NFkB p105/p50 Antibody (2J10D7) - BSA Free

Western Blot: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - Analysis of p50 in HeLa lysate in the A) absence and B) presence of immunizing peptide using p50 antibody at 5 ug/ml.

Western Blot: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - Analysis of p50 in HeLa lysate in the A) absence and B) presence of immunizing peptide using p50 antibody at 5 ug/ml.

Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - IHC-P of rabbit aorta using NB100-56583. Secondary antibody: anti-mouse histofine ( Nisherei Bioscience Inc. ref: 414131F). Development: DAB (Dako ref: K346811-2) and counterstained with Hematoxylin. Submitted via verified customer review

Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on U937 cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.

Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - analysis of human breast tumor tissue using an isotype control antibody (top) and p50 antibody (bottom) at 5 ug/ml.

Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on Jurkat cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.

Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on HeLa cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.

Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on U2-OS cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.

• Reactivity: Hu, Rt, Rb
• Applications: WB, Simple Western, Flow, IHC, KO
• Clone: 2J10D7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

NFkB p105/p50 Antibody (2J10D7) - BSA Free Summary

• Immunogen: A portion of amino acids 150-200 of human NF-kB (p50) was used as the immunogen.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NFKB1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Knockout Validated
  • Simple Western 1:10 - 1:250
  • Western Blot 2-5 ug/ml
• Control:

  HeLa Whole Cell Lysate	Loading...
  NFkB p105/p50 Overexpression Lysate	Loading...

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (86%). Rat reactivity reported in scientific literature (PMID:31850807).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for NFkB p105/p50 Antibody (2J10D7) - BSA Free

• DKFZp686C01211
• DNA binding factor KBF1
• DNA-binding factor KBF1
• EBP-1
• KBF1
• NF-kappaB
• NF-kappa-B
• NF-kappabeta
• NFKB-p105
• NFKB-p50
• nuclear factor kappa-B DNA binding subunit
• nuclear factor NF-kappa-B p105 subunit
• nuclear factor NF-kappa-B p50 subunit
• nuclear factor of kappa light polypeptide gene enhancer in B-cells 1MGC54151
• p105
• p50

Background

Principal Names: Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (NFKB1); DNA-binding factor KBF1; EBP-1; NFKB-p50; Official Gene Symbol: NFKB1 Gene ID: 4790 (Human) Gene Map Locus: 4q24 (human) NF-kB is a nuclear transcription factor activated by various extra and intracellular stimuli such as cytokines, UV radiation, stress, in injury, and by bacterial and viral products. It is involved in regulation of various cellular events including cell growth, differentiation, proliferation, apoptosis and inflammation. NFKB1 (p50), a 50KDa functional sub-unit of NF-kB, is a member of Rel protein family. It is synthesized as a p105 precursor protein and consists of an N-terminal conserved RHD-region containing nuclear localization signal, DNA-binding and dimerization domains. NFKB1 (p50) forms homodimers or heterodimerizes with p65, forming the functional NF-kB factor. NFKB1 (p50) directs the nuclear translocation of NF-kB and is instrumental in its DNA-bidning. Pathological role of NF-kB has been suggested in AIDS, hematogenic cancer cell metastasis and rheumatoid arthritis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NFkB p105/p50 Antibody (NB100-56583)(5)

Publications using NB100-56583

• Apriawan T, Widjiati W, Utomo DN et al. Periosteum-induced ossification effect in skull defect through interleukin-8 and NF-kappa B pathway: An experimental study with Oryctolagus cuniculus rabbits Surgical neurology international 2022-04-15 [PMID: 35509533] (IF/IHC, Rabbit)
• Duran Y, Karaboga I Effect of hesperetin on systemic inflammation and hepatic injury after blunt chest trauma in rats Biotech Histochem 2019-12-18 [PMID: 31850807] (IF/IHC, Rat)
• Sikorski K, Mehta A et al. A high-throughput pipeline for validation of antibodies. Nat Methods 2018-01-11 [PMID: 30377371] (Human)
  
  Details:
  Antibody validation based on denaturing gel electrophoresis of biotinylated cell lysates (PAGE) followed by mass spectrometry (MS) and antibody array analysis (MAP).
• Santhi WS, Sebastian P, Varghese BT et al. NF-kappaB and COX-2 during oral tumorigenesis and in assessment of minimal residual disease in surgical margins. Exp Mol Pathol. 2006-10-01 [PMID: 16822500]
  
  Details:
  Products used (human): 1. IkBa [IMG-127A]: IHC paraffin (normal oral mucosa, leukoplakia, and oral cancer), Fig. 1 IHC paraffin analysis (normal oral mucosa, leukoplakia, and oral cancer), Fig. 2 IHC paraffin (oral cancer: primary tumor, surgical margin,

Review for NFkB p105/p50 Antibody (NB100-56583) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Rabbit.

reviewed by: Verified Customer IHC-P Rabbit 10/02/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: aorta artery
• Species: Rabbit
• Lot: 7341-1P160201

Comments

• Comments: Antigen Retrieval: pressure cooker with solution Trilogy TM (Cell Marque ref: 920P-07). Antibody dilution: 0.07ug/mL (diluted 1/4000).
  Secondary antibody: anti-mouse histofine ( Nisherei Bioscience Inc. ref: 414131F). Development: DAB (Dako ref: K346811-2) and counterstained with Hematoxylin.

Bioinformatics

• Gene Symbol: NFKB1
• Entrez:
  • 4790(Human)
• Uniprot:
  • P25942()