Blot 1-5 ug/ml
• Application Notes: ICC: See Thomas et al (2005) and Kikuchi et al (2006) for details. Immunohistochemistry (Frozen): See Zhu et al (2008) for details. Immunohistochemistry (Paraffin): See van Kollenburg et al (2006) for details. Immunoprecipitation: See Hong et al, 2004 for details. Use in Immunoblotting reported in scientific literature (PMID 28550308). Knockout validation (PMID: 31531232). In Western blot do not use milk in the diluent as it can inhibit the observed signal. This antibody is CyTOF ready.
• Control:

  HeLa DTT Treated / Untreated Cell Lysate

Reactivity Notes

Plant reactivity reported in scientific literature (PMID: 31531232). Use in Plant reported in scientific literature (PMID:31531232).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for ATF6 Antibody (70B1413.1) - BSA Free

• ACHM7
• Activating transcription factor 6 alpha
• activating transcription factor 6
• atf6 a
• ATF6 alpha
• ATF6
• ATF6A
• ATF6-alpha
• cAMP-dependent transcription factor ATF-6 alpha
• cyclic AMP-dependent transcription factor ATF-6 alpha

Background

ATF6 is a constitutively expressed, endoplasmic reticulum (ER) membrane-anchored transcription factor. ATF6 is a key transcriptional activator of the unfolded protein response (UPR), which allows mammalian cells to maintain cellular homeostasis when they are subjected to environmental and physiological stresses that target the ER (reviewed in Shen, 2005 & Prywes, 2005). The C-terminus of ATF6 is located in the ER lumen and its N-terminal DNA binding domain faces the cytosol. AFT6 plays a key role in the ER stress response by transmitting the ER stress signal across the ER membrane into the nucleus. The induction of new gene expression by ATF6 is an important aspect of the ER stress response. In response to certain stress conditions, ATF6 translocates from the ER to the Golgi. The 90 kDa full-length ATF6 is processed within the Golgi to its active 50 kDa form through sequential cleavage by site-1 and site-2 proteases (S1P and S2P). Proteolytic activation of ATF6 in the ER stress response is a mechanism to regulate membrane-bound factors, and is referred to as regulated intramembrane proteolysis. The N-terminal active ATF6 translocates to the nucleus where it binds to ER stress-response elements in ER stress-response genes (ERSRGs). ATF6 is a potent transcriptional activator of ERSRGs. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in denaturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ATF6 Antibody (NBP1-40256)(246)

Publications using NBP1-40256

• Nancy Ahuja, Shalini Gupta, Rashmi Arora, Ella Bhagyaraj, Drishti Tiwari, Sumit Kumar, Pawan Gupta Nr1h4 and Thrb ameliorate ER stress and provide protection in the MPTP mouse model of Parkinson’s Life Science Alliance 2024-04-12 [PMID: 38609183]
• Ryan D.R. Brown, Christopher D. Green, Cynthia Weigel, Bin Ni, Francesco S. Celi, Richard L. Proia, Sarah Spiegel Overexpression of ORMDL3 confers sexual dimorphism in diet-induced non-alcoholic steatohepatitis Molecular Metabolism 2023-12-09 [PMID: 38081412]
• Suravi Majumder, Abhijnan Chattopadhyay, Jamie M Wright, Pujun Guan, L Maximilian Buja, Callie S Kwartler, Dianna M Milewicz Pericentrin deficiency in smooth muscle cells augments atherosclerosis through HSF1-driven cholesterol biosynthesis and PERK activation. JCI insight 2023-11-14 [PMID: 37937642]
• Alexandru P, Chiritoiu G, Lixandru D et al. EDEM1 regulates the insulin mRNA level by inhibiting the endoplasmic reticulum stress-induced IRE1/JNK/c-Jun pathway iScience 2023-09-01 [PMID: 37822496] (WB, Rat)
• Sakuma I, Nagano H, Hashimoto N et al. Identification of genotype-biochemical phenotype correlations associated with fructose 1,6-bisphosphatase deficiency Communications biology 2023-07-28 [PMID: 37507476] (WB)
• Ming-Hong Sun, Wen-Jie Jiang, Xiao-Han Li, Song-Hee Lee, Geun Heo, Dongjie Zhou, Zhi Chen, Xiang-Shun Cui ATF6 aggravates apoptosis in early porcine embryonic development by regulating organelle homeostasis under high-temperature conditions Zoological Research 2023-09-18 [PMID: 37501400]
• Espina M, Di Franco N, Brañas-Navarro M et al. The GRP78-PERK axis contributes to memory and synaptic impairments in Huntington's disease R6/1 mice Neurobiology of disease 2023-07-11 [PMID: 37442396] (WB, Mouse)
  
  Details:
  Dilution: 1:1000
• Li J, Li S, Yu S et al. Persistent ER stress causes GPI anchor deficit to convert a GPI-anchored prion protein (PrP) into pro-PrP via the ATF6-miR449c-5p-PIGV axis The Journal of biological chemistry 2023-06-28 [PMID: 37390992] (IHC-P, Human)
• Kar A, Jain D, Kumar S et al. A localized hydrogel-mediated chemotherapy causes immunogenic cell death via activation of ceramide-mediated unfolded protein response Science advances 2023-06-30 [PMID: 37390205] (WB, Mouse)
• Navas-Madro�al M, Almendra-Pegueros R, Puertas-Umbert L et al. Targeting mitochondrial stress with SS31 prevents experimental abdominal aortic aneurysm: crosstalk with ER stress British journal of pharmacology 2023-03-25 [PMID: 36964990] (WB, Mouse)
• Xiao J, Huang J, Jian X et al. IRE1? arm of unfolded protein response in muscle-specific TGF-? signaling-mediated regulation of muscle cell immunological properties Cellular & molecular biology letters 2023-02-27 [PMID: 36849929] (WB, IHC, Mouse)
• Preston AJ, Rogers A, Sharp M et al. Elephant TP53-RETROGENE 9 induces transcription-independent apoptosis at the mitochondria Cell death discovery 2023-02-16 [PMID: 36797268] (Western Blot)
• Griffin H, Sullivan S, Barger S et al. Liraglutide Counteracts Endoplasmic Reticulum Stress in Palmitate-Treated Hypothalamic Neurons without Restoring Mitochondrial Homeostasis International Journal of Molecular Sciences 2022-12-30 [PMID: 36614074] (ICC/IF, Mouse)
• Cervia L, Shibue T, Borah A et al. Data from A Ubiquitination Cascade Regulating the Integrated Stress Response and Survival in Carcinomas American Association for Cancer Research 2023-04-04 [PMID: 36576405] (Western Blot)
• Cicalese S, Torimoto K, Okuno K et al. Endoplasmic Reticulum Chemical Chaperone 3-Hydroxy-2-Naphthoic Acid Reduces Angiotensin II-Induced Vascular Remodeling and Hypertension In Vivo and Protein Synthesis In Vitro Journal of the American Heart Association 2022-11-29 [PMID: 36444851] (WB, Rat)
  
  Details:
  Dilution: 1:500
• Li Y, Chen L, Li L et al. The Cholesterol-Binding Translocator Protein (18 kDa) Regulates Hepatic Steatosis and Bile Acid Synthesis in Non-Alcoholic Fatty Liver Disease SSRN Electronic Journal 2020-12-22
• Byun JH, Lebeau PF, Platko K et al. Inhibitory Antibodies against PCSK9 Reduce Surface CD36 and Mitigate Diet-Induced Renal Lipotoxicity Kidney360 2022-08-25 [PMID: 36176646] (ICC/IF, Human)
• Shibuya K, Ebihara K, Ebihara C et al. AAA-ATPase valosin-containing protein (VCP) binds the transcription factor SREBP1 and promotes its proteolytic activation by rhomboid protease RHBDL4 The Journal of biological chemistry 2022-04-14 [PMID: 35430252] (WB, Mouse)
• Samadi O Characterization of White Adipose Tissue Depots After Burn Thesis (WB, Mouse)
• Pritchard KA, Jing X, Teng M et al. Role of endoplasmic reticulum stress in impaired neonatal lung growth and bronchopulmonary dysplasia PloS one 2022-08-26 [PMID: 36018859] (IHC-P, WB, Rat)
• Abbonante V, Malara A, Chrisam M et al. Lack of COL6/collagen VI causes megakaryocyte dysfunction by impairing autophagy and inducing apoptosis Autophagy 2022-07-20 [PMID: 35857791] (WB, Human, Mouse)
• Hu JM, Chang YL, Hsieh CC, Huang SM The Synergistic Cytotoxic Effects of GW5074 and Sorafenib by Impacting Mitochondrial Functions in Human Colorectal Cancer Cell Lines Frontiers in oncology 2022-06-07 [PMID: 35747833] (WB)
• Wadgaonkar P, Bi Z, Wan J et al. Arsenic Activates the ER Stress-Associated Unfolded Protein Response via the Activating Transcription Factor 6 in Human Bronchial Epithelial Cells Biomedicines 2022-04-22 [PMID: 35625704] (IHC-P, Human)
• Lin J, Lin Y, Liu J et al. Norketamine, the Main Metabolite of Ketamine, Induces Mitochondria-Dependent and ER Stress-Triggered Apoptotic Death in Urothelial Cells via a Ca2+-Regulated ERK1/2-Activating Pathway International Journal of Molecular Sciences 2022-04-23 [PMID: 35563057] (WB, Human)
• White A, Parekh RU, Theobald D et al. Kinin B1R Activation Induces Endoplasmic Reticulum Stress in Primary Hypothalamic Neurons Frontiers in pharmacology 2022-03-08 [PMID: 35350763] (WB, Mouse)
• Kyeong M, Lee JS Endogenous BiP reporter system for simultaneous identification of ER stress and antibody production in Chinese hamster ovary cells Metabolic engineering 2022-02-17 [PMID: 35182754]
• Khoi C, Xiao C, Hung K et al. Oxidative Stress-Induced Growth Inhibitor (OSGIN1), a Target of X-Box-Binding Protein 1, Protects Palmitic Acid-Induced Vascular Lipotoxicity through Maintaining Autophagy Biomedicines [PMID: 35625730] (WB, Human)
• Lu J, Xiong K, Qian X et al. Spata7 is required for maintenance of the retinal connecting cilium Scientific reports 2022-04-02 [PMID: 35368022] (IF/IHC, Mouse)
• Rudalska R, Harbig J, Snaebjornsson M et al. LXR alpha activation and Raf inhibition trigger lethal lipotoxicity in liver cancer Nature Cancer 2021-02-01 [PMID: 35122079]
• Pan JX, Sun D, Lee D Et al. Osteoblastic Swedish mutant APP expedites brain deficits by inducing endoplasmic reticulum stress-driven senescence Communications biology 2021-11-25 [PMID: 34824365]
• Yuchang Li, Liting Chen, Lu Li, Chantal Sottas, Stephanie K. Petrillo, Anthoula Lazaris, Peter Metrakos, Hangyu Wu, Yuji Ishida, Takeshi Saito, Lucy Golden-Mason, Hugo R. Rosen, Jeremy J. Wolff, Cristina I. Silvescu, Samuel Garza, Garett Cheung, Tiffany Huang, Jinjiang Fan, Martine Culty, Bangyan Stiles, Kinji Asahina, Vassilios Papadopoulos Cholesterol-binding translocator protein TSPO regulates steatosis and bile acid synthesis in nonalcoholic fatty liver disease iScience 2021-05-01 [PMID: 34013171]
• Kornicka-Garbowska K, Bourebaba L, ROcken M, Marycz K Sex Hormone Binding Globulin (SHBG) Mitigates ER Stress in Hepatocytes In Vitro and Ex Vivo Cells 2021-03-30 [PMID: 33808055] (WB, Human)
• Koepke LG, Schwedhelm E, Ibing W et al. Spinal Stroke: Outcome Attenuation by Erythropoietin and Carbamylated Erythropoietin and Its Prediction by Sphingosine-1-Phosphate Serum Levels in Mice International journal of molecular sciences 2022-08-23 [PMID: 36076955] (IHC-P, Mouse)
• Kim HM, Choi HY, Cho GH Et al. Natural Thiols, but Not Thioethers, Attenuate Patulin-Induced Endoplasmic Reticulum Stress in HepG2 Cells Toxins 2021-10-14 [PMID: 34679020] (WB)
• Chen R, Michaeloudes C, Liang Y Et al. ORMDL3 regulates cigarette smoke-induced endoplasmic reticulum stress in airway smooth muscle cells The Journal of allergy and clinical immunology 2021-10-05 [PMID: 34624393] (WB, Human)
• Guo HH, Xiong L, Pan JX et al. Hepcidin contributes to Swedish mutant APP-induced osteoclastogenesis and trabecular bone loss Bone research 2021-06-09 [PMID: 34108442] (Mouse)
• Pandey R, Bakay M, Strenkowski BP et al. JAK/STAT inhibitor therapy partially rescues the lipodystrophic autoimmune phenotype in Clec16a KO mice Scientific reports 2021-04-01 [PMID: 33795715] (WB, Mouse)
• Chen X, Li C, Liu J et al. Inhibition of ER stress by targeting the IRE1alpha-TXNDC5 pathway alleviates crystalline silica-induced pulmonary fibrosis International immunopharmacology 2021-03-07 [PMID: 33691254] (WB, Mouse)
• Cao X, An J, Cao Y et al. EMC3 Is Essential for Retinal Organization and Neurogenesis During Mouse Retinal Development Investigative ophthalmology & visual science 2021-02-01 [PMID: 33605987] (ICC/IF, WB, Mouse)
• Elahe Zarini-Gakiye, Gholamhassan Vaezi, Kazem Parivar, Nima Sanadgol Age and Dose-Dependent Effects of Alpha-Lipoic Acid on Human Microtubule- Associated Protein Tau-Induced Endoplasmic Reticulum Unfolded Protein Response: Implications for Alzheimer's Disease. CNS & neurological disorders drug targets 2022-01-12 [PMID: 33573583]
• Kalish BT, Kim E, Finander B et al. Maternal immune activation in mice disrupts proteostasis in the fetal brain Nature neuroscience 2020-12-23 [PMID: 33361822]
• Novais EJ, Choi H, Madhu V et al. Hypoxia and Hypoxia-Inducible Factor-1 alpha Regulate Endoplasmic Reticulum Stress in Nucleus Pulposus Cells: Implications of Endoplasmic Reticulum Stress for Extracellular Matrix Secretion The American journal of pathology 2020-12-08 [PMID: 33307037]
• Menzie-Suderam JM, Modi J, Xu H et al. Granulocyte-colony stimulating factor gene therapy as a novel therapeutics for stroke in a mouse model J Biomed Sci 2020-12-01 [PMID: 33126859] (WB, Mouse)
• M Navas-Madr, E Castelblan, M Camacho, M Consegal, A Ramirez-Mo, MR Sarrias, P Perez, N Alonso, M Galán, D Mauricio Role of the Scavenger Receptor CD36 in Accelerated Diabetic Atherosclerosis Int J Mol Sci, 2020-10-05;21(19):. 2020-10-05 [PMID: 33028031] (Western Blot)
• Kim S, Lee S, Lee H et al. A Colon-Targeted Prodrug, 4-Phenylbutyric Acid-Glutamic Acid Conjugate, Ameliorates 2,4-Dinitrobenzenesulfonic Acid-Induced Colitis in Rats Preprints 2020-07-20 [PMID: 32899177] (WB, Human)
• Aryal YP, Lee ES, Kim TY et al. Stage-specific expression patterns of ER stress-related molecules in mice molars: Implications for tooth development Gene Expr. Patterns 2020-08-03 [PMID: 32758541] (IHC-P, Mouse)
• Ganesan M, Mathews S, Makarov E et al. Acetaldehyde Suppresses HBV-MHC Class I Complex Presentation on Hepatocytes Via Induction of ER Stress and Golgi Fragmentation Am. J. Physiol. Gastrointest. Liver Physiol. 2020-08-05 [PMID: 32755306]
• Cai EP, Ishikawa Y, Zhang W et al. Genome-scale in vivo CRISPR screen identifies RNLS as a target for beta cell protection in type 1 diabetes Nat Metab 2020-07-27 [PMID: 32719542]
• Hotokezaka Y, Katayama I, Nakamura T ATM-associated signalling triggers the unfolded protein response and cell death in response to stress Commun Biol 2020-07-14 [PMID: 32665601] (WB, Human)
• Benade J, Sher L, De Klerk S et al. The Impact of Sugar-Sweetened Beverage Consumption on the Liver: A Proteomics-based Analysis Antioxidants (Basel) 2020-07-01 [PMID: 32630236] (WB, Rat)
• Debernardi J, Pioche-Durieu C, Cam EL et al. Verotoxin-1-Induced ER Stress Triggers Apoptotic or Survival Pathways in Burkitt Lymphoma Cells Toxins (Basel) 2020-05-11 [PMID: 32403276] (WB, Human)
• Peserico D, Stranieri C, Garbin U et al. Ezetimibe Prevents Ischemia/Reperfusion-Induced Oxidative Stress and Up-Regulates Nrf2/ARE and UPR Signaling Pathways Antioxidants (Basel) 2020-04-23 [PMID: 32340270] (WB, Human)
• Liu D, Zhang P, Zhou J et al. TNFAIP3 Interacting Protein 3 Overexpression Suppresses Nonalcoholic Steatohepatitis by Blocking TAK1 Activation Cell Metab. 2020-04-07 [PMID: 32268115]
• Iyer S, Melendez-Suchi C et al. Elevation of the unfolded protein response increases RANKL expression. FASEB Bioadv 2020-01-04 [PMID: 32259048] (WB, Mouse)
• Lahteenvuo J, Hatinen OP, Kuivanen A et al. Susceptibility to Cardiac Arrhythmias and Sympathetic Nerve Growth in VEGF-B Overexpressing Myocardium Mol. Ther. 2020-03-19 [PMID: 32243833]
• Mahameed M,Boukeileh S,Obiedat A et Al. Pharmacological induction of selective endoplasmic reticulum retention as a strategy for cancer therapy Nat Commun 2020-03-11 [PMID: 32161259] (WB, Human)
• Upadhyay A, Sinha Ra, Kumar A, Godbole Mm Time-restricted feeding ameliorates maternal high-fat diet-induced fetal lung injury Exp. Mol. Pathol. 2020-03-06 [PMID: 32151561] (WB, Rat)
• Yap J, Chen X, Delmotte PF, Sieck GC TNF alpha Selectively Activates the IRE1 alpha/XBP1 Endoplasmic Reticulum Stress Pathway in Human Airway Smooth Muscle Cells Am. J. Physiol. Lung Cell Mol. Physiol. 2020-01-15 [PMID: 31940218]
• Modi J, Menzie-Suderam J, Xu H et al. Mode of action of granulocyte-colony stimulating factor (G-CSF) as a novel therapy for stroke in a mouse model J. Biomed. Sci. 2020-01-06 [PMID: 31907023] (WB, Mouse)
• Yang B, Lu X, Li Y et al. A Proteomic Network Approach across the Kidney Stone Disease Reveals Endoplasmic Reticulum Stress and Crystal-Cell Interaction in the Kidney Oxidative Medicine and Cellular Longevity 2019-10-27 [PMID: 31772715] (WB, Human, Rat)
• Gu R, Huang T, Xiao J et al. The IRE1 alpha Arm of UPR Regulates Muscle Cells Immune Characters by Restraining p38 MAPK Activation Front Physiol 2019-09-19 [PMID: 31607947] (WB, IF/IHC, Mouse)
• Sun, X;Zhang, X;Zhai, H;Zhang, D;Ma, S; Chicoric acid (CA) induces autophagy in gastric cancer through promoting endoplasmic reticulum (ER) stress regulated by AMPK Biomed. Pharmacother. 2019-10-01 [PMID: 31545234] (WB, Mouse)
• Prieto K, Cao Y, Mohamed E et al. Polyphenol-rich extract induces apoptosis with immunogenic markers in melanoma cells through the ER stress-associated kinase PERK Cell Death Discov 2019-09-09 [PMID: 31531232] (KO, IB, Plant, Mouse)
  
  Details:
  The role of ER stress in the mediation of Caesalpinia Spinosa-induced apoptosis and immunogenic cancer cell death in mouse melanoma cells is explored.
• Chung YP, Yen CC, Tang FC et al. Methylmercury exposure induces ROS/Akt inactivation-triggered endoplasmic reticulum stress-regulated neuronal cell apoptosis Toxicology 2019-07-19 [PMID: 31330229] (WB, Mouse)
• Kirchner P, Bourdenx M, Madrigal-Matute J et al. Proteome-wide analysis of chaperone-mediated autophagy targeting motifs PLoS Biol. 2019-05-01 [PMID: 31150375] (WB, Mouse)
• Hernandez-Alvarez MI, Sebastian D, Vives S et al. Deficient Endoplasmic Reticulum-Mitochondrial Phosphatidylserine Transfer Causes Liver Disease Cell 2019-05-02 [PMID: 31051106]
• Groenendyk, J;Robinson, A;Wang, Q;Hu, M;Tang, J;Chen, XZ;Mengel, M;Alexander, RT;Agellon, LB;Michalak, M; Tauroursodeoxycholic acid attenuates cyclosporine-induced renal fibrogenesis in the mouse model Biochim Biophys Acta Gen Subj 2019-04-24 [PMID: 31028822] (WB, Mouse)
• Wang, F;Pulinilkunnil, T;Flibotte, S;Nislow, C;Vlodavsky, I;Hussein, B;Rodrigues, B; Heparanase protects the heart against chemical or ischemia/reperfusion injury J. Mol. Cell. Cardiol. 2019-04-17 [PMID: 31004678] (WB, Mouse)
• Guo, Q;Hu, H;Liu, X;Yang, D;Yin, Y;Zhang, B;He, H;Oh, Y;Wu, Q;Liu, C;Gu, N; C/EBP beta mediates palmitate-induced musclin expression via the regulation of PERK/ATF4 pathways in myotubes Am. J. Physiol. Endocrinol. Metab. 2019-04-09 [PMID: 30964708] (WB, Mouse)
• Przystal JM, Waramit S, Pranjol MZI et al. Efficacy of systemic temozolomide-activated phage-targeted gene therapy in human glioblastoma EMBO Mol Med 2019-02-26 [PMID: 30808679] (WB, Human)
• Kim JL, Lee DH, Jeong S et al. Imatinib-induced apoptosis of gastric cancer cells is mediated by endoplasmic reticulum stress. Oncol. Rep. 2018-12-19 [PMID: 30569109] (Human)
• Huang Q, Lan T, Lu J et al. DiDang Tang Inhibits Endoplasmic Reticulum Stress-Mediated Apoptosis Induced by Oxygen Glucose Deprivation and Intracerebral Hemorrhage Through Blockade of the GRP78-IRE1/PERK Pathways Front Pharmacol 2018-12-04 [PMID: 30564125] (WB, Rat)
• Thams S, Lowry E, Larraufie M et al. A stem cell-based screening platform identifies compounds that desensitize motor neurons to endoplasmic reticulum stress Am. J. Chin. Med. 2018-10-04 [PMID: 30446391] (WB)
• Ayaub EA, Tandon k, Padwal M et al. IL-6 mediates ER expansion during hyperpolarization of alternatively activated macrophages. Immunol. Cell Biol. 2018-10-09 [PMID: 30298952] (ICC/IF, Mouse)
• Fink EE, Moparthy S, Bagati A et al. XBP1-kLF9 Axis Acts as a Molecular Rheostat to Control the Transition from Adaptive to Cytotoxic Unfolded Protein Response. Cell Rep. 2018-10-02 [PMID: 30282030] (WB, Mouse)
• Pang X, Zhang Y, Shi X et al. ERp44 depletion exacerbates ER stress and aggravates diabetic nephropathy in db/db mice. Biochem. Biophys. Res. Commun. 2018-09-14 [PMID: 30224065] (WB, Mouse)
• Burman A, Kropski JA, Calvi CL et al. Localized hypoxia links ER stress to lung fibrosis through induction of C/EBP homologous protein JCI Insight 2018-08-23 [PMID: 30135303] (WB, Mouse)
• Ogata S, Kameda K, Kono T et al. Expressions of ATF6, XBP1, and GRP78 in normal tissue, atypical adenomatous hyperplasia, and adenocarcinoma of the lung Hum. Pathol. 2018-08-16 [PMID: 30121368] (WB, Human)
• Kristensen CM, Dethlefsen MM, Tondering AS et al. PGC-1a in hepatic UPR during high-fat high-fructose diet and exercise training in mice Physiol Rep 2018-08-01 [PMID: 30105901] (WB, Mouse)
• Lewis SA, Takimoto T, Mehrvar S et al. The effect of Tmem135 overexpression on the mouse heart PLoS ONE 2018-08-13 [PMID: 30102730] (WB, Mouse)
• kristensen CM, Olsen MA, Jessen H et al. PGC-1a in exercise and fasting-induced regulation of hepatic UPR in mice. Pflugers Arch. 2018-06-30 [PMID: 29961149] (WB, Mouse)
• kristensen CM, Jessen H, Ringholm S, Pilegaard H. Muscle PGC-1a in exercise and fasting-induced regulation of hepatic UPR in mice. Acta Physiol (Oxf). 2018-06-25 [PMID: 29939478] (WB, Mouse)
• Deodati A, Argemi J, Germani D et al. The exposure to uteroplacental insufficiency is associated with activation of unfolded protein response in postnatal life. PLoS ONE. 2018-06-13 [PMID: 29897997] (WB, Rat)
• Starr CR, Pitale PM, Gorbatyuk M. Translational attenuation and retinal degeneration in mice with an active integrated stress response Cell Death Dis 2018-04-30 [PMID: 29706649] (WB, Mouse)
• Lebeau P, Byun JH, Yousof T, Austin RC. Pharmacologic inhibition of S1P attenuates ATF6 expression, causes ER stress and contributes to apoptotic cell death Toxicol. Appl. Pharmacol. 2018-04-22 [PMID: 29689241] (IB, Human)
• Rubio-Patino C, Bossowski JP, De Donatis GM. Low-Protein Diet Induces IRE1A-Dependent Anticancer Immunosurveillance. Cell metabolism. 1905-07-10 [PMID: 29551590] (WB)
• Guo J, Cui Y, Liu Q et al. Piperine ameliorates SCA17 neuropathology by reducing ER stress. Mol Neurodegener 2018-01-30 [PMID: 29378605] (Mouse)
• Xue A, Lin J, Que C, Yu Y. Aberrant endoplasmic reticulum stress mediates coronary artery spasm through regulating MLCK/MLC2 pathway. Exp. Cell Res. 2018-01-31 [PMID: 29378169] (Mouse)
• Park HJ, Park JY, Kim JW et al. Regulation of the Endoplasmic Reticulum Stress by BIP/GRP78 is involved in Meiotic Maturation of Porcine Oocytes In Vitro Dev Reprod 2017-12-01 [PMID: 29354786] (WB, Porcine)
• Amer SM, Bhopale KK, Kakumanu RD et al. Hepatic alcohol dehydrogenase deficiency induces pancreatic injury in chronic ethanol feeding model of deer mice. Exp Mol Pathol 2018-02-01 [PMID: 29337245]
• Amit U Joshi, Nay L Saw, Hannes Vogel, Anna D Cunnigham, Mehrdad Shamloo, Daria Mochly‐Rosen Inhibition of Drp1/Fis1 interaction slows progression of amyotrophic lateral sclerosis EMBO Molecular Medicine 2018-01-15 [PMID: 29335339]
• Hyer ML, Milhollen MA, Ciavarri J et al. A small-molecule inhibitor of the ubiquitin activating enzyme for cancer treatment Nat. Med. 2018-01-15 [PMID: 29334375] (WB, Human)
• Huang YL, Shen ZQ, Wu CY et al. Comparative proteomic profiling reveals a role for Cisd2 in skeletal muscle aging Aging Cell. 2017-11-23 [PMID: 29168286] (WB, Mouse)
• Hao L, Sun Q, Zhong W et al. Mitochondria-targeted ubiquinone (MitoQ) enhances acetaldehyde clearance by reversing alcohol-induced posttranslational modification of aldehyde dehydrogenase 2: A molecular mechanism of protection against alcoholic liver disease Redox Biol. [PMID: 29156373] (WB, Mouse)
• Park HJ, Park JY, Kim JW et al. Melatonin improves the meiotic maturation of porcine oocytes by reducing endoplasmic reticulum stress during in vitro maturation J. Pineal Res. 2017-11-17 [PMID: 29149522] (Porcine)
• Tang X, Snowball JM, Xu Y et al. EMC3 coordinates surfactant protein and lipid homeostasis required for respiration J. Clin. Invest. 2017-10-30 [PMID: 29083321] (IHC-P, Mouse)
• Doran AC, Ozcan L, Cai B et al. CAMKIIg suppresses an efferocytosis pathway in macrophages and promotes atherosclerotic plaque necrosis. J. Clin. Invest. 2017-11-01 [PMID: 28972541] (WB, Chemotaxis, Mouse)
• Lee S, Kim S, Hwang S et al. Dysregulated expression of proteins associated with ER stress, autophagy and apoptosis in tissues from nonalcoholic fatty liver disease Oncotarget. 2017-09-07 [PMID: 28968997] (WB, Human)
• Ahlstrom P, Rai E, Chakma S et al. Adiponectin improves insulin sensitivity via activation of autophagic flux. J. Mol. Endocrinol. 2017-11-01 [PMID: 28954814] (WB)
• Kristensen CM, Brandt CT, Ringholm S, Pilegaard H. PGC-1alpha in aging and lifelong exercise training-mediated regulation of UPR in mouse liver Exp. Gerontol. 2017-08-08 [PMID: 28801170] (Mouse)
• Carraro RS, Souza GF, Solon C et al. Hypothalamic mitochondrial abnormalities occur downstream of inflammation in diet-induced obesity Mol. Cell. Endocrinol. 2017-07-29 [PMID: 28760600] (WB, Mouse)
• Wang WA, Liu WX, Durnaoglu S et al. Loss of Calreticulin Uncovers a Critical Role for Calcium in Regulating Cellular Lipid Homeostasis Sci Rep 2017-07-19 [PMID: 28725049] (WB, Mouse)
• Pinkaew D, Chattopadhyay A, King MD et al. Fortilin binds IRE1alpha and prevents ER stress from signaling apoptotic cell death. Nat Commun. 2017-05-26 [PMID: 28550308] (IB, Human)
• Kwon K, Kwon YS, Kim SW et al. Luteolin-induced apoptosis through activation of endoplasmic reticulum stress sensors in pheochromocytoma cells. Mol Med Rep 2017-05-12 [PMID: 28498401] (WB, Rat)
• Freis P, Bollard J, Lebeau J et al. mTOR inhibitors activate PERK signaling and favor viability of gastrointestinal neuroendocrine cell lines. Oncotarget. [PMID: 28423496] (ICC/IF, Mouse)
• Kemter E, Frohlich T, Arnold GJ et al. Mitochondrial Dysregulation Secondary to Endoplasmic Reticulum Stress in Autosomal Dominant Tubulointerstitial Kidney Disease -UMOD (ADTKD-UMOD) Sci Rep. [PMID: 28220896] (WB, Mouse)
• Teng RJ, Jing X, Michalkiewicz T et al. Attenuation of endoplasmic reticulum stress by caffeine ameliorates hyperoxia-induced lung injury. Am. J. Physiol. Lung Cell Mol. Physiol. 2017-05-01 [PMID: 28213471] (Rat)
• Pinto , Paula A, Rocha d et al. Excessive training is associated with endoplasmic reticulum stress, but not apoptosis in the hypothalamus of mice. Applied Physiology, Nutrition, and Metabolism. 2016-12-05 [PMID: 28177743]
• Liu J, Wang Y, Song L et al. A critical role of DDRGK1 in endoplasmic reticulum homoeostasis via regulation of IRE1a stability Nat Commun 2017-01-27 [PMID: 28128204] (WB, Human)
• Jin J K, Blackwood E A et al. ATF6 Decreases Myocardial Ischemia/Reperfusion Damage and Links ER Stress and Oxidative Stress Signaling Pathways in the Heart. Circ Res 2017-03-03 [PMID: 27932512] (WB, Mouse)
• Tripathi M, Zhang CW, Singh BK et al. Hyperhomocysteinemia causes ER stress and impaired autophagy that is reversed by Vitamin B supplementation. Cell Death Dis. 2016-12-08 [PMID: 27929536] (WB, Mouse)
• Teng YC, Neo JC, Wu JC et al. Expression of a hepatitis B virus pre-S2 deletion mutant in the liver results in hepatomegaly and hepatocellular carcinoma in mice. J. Pathol. 2016-11-21 [PMID: 27868197] (WB, Mouse)
• Homma T, Fujii J. Heat stress promotes the down-regulation of IRE1alpha in cells: An atypical modulation of the UPR pathway. Exp. Cell Res. 2016-10-12 [PMID: 27743894] (WB, Human)
• Fan Y, He JJ, HIV-1 Tat induces unfolded protein response and endoplasmic reticulum stress in astrocytes and causes neurotoxicity through GFAP activation and aggregation J. Biol. Chem. 2016-09-08 [PMID: 27609520] (WB, Human)
• Kusama H, Kon K, Ikejima K et al. Sodium 4-phenylbutyric acid prevents murine acetaminophen hepatotoxicity by minimizing endoplasmic reticulum stress. J. Gastroenterol. 2016-09-06 [PMID: 27599972] (WB)
• Kobylewski SE, Henderson KA, Yamada KE, Eckhert CD. Activation of the EIF2alpha/ATF4 and ATF6 Pathways in DU-145 Cells by Boric Acid at the Concentration Reported in Men at the US Mean Boron Intake Biol Trace Elem Res 2016-09-01 [PMID: 27587023] (WB, Human)
• Krawczyk Kk, Ekman M, Rippe C et al. Assessing the contribution of thrombospondin-4 induction and ATF6a activation to endoplasmic reticulum expansion and phenotypic modulation in bladder outlet obstruction. Sci Rep 2016-09-01 [PMID: 27581066] (WB)
• Zheng X, Andruska N, Lambrecht MJ et al. Targeting multidrug-resistant ovarian cancer through estrogen receptor alpha dependent ATP depletion caused by hyperactivation of the unfolded protein response. Oncotarget 2016-07-24 [PMID: 27463013]
• Barrera MJ, Aguilera S, Castro I et al. Pro-inflammatory cytokines enhance ERAD and ATF6 alpha pathway activity in salivary glands of Sjogren's syndrome patients J. Autoimmun. 2016-07-23 [PMID: 27461470] (ICC/IF)
• Pailler E, Oulhen M, Billiot F et al. Method for semi-automated microscopy of filtration-enriched circulating tumor cells BMC Cancer 2016-07-15 [PMID: 27417942] (WB, Mouse)
• Shirato T, Homma T, Lee J et al. Oxidative stress caused by a SOD1 deficiency ameliorates thioacetamide-triggered cell death via CYP2E1 inhibition but stimulates liver steatosis Arch. Toxicol. 2016-06-27 [PMID: 27349771] (WB)
• Sunami Y, Ringelhan M, Kokai E et al. Canonical NFkB signaling in hepatocytes acts as a tumor suppressor in HBV surface antigen driven HCC by controlling the UPR Hepatology. 2016-01-11 [PMID: 26892811] (WB, Mouse)
• Li Y, Zalzala M, Jadhav K et al. Carboxylesterase 2 prevents liver steatosis by modulating lipolysis, ER stress and lipogenesis and is regulated by HNF4alpha. Hepatology. 2016-01-25 [PMID: 26806650]
• Yang TY, Yen CC, Lee KI et al. Molybdenum induces pancreatic beta-cell dysfunction and apoptosis via interdependent of JNK and AMPK activation-regulated mitochondria-dependent and ER stress-triggered pathways. Toxicol. Appl. Pharmacol. 2016-01-21 [PMID: 26806093] (WB, Rat)
  
  Details:
  ATF6 antibody was used for WB analysis of lysates from Rat's ancreatic beta-cell line RIN-m5F which was exposed or not to Molybdenum /Mo ions.
• Peng Y, Kim MJ, Hullinger R et al. Improved proteostasis in the secretory pathway rescues Alzheimer's disease in the mouse. Brain. 2016-01-19 [PMID: 26787453] (WB, Mouse)
• Pereira BC, da Rocha AL, Pinto AP et al. Excessive eccentric exercise-induced overtraining model leads to endoplasmic reticulum stress in mice skeletal muscles. Life Sci. 2015-12-18 [PMID: 26707388] (WB, Mouse)
• Zhou H, Zhu J, Yue S et al. The Dichotomy of Endoplasmic Reticulum Stress Response in Liver Ischemia-Reperfusion Injury Transplantation 2016-02-01 [PMID: 26683513] (WB, Mouse)
• Yu L, Andruska N, Zheng X, Shapiro DJ. Anticipatory activation of the unfolded protein response by epidermal growth factor is required for immediate early gene expression and cell proliferation. Mol. Cell. Endocrinol. 2015-11-06 [PMID: 26551735] (WB, Human)
• Mondal TK, Emeny RT, Gao D et al. A physical/psychological and biological stress combine to enhance endoplasmic reticulum stress. Toxicol. Appl. Pharmacol. 2015-09-21 [PMID: 26391182] (WB, Mouse)
• Sharma RB, O'Donnell AC, Stamateris RE et al. Insulin demand regulates b cell number via the unfolded protein response. J. Clin. Invest. 2015-10-01 [PMID: 26389675] (WB, Mouse)
• Cai X, Chen L, McGinnis JF. Correlation of ER stress and retinal degeneration in tubby mice. Exp. Eye Res. 2015-09-08 [PMID: 26325328] (WB, Mouse)
• Li S, Guo L, Qian P et al. Lipopolysaccharide Induces Autophagic Cell Death through the PERK-Dependent Branch of the Unfolded Protein Response in Human Alveolar Epithelial A549 Cells. Cell. Physiol. Biochem. 2015-08-17 [PMID: 26279443] (WB, Human)
• Ren F, Shi H, Zhang L et al. The dysregulation of endoplasmic reticulum stress response in acute-on-chronic liver failure patients caused by acute exacerbation of chronic hepatitis B. J. Viral Hepat. 2015-07-31 [PMID: 26234401] (WB, Human)
  
  Details:
  ATF6 antibody was used for WB analysis of activating transcription factor 6 expression in lysates of livers from normal, chronic hepatitis B/CHB and acute-on-chronic liver failure/AoCLF subjects (Figure 4b). The ATF6 expression levels were slightly increased in CHB and AoCLF subjects, but the partial/cleaved ATF6 levels were significantly upregulated in the mentioned groups of patients when compared to the normal subjects.
• Hrincius ER, Liedmann S, Finkelstein D et al. Acute Lung Injury Results from Innate Sensing of Viruses by an ER Stress Pathway Cell Rep. 2015-06-16 [PMID: 26051937] (WB, Human)
• Gharibani P, Modi J, Menzie J et al. Comparison between single and combined post-treatment with S-Methyl-N, N-diethylthiocarbamate sulfoxide (DETC-MeSO) and taurine following transient focal cerebral ischemia in rat brain. Neuroscience. 2015-05-25 [PMID: 26022360] (WB, Rat)
• Yao F, Li Z, Ehara T et al. Fatty Acid-Binding Protein 4 mediates apoptosis via endoplasmic reticulum stress in mesangial cells of diabetic nephropathy. Mol. Cell. Endocrinol. 2015-05-06 [PMID: 25958041] (WB, Human)
• Hotokezaka Y, Katayama I, van Leyen K, Nakamura, T. GSK-3beta-dependent downregulation of gamma-taxilin and alphaNAC merge to regulate ER stress responses Cell Death Dis 2015-04-17 [PMID: 25880086] (WB, Human)
• Wong MK, Nicholson CJ, Holloway AC, Hardy DB. Maternal nicotine exposure leads to impaired disulfide bond formation and augmented endoplasmic reticulum stress in the rat placenta. PLoS One 2015-01-01 [PMID: 25811377] (WB, Rat)
• Madhusudhan T, Wang H, Dong W et al. Defective podocyte insulin signalling through p85-XBP1 promotes ATF6-dependent maladaptive ER-stress response in diabetic nephropathy Nat Commun 2015-03-10 [PMID: 25754093] (WB, IHC-Fr, Human)
• Kung LH, Rajpar MH, Preziosi R et al. Increased classical endoplasmic reticulum stress is sufficient to reduce chondrocyte proliferation rate in the growth plate and decrease bone growth PLoS ONE. 2015-02-19 [PMID: 25693198] (WB, Mouse)
  
  Details:
  ATF6 antibody (clone 70B1413.1; Imgenex IMG-273) used for WB analysis of lysates from chondrocytes which were extracted from rib cartilage of 5 days old +/+ and c/c mice - see full text for protocol details, data shown in Fig 2Bi
• Ren F, Zhou L, Zhang X et al. Endoplasmic Reticulum Stress-Activated Glycogen Synthase Kinase 3beta Aggravates Liver Inflammation and Hepatotoxicity in Mice with Acute Liver Failure Inflammation. 2015-01-29 [PMID: 25630719] (WB, Mouse)
  
  Details:
  ATF6 antibody used for WB on lysates of liver tissue from the mice that were injected intraperitoneally with 700 mg/kg of d-galactosamine/d-GalN and 10ug/kg of lipopolysaccharide /LPS for 2, 4, and 6 hours (treated) or with PBS only (control). Primary antibody was employed at 1:500 -1000 dilution with ON 4C incubation which followed detection using HRP conjugated secondary antibody and SuperSignal West Pico Chemiluminescent Substrates based chemiluminescence development.
• Lu Th, Su Cc, Tang Fc et al. Chloroacetic acid triggers apoptosis in neuronal cells via a reactive oxygen species-induced endoplasmic reticulum stress signaling pathway. Chem. Biol. Interact. 2014-10-29 [PMID: 25451595] (WB, Mouse)
  
  Details:
  ATF6 antibody used for WB as ER stress marker on lysates of neuro-2a cells that were treated or not with chloroacetic acid (Fig 3A). WB also performed on cells treated with n-acetylcysteine (nAC) and/or Chloroacetic acid (Fig. 6C)
• Bernard-Marissal N, Sunyach C, Marissal T et al. Calreticulin levels determine onset of early muscle denervation by fast motoneurons of ALS model mice. Neurobiol. Dis. 2014-09-30 [PMID: 25277755] (IHC-Fr, Mouse)
  
  Details:
  ATF6 antibody used for IHC-Fr on lumbar spinal cord of crt+/+, crt+/?, SOD1G93A and SOD1G93A;crt+/? at P45 (Fig 4C) - 2 hours of post-fixation in PFA 4% followed by ON incubation in 20% sucrose-PBS, 18um sections, permeablization with 0.1% Triton X-100 1-2 hours, primary used at 1:200 dilution with ON incubation.
• Andruska N, Zheng X, Yang X et al. Anticipatory estrogen activation of the unfolded protein response is linked to cell proliferation and poor survival in estrogen receptor alpha-positive breast cancer. Oncogene. 2014-09-29 [PMID: 25263449] (WB, Human)
• Dlugos CA. ATF6 and caspase 12 expression in Purkinje Neurons in acute Slices from adult, ethanol-Fed rats. Brain Res. 2014-06-26 [PMID: 24976582] (IHC-Fr, Rat)
  
  Details:
  Figs 1 5: Cerebellum. The ATF6 mAb labelled Purkinjeneuron somas and presumptive Bergman glia in ethanol fed rats.
• Xu J, Wu F, Tian D et al. Open Reading Frame 3 of Genotype 1 Hepatitis E Virus Inhibits Nuclear Factor-kappa B Signaling Induced by Tumor Necrosis Factor-alpha in Human A549 Lung Epithelial Cells. PLoS ONE. 2014-06-25 [PMID: 24959724] (WB, Human)
  
  Details:
  Fig 4B: A549 cells. 90 kDa and 50 kDa ATF6 forms were detected.
• Liu L, Chowdhury S, Fang X et al. Attenuation of unfolded protein response and apoptosis by mReg2 induced GRP78 in mouse insulinoma cells. FEBS Lett. 2014-05-29 [PMID: 24801175] (WB, Mouse)
  
  Details:
  MIN6 mouse insulinoma cells, Fig 2. Both full-length and the 50 kDa cleaved fragment of ATF6 were observed.
• Enos RT, Velazquez KT, Murphy EA. Insight into the impact of dietary saturated fat on tissue-specific cellular processes underlying obesity-related diseases. J. Nutr. Biochem. 2014-03-12 [PMID: 24742471] (WB, Mouse)
  
  Details:
  Adipose (Fig 5A), liver (Fig 5B), skeletal muscle (Fig 5C) and heart (Fig 5D) tissue. The antibody was used to the impact of ahigh-fat diet on levels of the p50 ATF6 cleaveage fragment.
• Mamrosh JL, Lee JM, Wagner M et al. Nuclear receptor LRH-1/NR5A2 is required and targetable for liver endoplasmic reticulum stress resolution. Elife 2014-04-16 [PMID: 24737860] (WB, Mouse)
  
  Details:
  Hepatocytes, Fig 1G 2A, 2E, 5I, 5J, 6A, 6E, 7D
• Osborne BY, Yao TW, Wang XM et al. A rare variant in human fibroblast activation protein associated with ER stress, loss of enzymatic function and loss of cell surface localisation. Biochim. Biophys. Acta 2014-04-06 [PMID: 24717288] (WB, Human)
  
  Details:
  Primary skin fibroblasts, Fig 7A. ATF6 was detected at ~90 kDa.
• Li M, Liu Y, Xia F et al. Progranulin is required for proper ER stress response and inhibits ER stress-mediated apoptosis through TNFR2. Cell. Signal. 2014-04-01 [PMID: 24703938] (WB, Mouse)
  
  Details:
  Primary cultures of bone marrow stromal cells, Fig 4C.
• Shao Mengle, Shan Bo, Liu Yang et al. Hepatic IRE1alpha regulates fasting-induced metabolic adaptive programs through the XBP1s-PPARalpha axis signalling. Nat Commun. 2014-03-27 [PMID: 24670948] (WB, Mouse)
• Choudhury S, Nashine S, Bhootada Y et al. Modulation of the Rate of Retinal Degeneration in T17M RHO Mice by Reprogramming the Unfolded Protein Response. Adv. Exp. Med. Biol. 2014-03-25 [PMID: 24664731] (WB, Mouse)
  
  Details:
  Mouse retinas, Fig 58.3.
• Kaphalia L, Boroumand N, Hyunsu J et al. Ethanol metabolism, oxidative stress, and endoplasmic reticulum stress responses in the lungs of hepatic alcohol dehydrogenase deficient deer mice after chronic ethanol feeding. Toxicol. Appl. Pharmacol. 2014-03-10 [PMID: 24625836] (WB, Mouse)
  
  Details:
  Mouse lungs, Fig 7B. The level of ATF6 increased in lung from Ethanol-fed mice (Fig 7B).
• Miller M, Rosenthal P, Beppu A et al. ORMDL3 Transgenic Mice Have Increased Airway Remodeling and Airway Responsiveness Characteristic of Asthma. J. Immunol. 2014-03-12 [PMID: 24623133] (ICC/IF, Mouse)
  
  Details:
  Mouse bone marrow cells,Fig 3. Thapsigargin induced ATF6 activation. Active ATF6 translocated from the ER to the nucleus.
• Mohammad-Gharibani P, Modi J, Menzie J et al. Mode of Action of S-Methyl-N, N-Diethylthiocarbamate Sulfoxide (DETC-MeSO) as a Novel Therapy for Stroke in a Rat Model. Mol. Neurobiol. 2014-02-28 [PMID: 24573692] (WB, Rat)
  
  Details:
  Rat brain, data described but not shown. Imgenex: ATF6 (1:1,500).
• Zeindl-Eberhart E, Brandl L, Liebmann S et al. Epithelial-Mesenchymal Transition Induces Endoplasmic-Reticulum-Stress Response in Human Colorectal Tumor Cells. PLoS One 2014-01-01 [PMID: 24498091] (WB, Human)
• Huang YL, Chang CL, Tang CH et al. Extrinsic sphingosine 1-phosphate activates S1P5 and induces autophagy through generating endoplasmic reticulum stress in human prostate cancer PC-3 cells. Cell Signal 2013-12-11 [PMID: 24333325] (WB, Human)
• Tay KH, Luan Q, Croft A et al. Sustained IRE1 and ATF6 signaling is important for survival of melanoma cells undergoing ER stress. Cell. Signal. 2014-02-01 [PMID: 24240056] (WB, Human)
  
  Details:
  Melanoma cell lines treated with ATF6 or control shRNA (Figs 1a, 3d), Melanoma cell lines undergoing ER stress, Fig 4b. Major ATF6 bands were seen at 100 kDa, 70 kDa, and 50 kDa (Fig 4b). The specificity of the ATF6 antibody was validated with ATF6 shRNA
• Bhuiyan MS, Pattison JS, Osinska H et al. Enhanced autophagy ameliorates cardiac proteinopathy. J Clin Invest 2013-12-02 [PMID: 24177425] (WB, Mouse)
  
  Details:
  Mouse heart tissue lysates, Fig S6B.
• Yang C, Diiorio P, Jurczyk A et al. Pathological endoplasmic reticulum stress mediated by the IRE1 pathway contributes to pre-insulitic beta cell apoptosis in a virus-induced rat model of type 1 diabetes. Diabetologia 2013-12-01 [PMID: 24121653] (WB, Rat)
• Naidoo N, Davis JG, Zhu J et al. Aging and sleep deprivation induce the unfolded protein response in the pancreas: implications for metabolism. Aging Cell. 2014-02-01 [PMID: 24102714] (WB, Mouse)
  
  Details:
  Pancreas, Fig 1b. Sleep deprivation induced ATF6 cleavage.
• Thepparit Chutima, Khakpoor Atefeh, Khongwichit Sarawut et al. Dengue 2 infection of HepG2 liver cells results in endoplasmic reticulum stress and induction of multiple pathways of cell death. BMC Res Notes. 2013-01-01 [PMID: 24034452] (ICC/IF)
• Murray LS, Lu Y, Taggart A et al. Chemical chaperone treatment reduces intracellular accumulation of mutant collagen IV and ameliorates the cellular phenotype of a COL4A2 mutation that causes haemorrhagic stroke. Hum. Mol. Genet. 2014-01-15 [PMID: 24001601] (WB, Human)
  
  Details:
  Primary dermal fibroblasts, Fig 5A, B. ATF6 (1/2000 Imgenex).
• Iwasaki Y, Suganami T, Hachiya R et al. Activating transcription factor 4 links metabolic stress to interleukin-6 expression in macrophages. Diabetes. 2014-01-01 [PMID: 23990363] (WB, Mouse)
  
  Details:
  RAW264, Fig S2c.
• Misiewicz M, Dery MA, Foveau B et al. Identification of a novel endoplasmic reticulum stress response element regulated by XBP1. J Biol Chem. 2013-07-12 [PMID: 23737521]
• Kamiya T, Hara H, Adachi T. Effect of endoplasmic reticulum (ER) stress inducer thapsigargin on the expression of extracellular-superoxide dismutase in mouse 3T3-L1 adipocytes. J Clin Biochem Nutr. 2013-03-01 [PMID: 23525536]
• Storm P, Klausen TK, Trulsson M et al. A unifying mechanism for cancer cell death through ion channel activation by HAMLET. PLoS One. 2013-01-01 [PMID: 23505537]
• Grall S, Prunier-Mirebeau D, Tamareille S et al. Endoplasmic reticulum stress pathway involvement in local and remote myocardial ischemic conditioning. Shock. 2013-05-01 [PMID: 23481493] (WB, Rat)
  
  Details:
  ATF6 (IMG-273). WB (rat heart), Figs 3B, 5B (the 50 kDa form of ATF6 is shown in the blots).
• Lind KR, Ball KK, Cruz NF, Dienel GA. The unfolded protein response to endoplasmic reticulum stress in cultured astrocytes and rat brain during experimental diabetes. Neurochem Int. [PMID: 23411409]
• Schipanski A, Lange S, Segref A et al. A novel interaction between aging and ER overload in a protein conformational dementia. Genetics. 2013-03-01 [PMID: 23335331] (WB, Mouse)
  
  Details:
  ATF6 (IMG-273). WB (mouse brain), Fig 5B (cleaved ATF6 is shown in the blots).
• Kia A, Przystal JM, Nianiaris N et al. Dual systemic tumor targeting with ligand-directed phage and Grp78 promoter induces tumor regression. Mol Cancer Ther. 2012-12-01 [PMID: 23053496]
• Mans S, Banz Y, Mueller BU et al. The angiogenesis inhibitor vasostatin is regulated by neutrophil elastase dependent cleavage of calreticulin in AML patients. Blood 2012-08-13 [PMID: 22915645]
• Marshall JL, Holmberg J, Chou E et al. Sarcospan-dependent Akt activation is required for utrophin expression and muscle regeneration. J Cell Biol. 2012-06-25 [PMID: 22734004]
• Zheng X, Zheng X, Wang X et al. Acute hypoxia induces apoptosis of pancreatic B-cell by activation of the unfolded protein response and upregulation of CHOP. Cell Death Dis. 2012-06-14 [PMID: 22695615]
• Saporta MA, Shy BR, Patzko A et al. MpzR98C arrests Schwann cell development in a mouse model of early-onset Charcot-Marie-Tooth disease type 1B. Brain. 2012-07-01 [PMID: 22689911] (WB, Mouse)
  
  Details:
  ATF6 (IMG-273 clone 70B1413.1) WB (mouse sciatic nerves, data described but no shown).
• Coope A, Milanski M, Arruda AP et al. Chaperone insufficiency links TLR4 protein signaling to endoplasmic reticulum stress. J Biol Chem. 2012-05-04 [PMID: 22427667]
• Burnett HF, Audas TE, Liang G, Lu RR. Herpes simplex virus-1 disarms the unfolded protein response in the early stages of infection. Cell Stress Chaperones. 2012-07-01 [PMID: 22270612]
• Ling SC, Lau EK, Al-Shabeeb A et al. Response of myeloma to the proteasome inhibitor bortezomib is correlated with the unfolded protein response regulator XBP-1. Haematologica. 2012-01-01 [PMID: 21993678] (WB, Human)
  
  Details:
  ATF6 (IMG-273). 1. WB: Various human myeloma cell lines (RPMI-8226, kMS-11, OPM2, U266, kMS-18, H929) treated with bortezomib, Fig 1F. 2. WB: Human myeloma cell line (kMS-11) and two bortezomib resistant sub cell lines treated with bortezomib, Fig 3D.
• Chiang Chih-Kang, Hsu Shih-Ping, Wu Cheng-Tien et al. Endoplasmic reticulum stress implicated in the development of renal fibrosis. Mol Med. 2011-01-01 [PMID: 21863214] (WB, Rat)
• Michinaga S, Hisatsune A, Isohama Y, Katsuki H. Orexin neurons in hypothalamic slice cultures are vulnerable to endoplasmic reticulum stress. Neuroscience. 2011-09-08 [PMID: 21712074] (ICC/IF, Rat)
• Biouss G, Antounians L, Aguet J et al. Unveiling fetal brain changes in congenital diaphragmatic hernia: hypoxic injury with loss of progenitor cells, neurons, and oligodendrocytes bioRxiv 2023-09-24 (IHC-Fr, Rat)
• Lian IA, LOset M, Mundal SB et al. Increased endoplasmic reticulum stress in decidual tissue from pregnancies complicated by fetal growth restriction with and without pre-eclampsia. Placenta. 2011-11-01 [PMID: 21907405] (ICC/IF, WB, Human)
  
  Details:
  ATF6 (IMG-273). 1. WB: Human decidual basalis tissue from pregnancies complicated by pre-eclampsia, Fig 1A. 2. IF/ICC: Human trophoblasts, decidual cells, and macrophages, Fig 2.
• Akay C, Lindl KA, Shyam N et al. Activation status of integrated stress response pathways in neurones and astrocytes of HIV-associated neurocognitive disorders (HAND) cortex. Neuropathol Appl Neurobiol. 2012-04-01 [PMID: 21883374] (WB)
  
  Details:
  ATF6 antibody clone 70B1413.1 (IMG-273) for WB (fresh, frozen cortical autopsy tissue from normal and diseased donors), Fig 3, Table 4.1 Note: The antibody is described as detecting ATF6 alpha.
• Veras A, Bruzasco L, Lopes A et Al. Supplementation with CO induces lipogenesis in adipose tissue, leptin and insulin resistance in healthy Swiss mice Journal of Functional Foods 2023-07-01 (Western Blot, Mouse)
• Jeyasuria P, Subedi K, Suresh A et al. Elevated levels of uterine anti-apoptotic signaling may activate NFKB and potentially confer resistance to caspase 3-mediated apoptotic cell death during pregnancy in mice. Biol Reprod. 2011-08-01 [PMID: 21566000] (WB, Mouse)
  
  Details:
  WB: ATF6 (IMG-273) & GAPDH (IMG-5019A-1). Nuclear fractions of mouse uterus, Fig 6. Note: ATF6 (IMG-273) was seen at ~36 kDa, Fig 6.
• Oslowski CM, Urano F. Measuring ER stress and the unfolded protein response using mammalian tissue culture system. Methods Enzymol 2011-01-01 [PMID: 21266244] (WB)
• Su YC, Wu JL, Hong JR. Betanodavirus up-regulates chaperone GRP78 via ER stress: roles of GRP78 in viral replication and host mitochondria-mediated cell death. Apoptosis. 2011-03-01 [PMID: 21170590] (WB)
  
  Details:
  WB: MCF7 human breast carcinoma cells and GF-1 grouper (Fish) cell line (Fig 1A, B). Full-length and cleaved forms of ATF6 were detected.
• Wang L, Popko B, Roos RP. The unfolded protein response in familial amyotrophic lateral sclerosis. Hum Mol Genet. 2011-03-01 [PMID: 21159797] (WB, Mouse)
  
  Details:
  WB (mouse lumbar spinal cord homogenates), Fig 4A. 90 kDa and 50 kDa ATF6 forms were detected.
• Chang Kung-Chao, Chen Paul C-H, Chen Ya-Ping et al. Dominant expression of survival signals of endoplasmic reticulum stress response in Hodgkin lymphoma. Cancer Sci. 2011-01-01 [PMID: 21062387] (IF/IHC)
• Jia W, Loria RM, Park MA et al. The neuro-steroid, 5-androstene 3B,17a diol; induces endoplasmic reticulum stress and autophagy through PERK/eIF2a signaling in malignant glioma cells and transformed fibroblasts. Int J Biochem Cell Biol. 2010-12-01 [PMID: 20851775] (WB)
  
  Details:
  IMGENEX product cited: ATF6 (IMG-273). WB: T98G malignant glioma cells treated with thapsigargin, Fig 1D. Note: The ATF6 (IMG-273) antibody detected ATF6 at 98 kDa in untreated T98G cells. In T98G cells treated with thapsigargin, ATF6 cleavage products at
• Lepine S, Allegood JC, Park M et al. Sphingosine-1-phosphate phosphohydrolase-1 regulates ER stress-induced autophagy. Cell Death Differ. 2011-02-01 [PMID: 20798685] (WB)
  
  Details:
  WB: MCF7 cells transfected with ATF6 siRNA. (Fig 3D). Note, the specificity of the ATF6 IMG-273 antibody was validated with ATF6 siRNA by WB (Fig 3D).
• Deldicque L, Cani PD, Philp A et al. The unfolded protein response is activated in skeletal muscle by high-fat feeding: potential role in the downregulation of protein synthesis. Am J Physiol Endocrinol Metab. 2010-11-01 [PMID: 20501874]
  
  Details:
  WB: C2C12 murine skeletal muscle myoblasts treated with or without tunicamycin (TN), thapsigargin (TG), and palmitic acid (PA), Fig 5A. Note: ATF6 (IMG-124A) was detected in tunicamycin and thapsigargin but not in palmitic acid treated C2C12 cell line, Fi
• Toko H, Takahashi H, Kayama Y et al. ATF6 is important under both pathological and physiological states in the heart. J Mol Cell Cardiol. 2010-07-01 [PMID: 20380836] (WB, Rat)
  
  Details:
  ATF6 (IMG-273). 1. WB: Rat infarcted heart, Fig 1A.2. WB: Rat sham-operated or infarcted hearts treated with either vehicle or 4-(2-aminoethyl) benzenesulfonyl fluoride (AEBSF), Fig 2A. Note: ATF6 (IMG-273) was seen at 90 kDa and 50 kDa, Fig 1A & Fig 2A.
• Lithanatudom Pathrapol, Leecharoenkiat Amporn, Wannatung Tirawat et al. A mechanism of ineffective erythropoiesis in beta-thalassemia/Hb E disease. Haematologica. 2010-05-01 [PMID: 20015891] (Human)
• Xu YY, Guan DY, Yang M et al. All-trans-retinoic acid intensifies endoplasmic reticulum stress in N-acetylglucosaminyltransferase V repressed human hepatocarcinoma cells by perturbing homocysteine metabolism. J Cell Biochem. 2010-02-15 [PMID: 19960509] (WB, Human)
  
  Details:
  Product cited: ATF6 (IMG-273). WB: Human hepatic LO2 and hepatocarcinoma 7721 transfected GnT-V cell lines treated with DMSO or all-trans-retinoic acid (ALTRA), Fig 1C. Note: The full length ATF6 (IMG-273) antibody was expressed at 90 kDa and the cleaved
• Rajpar M Helen, McDermott Ben, Kung Louise et al. Targeted induction of endoplasmic reticulum stress induces cartilage pathology. PLoS Genet. 2009-10-01 [PMID: 19834559] (Mouse)
• Roboti P, Swanton E, High S. Differences in endoplasmic-reticulum quality control determine the cellular response to disease-associated mutants of proteolipid protein. J Cell Sci. 2009-11-01 [PMID: 19825935]
  
  Details:
  Products cited (WB), Fig 5B (HeLa and HeLa overexpressing myelin proteolipid protein):1. ATF6 mAb (IMG-273).2. ATF6 Transfected and Mock Transfected Lysates (40210). The lysates were used as a positive control to validate the specificity of the antibody.
• Guan D, Xu Y, Yang M et al. N-acetyl cysteine and penicillamine induce apoptosis via the ER stress response-signaling pathway. Mol Carcinog. 2010-01-01 [PMID: 19722195] (WB)
  
  Details:
  ATF6 (IMG-273). WB: HeLa cells treated with N-acetyl cysteine (NAC) or penicillamine (PEN), Fig 3C & 3D. Note: Full length ATF6 was detected at at 90kDa and the partial or cleaved form was seen at 60 kDa.
• Bommiasamy H, Back SH, Fagone P et al. ATF6alpha induces XBP1-independent expansion of the endoplasmic reticulum. J Cell Sci. 2009-05-15 [PMID: 19420237] (WB)
  
  Details:
  WB: ATF6 transfected CHO cells (Fig 2). The antibody detected ATF6a but not ATF6b transfected cells, thereby showing that the antibody specifically recognized the ATF6a isoform. Note: The antibody was transfected ATF6a validated by WB in Fig 2.
• Kim I, Shu CW, Xu W et al. Chemical biology investigation of cell death pathways activated by endoplasmic reticulum stress reveals cytoprotective modulators of ASK1. J Biol Chem. 2009-01-16 [PMID: 19004820] (WB, Rat)
  
  Details:
  ATF6 Full-length & Active/Cleaved Forms (IMG-273). WB: Rat striatal neuroprogenitor (CSM14.1) cells treated with various compounds refer to paper for further details, Fig 3.
• Hallaert DY, Jaspers A, van Noesel CJ et al. c-Abl kinase inhibitors overcome CD40-mediated drug resistance in CLL: implications for therapeutic targeting of chemoresistant niches Blood 2008-09-18 [PMID: 18796631] (WB, Human)
  
  Details:
  Citation using the Non-Recombinant Monoclonal and Biotin format of this antibody.
• Jorgensen Ellen, Stinson Andy, Shan Lin et al. Cigarette smoke induces endoplasmic reticulum stress and the unfolded protein response in normal and malignant human lung cells. BMC Cancer. 2008-01-01 [PMID: 18694499] (WB, Human)
• Chen JC, Wu ML, Huang KC, Lin WW. HMG-CoA reductase inhibitors activate the unfolded protein response and induce cytoprotective GRP78 expression. Cardiovasc Res. 2008-10-01 [PMID: 18556704] (WB, Mouse)
  
  Details:
  WB (mouse RAW 264.7 cells), Fig. 5C.
• Zhu Y, P Fenik, G Zhan, B Sanfillipo-Cohn, N Naidoo, SC Veasey. Eif-2a protects brainstem motoneurons in a murine model of sleep apnea. J Neuroscience 28:2168-2178. [PMID: 18305250]
• Li X Endoplasmic reticulum stress sensor ATF6 as an immunometabolic modulator in hepatic tumorigenesis Thesis 2023-01-01
• Preston AJ The Cancer Protective Properties of an Elephant TP53 Retrogene Thesis 2022-01-01
• Kerbiriou M, Le Drevo MA, Ferec C, Trouve P. Coupling cystic fibrosis to endoplasmic reticulum stress: Differential role of Grp78 and ATF6. Biochim Biophys Acta. 2007-12-01 [PMID: 18022401]
• Lee SY, Lee MS, Cherla RP et al. Shiga toxin 1 induces apoptosis through the endoplasmic reticulum stress response in human monocytic cells. Cell Microbiol. 2008-03-01 [PMID: 18005243] (WB, Human)
  
  Details:
  WB (human myelogenous leukemia cell line THP-1 cells), Fig. 1A. Note: The degradation of the 90 kDa ATF6 band was detected in THP-1 cells treated with Stx1. As shown in Fig 1A the levels of immunoreactive 90 kDa ATF6 were decreased beginning 2 h after Stx
• Wei H, Kim SJ, Zhang Z et al. ER and oxidative stresses are common mediators of apoptosis in both neurodegenerative and non-neurodegenerative lysosomal storage disorders and are alleviated by chemical chaperones. Hum Mol Genet. 2008-02-15 [PMID: 17989065] (WB, Human)
  
  Details:
  WB (human LSD (lysosomal storage disease) and normal cell lines), Figs. 1A,C; 3A,B,C.
• Kim DS, Jeong SK, Kim HR et al. Effects of triglyceride on ER stress and insulin resistance. Biochem Biophys Res Commun. 2007-11-09 [PMID: 17868644] (WB)
  
  Details:
  WB (Fig 2), HepG2 cells treated with triglyceride. There was an increase of cleaved ATF6 over time with the triglyceride treatment.
• Kim KM, Pae HO, Zheng M et al. Carbon monoxide induces heme oxygenase-1 via activation of protein kinase R-like endoplasmic reticulum kinase and inhibits endothelial cell apoptosis triggered by endoplasmic reticulum stress. Circ Res. 2007-01-01 [PMID: 17823375] (WB, Human)
  
  Details:
  WB (Figs 3, 4, 6): HUVEC (human umbilical vein endothelial cells), p90ATF6 and cleaved p50ATF6 forms of ATF6 are detected.
• Roberts George G, Di Loreto Mike J, Marshall Monique et al. Hippocampal cellular stress responses after global brain ischemia and reperfusion. Antioxid Redox Signal. 2007-12-01 [PMID: 17715997] (Rat)
• Pirog-Garcia KA, Meadows RS, Knowles L et al. Reduced cell proliferation and increased apoptosis are significant pathological mechanisms in a murine model of mild pseudoachondroplasia resulting from a mutation in the C-terminal domain of COMP. Hum Mol Genet. 2007-09-01 [PMID: 17588960] (WB)
  
  Details:
  WB: Fig 9B, density measurement of Western blots of ATF6 cleavage using tissue from the xiphoid process of mice with psuedoachondroplasia. The authors report a 2.2 increase of cleaved ATF6 in mutant compared to normal chondrocytes.
• Rahmani M, Davis EM, Crabtree TR et al. The kinase inhibitor sorafenib induces cell death through a process involving induction of endoplasmic reticulum stress. Mol Cell Biol. 2007-08-01 [PMID: 17548474] (WB)
  
  Details:
  WB: Fig 3A, U937 human leukemia cells treated with sorafenib. Sorafenib is a multikinase inhibitor that induces apoptosis in human leukemia and other malignant cells. The authors show a decline in ATF6 (90 kDa band) expression in sorafenib-treated cells.
• Mao W, Fukuoka S, Iwai C et al. Cardiomyocyte apoptosis in autoimmune cardiomyopathy: mediated via endoplasmic reticulum stress and exaggerated by norepinephrine. Am J Physiol Heart Circ Physiol. 2007-09-01 [PMID: 17545481] (WB, Rabbit)
  
  Details:
  Antibodies cited: 1. ATF6 (IMG-273): WB (Fig 7), analysis of ATF6 in nuclear fraction of rabbit left ventricle myocardium tissue lysates, Authors describe cleaved forms of p90ATF6 in the nucleus: p60ATF6 and p36ATF6. 2. GAPDH (IMG-5019A): WB (Fig 4), GAPD
• Renna M, Caporaso MG, Bonatti S et al. Regulation of ERGIC-53 gene transcription in response to endoplasmic reticulum stress. J Biol Chem. 2007-08-03 [PMID: 17535801] (Chemotaxis)
  
  Details:
  ChIP: Fig 6B, Steady-state and thapsigargin (TG)-treated HeLa cells. BiP/Grp78 and ERGIC-53 promoter regions were used as PCR primers in the ChIP assays to assess DNA ATF6 protein interactions. In TG-treated cells there was endogenous ATF6 binding on both
• Lee GH, Kim HK, Chae SW et al. Bax inhibitor-1 regulates endoplasmic reticulum stress-associated reactive oxygen species and heme oxygenase-1 expression. J Biol Chem. 2007-07-27 [PMID: 17526500] (WB)
  
  Details:
  WB: Neo and BI-1 transfected human HT1080 fibrosarcoma cells treated with thapsigargin or tunicamycin [(analysis of uncleaved ATF6 (p90)], Fig. 3A-C. The results showed that the level of uncleaved ATF6 (p90) was lower in Neo than in BI-1 cells. They also
• Davenport EL, Moore HE, Dunlop AS et al. Heat shock protein inhibition is associated with activation of the unfolded protein response pathway in myeloma plasma cells. Blood. 2007-10-01 [PMID: 17525289] (WB)
  
  Details:
  WB ([analysis of cleaved ATF6 nuclear fractions of human myeloma cells (U266, H929, MMI] treated with tunicamycin, thapsigargin, HSP inhibitors, or bortezomib): Figs 1C, 2C,and 3C. Results show increase of cleaved ATF6 under certain treatment conditions.
• Ou YC, Yang CR, Cheng CL et al. Indomethacin induces apoptosis in 786-O renal cell carcinoma cells by activating mitogen-activated protein kinases and AKT. Eur J Pharmacol. 2007-06-01 [PMID: 17341418] (WB)
  
  Details:
  WB (786-O human renal carcinoma cells): Fig 4. ATF-6 and cleaved ATF-6 was detected. Cleaved ATF-6 increased following 16 h of indomethacin (50 and 200 uM) treatment, with more cleaved ATF-6 detected following 200 uM than 50 uM indomethacin treatment.
• Laybutt DR, Preston AM, Akerfeldt MC et al. Endoplasmic reticulum stress contributes to beta cell apoptosis in type 2 diabetes. Diabetologia. 2007-04-01 [PMID: 17268797]
• Nagy G, Kardon T, Wunderlich L et al. Acetaminophen induces ER dependent signaling in mouse liver. Arch Biochem Biophys. 2007-03-15 [PMID: 17207453] (WB, Mouse)
  
  Details:
  Antibodies cited: 1. ATF6 (IMG-273) [WB, Fig 5 (full-length and cleaved ATF6 in mouse liver tissue)].
• Yu CY, Hsu YW, Liao CL et al. Flavivirus infection activates the XBP1 pathway of the unfolded protein response to cope with endoplasmic reticulum stress. J Virol. 2006-12-01 [PMID: 16987981] (WB)
  
  Details:
  Antibodies cited: 1. ATF6 (IMG-273) [WB, Fig.1 (N18 cells)].
• Williams BL, Lipkin WI. Endoplasmic reticulum stress and neurodegeneration in rats neonatally infected with borna disease virus. J Virol. 2006-09-01 [PMID: 16912310] (WB, Rat)
  
  Details:
  Antibodies cited: ATF6 (IMG-273) [WB, Fig 5 (cleaved ATF6 in rat cerebellum and hippocampus tissues)].
• Renna M, Faraonio R, Bonatti S et al. Nitric oxide-induced endoplasmic reticulum stress activates the expression of cargo receptor proteins and alters the glycoprotein transport to the Golgi complex. Int J Biochem Cell Biol. 2006-01-01 [PMID: 16899390] (WB)
  
  Details:
  ATF6 (IMG-273) [WB, Fig.4 (DETA NONOate- and TG-treated J774 cells)].
• Nyborg AC, Ladd TB, Jansen K et al. Intramembrane proteolytic cleavage by human signal peptide peptidase like 3 and malaria signal peptide peptidase. FASEB J. 2006-08-01 [PMID: 16873890] (WB)
  
  Details:
  Antibodies cited: 1. ATF6 (IMG-273) [WB, Fig. 3C: Analysis of a substrate consisting of the N-terminus of ATF6 fused to a transmembrane domain suspectible to SPP cleavage in vitro]
• van Kollenburg B, van Dijk J, Garbern J et al. Glia-specific activation of all pathways of the unfolded protein response in vanishing white matter disease. J Neuropathol Exp Neurol. 2006-07-01 [PMID: 16825957] (IHC-P, Human)
  
  Details:
  Antibodies cited: 1. ATF6 (IMG-273) [IHC (paraffin), Fig.5A, 5G and 6D (human brain tissue sections)].
• Donati G, Imbriano C, Mantovani R. Dynamic recruitment of transcription factors and epigenetic changes on the ER stress response gene promoters. Nucleic Acids Res. 2006-06-06 [PMID: 16757577] (Chemotaxis)
  
  Details:
  ChIP (Fig 5), HepG2 cells treated with thapsigargin.
• DuRose JB, Tam AB, Niwa M. Intrinsic capacities of molecular sensors of the unfolded protein response to sense alternate forms of endoplasmic reticulum stress. Mol Biol Cell. 2006-07-01 [PMID: 16672378] (WB)
  
  Details:
  TF6 (IMG-273) [WB, Fig 1: full-length and cleaved ATF6 in CHO cells)], [WB, Fig 2 (full-length ATF6 in HeLa cells)].
• Kikuchi H, Almer G, Yamashita S et al. Spinal cord endoplasmic reticulum stress associated with a microsomal accumulation of mutant superoxide dismutase-1 in an ALS model. Proc Natl Acad Sci U S A. 2006-04-11 [PMID: 16595634] (WB, Mouse)
  
  Details:
  Western Blots of mouse spinal cord tissue (Fig 1A: full-length and cleaved ATF6) (Fig 6 in supplemental material: cleaved ATF6) (Fig 5C: full-length ATF6 pulled down by immunoprecipition with a Bip antibody), IF (mouse spinal cord tissues), Fig 1B.
• Bailly-Maitre B, Fondevila C, Kaldas F et al. Cytoprotective gene bi-1 is required for intrinsic protection from endoplasmic reticulum stress and ischemia-reperfusion injury. Proc Natl Acad Sci U S A. 2006-02-21 [PMID: 16478805] (WB)
  
  Details:
  ATF6 (IMG-273) [WB, Fig 3,5; Supportive Fig 7,11 (Hepatocyte cells)].
• Okumura AJ, Hatsuzawa K, Tamura T et al. Involvement of a novel Q-SNARE, D12, in quality control of the endomembrane system. J Biol Chem. 2006-02-17 [PMID: 16354670] (WB)
  
  Details:
  ATF6 (IMG-273) [WB, Fig.5 (NIH3T3 cells)].
• Abdelrahim M, Newman K, Vanderlaag K et al. 3,3'-diindolylmethane (DIM) and its derivatives induce apoptosis in pancreatic cancer cells through endoplasmic reticulum stress-dependent upregulation of DR5. Carcinogenesis. 2006-04-01 [PMID: 16332727] (WB)
  
  Details:
  ATF6 (IMG-273) 2. DR5 (IMG-120) [WB, Fig.4 and Fig. 6 (Panc-28 cells)].
• Keller SH, Platoshyn O, Yuan JX. Long QT syndrome-associated I593R mutation in HERG potassium channel activates ER stress pathways. Cell Biochem Biophys. 2005-01-01 [PMID: 16244363] (WB)
  
  Details:
  Fig 6B: WB with ATF6, showing increase of active (50 kDa ATF6) in WT/1593 and 1593/1593 HERG compared to WT/WT cells) Fig 6C: IP with ATF6 and WB with Grp78, showing increase of Grp78 in WT/1593 and 1593/1593 HERG compared to WT/WT cells; IP/WB with ATF6
• Thomas M, Yu Z, Dadgar N et al. The unfolded protein response modulates toxicity of the expanded glutamine androgen receptor. J Biol Chem. 2005-06-03 [PMID: 15799970] (ICC/IF)
  
  Details:
  Antibodies cited: ATF6 (IMG-273) [IF/ICC, Fig 2A (Quantification of nuclear active ATF6)].
• Xu W, Liu L, Charles IG, Moncada S. Nitric oxide induces coupling of mitochondrial signalling with the endoplasmic reticulum stress response. Nat Cell Biol. 2004-11-01 [PMID: 15502820] (WB)
  
  Details:
  WB (analysis of p90 and p50 ATF6): Tex293 and EcR293 cells treated with various agents, Figs 1d, 2a, and 3b. Collectively the results show an increase of p50 ATF6 under certain treatment conditions.
• Tessitore A, del P Martin M, Sano R et al. GM1-ganglioside-mediated activation of the unfolded protein response causes neuronal death in a neurodegenerative gangliosidosis. Mol Cell. 2004-09-10 [PMID: 15350219] (WB)
  
  Details:
  WB: Fig 7A, mice spinal cord tissue. Lysosomal B-galactosidase -/- mice had increased levels of the 50 kDa cleaved form of ATF6 compared to wild-type mice.
• Park HR, Tomida A, Sato S et al. Effect on tumor cells of blocking survival response to glucose deprivation. J Natl Cancer Inst. 2004-09-01 [PMID: 15339968] (WB, Human)
  
  Details:
  WB (HT1080 human fibrosarcoma cells): Fig 2a. HT1080 cells transfected with full-length ATF6(F) or active ATF6(A) and treated with 2-deoxyglucose (2DG) or tunicamycin (TM) in the presence or absence of versipelostatin (VST). ATF(F), un/under-glycosylated
• Nyborg AC, Jansen K, Ladd TB et al. A signal peptide peptidase (SPP) reporter activity assay based on the cleavage of type II membrane protein substrates provides further evidence for an inverted orientation of the SPP active site relative to presenilin. J Biol Chem. 2004-10-08 [PMID: 15252014]
  
  Details:
  Western Blot, Figs 1C, 2C, 2E, 3D: Analysis of a substrate consisting of the N-terminus of ATF6 fused to a transmembrane domain suspectible to SPP cleavage in vitro.
• Hong M, Li M, Mao C, Lee AS. Endoplasmic reticulum stress triggers an acute proteasome-dependent degradation of ATF6. J Cell Biochem. 2004-07-01 [PMID: 15211570]
• Hong M, Luo S, Baumeister P et al. Underglycosylation of ATF6 as a novel sensing mechanism for activation of the unfolded protein response. J Biol Chem. 2004-03-19 [PMID: 14699159] (WB)
  
  Details:
  Antibodies cited: 1. ATF6 (IMG-273) [WB, Fig.2B (NIH3T3 cells) and Fig2D (CH0-7 cells)].
• Jenner RG, Maillard K, Cattini N et al. Kaposi's sarcoma-associated herpesvirus-infected primary effusion lymphoma has a plasma cell gene expression profile. Proc Natl Acad Sci U S A. 2003-09-02 [PMID: 12925741] (WB)
  
  Details:
  ATF6 (IMG-273) [WB, Fig.2 (DLBCL derived DEL cells, PEL cells, and MM-derived cell lines)].
• Luo S, Lee AS. Requirement of the p38 mitogen-activated protein kinase signalling pathway for the induction of the 78 kDa glucose-regulated protein/immunoglobulin heavy-chain binding protein by azetidine stress: activating transcription factor 6 as a target for stress-induced phosphorylation. Biochem J. 2002-09-15 [PMID: 12076252] (WB)
  
  Details:
  ATF6 (IMG-273) [WB, Fig.4 (NIH3T3 cells)].

Reviews for ATF6 Antibody (NBP1-40256) (7)

Average Rating: 4.1

(Based on 7 reviews)

We have 7 reviews tested in 3 species: Human, Mouse, Rat.

reviewed by: Armen Petrosyan WB Mouse 11/19/2024

Summary

• Application: Western Blot
• Sample Tested: Liver homogenates sample
• Species: Mouse
• Lot: 70B1413

reviewed by: Armen Petrosyan IF Mouse 11/19/2024

Summary

• Application: Immunofluorescence
• Sample Tested: Primary mouse hepatocytes
• Species: Mouse
• Lot: 70B1413

reviewed by: Armen Petrosyan WB Human 06/27/2023

Summary

• Application: Western Blot
• Sample Tested: PC-3 human prostate cancer cell line
• Species: Human
• Lot: JC201-4D10

reviewed by: Verified Customer WB Human 06/27/2023

Summary

• Application: Western Blot
• Sample Tested: DU145 human prostate carcinoma cell line
• Species: Human
• Lot: JC201-4D10

reviewed by: Verified Customer IHC-P Human 01/24/2022

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Placental tissue
• Species: Human
• Lot: 70B1413.1

Comments

• Comments: Fixed paraffin-embedded sections. Antibody dilution 1:50. Incubated overnight in 4C.

reviewed by: yann Jaudouin WES protein simple Rat 09/14/2018

Summary

• Application: Other
• Sample Tested: cultured embryonic cortical neuron lysate 0
• Species: Rat
• Lot: A-7

Comments

• Comments: Lysate protein cortical neuron 0,2 mg/ml.
  test antibody: 1/25e , 1/50e , 1/100e

reviewed by: Shinobu Miyazaki WB Mouse 03/18/2016

Summary

• Application: Western Blot
• Sample Tested: Whole cell lysate
• Species: Mouse
• Lot: AB121107A-09

FAQs for ATF6 Antibody (NBP1-40256). (Showing 1 - 5 of 5 FAQs).

1. We are looking for an antibody specific to ATF6 alpha that will not cross-react with ATF6 beta. Would you please help confirm if any of the following are ATF6 alpha specific antibodies: NBP1-40256, NBP1-77251, NBP1-76675 & NBP1-41439?
   • The antibodies NBP1-77251 and NBP1-76675 are specific to ATF6 alpha and should not cross-react with ATF6 beta.
2. I learn from your company web that you are providing atf6, xbp1, cbx4 antibodies against human for immunofluorescence. And from the provided pictures, it seems very good. But I am wondering if anyone use them for immunofluorescence in human cells in your memory or in published papers? And how about them? By the way, you are providing free sample size for the atf6, xbp1 antibodies, aren't you? Could you send me some for tests? One more question, do they work on endogenous proteins but not only on overexpressed proteins?
   • These products have been validated for use in IF on human: catalog numbers NBP1-40256, NB100-80861, and NBP1-83225. Yes these antibodies should work on the native protein/endogenous protein.
3. Is there a blocking peptide available for your ATF6 antibody (NBP1-40256)?
   • This antibody was made against a partial recombinant protein (not a short peptide). As such we do not have a blocking peptide for this antibody nor do we have a blocking protein. We do however have a positive control, ready to use ATF6 lysate NBP1-70778.
4. IS THIS ATF6 ANTIBODY FOR WESTERN BLOT FROM MOUSE? SO WE NEED THE ANTI-MOUSE SECONDARY?
   • NBP1-40256 (ATF6 Antibody/70B1413.1) can be used in WB with mouse tissue/cell line samples. We have many secondary antibodies that can potentially be used with NBP1-40256. However, since the primary antibody has an isotype of IgG1, it is better to use the WB-validated anti-mouse IgG1 secondary antibodies.
5. We are having trouble with the following antibody, we did not observe any signal for ATF6 (full length or cleaved), only a non-specific band at around 37 kDa. We've tested on human MRC-5 fibrablasts. Do you have any suggestions that may be helpful to us? 
   • The nature of the protein target ATF6 is differently expressed as the unglycosylated and/or glycosylated forms, with the different levels of glycosylation (http://www.uniprot.org/uniprot/P18850#ptm_processing), in the different organs/tissues/cells under the different physiological/pathological conditions. This ATF6 Antibody/70B1413.1 is a mouse monoclonal antibody with a very narrow epitope (even though we don't map any epitopes for our antibodies, it is known that almost all monoclonal epitopes have the sizes less than 15 a.a.). In other words, NBP1-40256 can bind to the full-length ATF6 and the long fragment of the cleaved protein target (not both cleaved protein species, and see #3 below). In order to detect the full-length and both two cleaved fragments of ATF6, the immunogen has to extend before and after the cleavage site at a.a. 419/420, in both directions, and therefore the epitopes can present in both sides of the cleavage site.  ATF6 has 1 transmembrane domain, 1 leucine-zipper, and three glycosylation sites that could affect the mobility in electrophoresis. As the rule of thumb, we use 110 Da as an average of one amino acid. Therefore the full-length ATF6 gives 73,700 Da (vs. 74,585 Da calculated by the exact sequence). Since it is cleaved at a.a. 419 of ATF6, two fragments will be 419 a.a. and 251 a.a. (670 - 419). Take each of them and times 110 Da individually, and these are the results: 46,090 Da and 27,610 Da (the estimated values), respectively.  Thefore your ~37 kDa species might be the short protein species with glycosylations (please double check with #1 above). Finally, we would like to suggest two things: please include other human cell lines as the controls: http://www.proteinatlas.org/ENSG00000118217-ATF6/antibody shows the WB positive results derived from using two cell lines and one organ. These could be used as the positive control. Also, the glycosylation levels are likely to be different in the different cells/tissues/organs, and therefor the different sizes of ATF can be viewed.  The second thing is to use a real positive control lysate, such as ATF6 cell lysate or ATF6 overexpression lysate for the specificity of NBP1-40256.

Bioinformatics

• Gene Symbol: ATF6
• Entrez:
  • 22926(Human)
• Uniprot:
  • P18850()