JNK1 Antibody (228601) [Unconjugated]

Western blot shows recombinant human JNK1, JNK2, and JNK3 (1 ng/lane). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for JNK1 was detected at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Western blot shows recombinant human JNK1, JNK2, and JNK3 (1 ng/lane). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for JNK1 was detected at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Western blot shows lysates of CHP-100 human neuroblastoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for JNK1 was detected at approximately 46 kDa and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

JNK1 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: 228601
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

JNK1 Antibody (228601) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human JNK1 isoform 2
  Ser2-Ile384
  Accession # P45983-1
• Specificity: Detects human JNK1 in direct ELISAs and human, mouse, and rat JNK1 expressed predominantly as p46 JNK (isoforms 2 and/or 3, both 384 aa), with lesser amounts of p54 JNK (isoforms 1 and/or 4, both 427 aa), in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) JNK2 or rhJNK3 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAPK8
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS containing 0.02% NaN₃.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for JNK1 Antibody (228601) [Unconjugated]

• c-Jun N-terminal kinase 1
• EC 2.7.11
• EC 2.7.11.24
• JNK1 alpha protein kinase
• JNK1 beta protein kinase
• JNK1
• JNK1JNK1A2
• JNK-46
• JUN N-terminal kinase
• MAP kinase 8
• MAPK 8
• MAPK8
• mitogen-activated protein kinase 8 isoform JNK1 alpha1
• mitogen-activated protein kinase 8 isoform JNK1 beta2
• mitogen-activated protein kinase 8
• PRKM8
• PRKM8JNK
• protein kinase JNK1
• SAPK1
• SAPK1JNK21B1/2
• Stress-activated protein kinase 1
• Stress-activated protein kinase JNK1

Background

The c-Jun N-terminal kinases (JNKs) are encoded by three genes: JNK1, JNK2, and JNK3. Members of the MAPK superfamily, JNKs are activated by environmental stresses and inflammatory cytokines. JNK1, also known as SAPK1 gamma and MAPK8, is expressed as four isoforms generated by alternative splicing. JNK1 is activated by dual phosphorylation at T183 and Y185 by the MAPK kinases MKK4 and/or MKK7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for JNK1 Antibody (MAB17761)(3)

Publications using MAB17761

• C Liu, CW Zhang, Y Zhou, WQ Wong, LC Lee, WY Ong, SO Yoon, W Hong, XY Fu, TW Soong, EH Koo, LW Stanton, KL Lim, ZC Xiao, GS Dawe APP upregulation contributes to retinal ganglion cell degeneration via JNK3 Cell Death Differ., 2017-12-13;0(0):. 2017-12-13 [PMID: 29238071] (Western Blot, Mouse)
• Phosphorylation Sites Identified in the NEIL1 DNA Glycosylase Are Potential Targets for the JNK1 Kinase PLoS ONE, 2016-08-12;11(8):e0157860. 2016-08-12 [PMID: 27518429] (Western Blot, Human)
• Kato T, Noma H, Kitagawa M, Takahashi T, Oshitani N, Kitagawa S Distinct role of c-Jun N-terminal kinase isoforms in human neutrophil apoptosis regulated by tumor necrosis factor-alpha and granulocyte-macrophage colony-stimulating factor. J. Interferon Cytokine Res., 2008-04-01;28(4):235-43. 2008-04-01 [PMID: 18439101] (ELISA Development, Human)

Review for JNK1 Antibody (MAB17761) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse and Rat.

reviewed by: Verified Customer WB Mouse and Rat 12/29/2016

Summary

• Application: Western Blot
• Sample Tested: Retina
• Species: Mouse and Rat

Comments

• Comments: JNK1 antibody used at 1:2000 over night, followed by donkey anti mouse secondary antibody conjugated to peroxidase and chemiluminescence detection

Bioinformatics

• Gene Symbol: MAPK8
• Uniprot:
  • P45983-1()