ATP7b Antibody - BSA Free

Immunohistochemistry-Paraffin: ATP7b Antibody [NBP2-48523] - Staining of human Liver shows strong granular cytoplasmic positivity in hepatocytes.

Immunohistochemistry-Paraffin: ATP7b Antibody [NBP2-48523] - Staining of human Cerebellum shows moderate granular cytoplasmic positivity in Purkinje cells.

Immunohistochemistry-Paraffin: ATP7b Antibody [NBP2-48523] - Staining of human Kidney shows moderate granular cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: ATP7b Antibody [NBP2-48523] - Staining of human Testis shows moderate granular cytoplasmic positivity in Leydig cells.

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ATP7b Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: RKLQGVVRVKVSLSNQEAVITYQPYLIQPEDLRDHVNDMGFEAAIKSKVAPLSLGPIDIERLQSTNPKRPLSSANQNFNNSETLGHQGSHVVTLQLRIDGMHCKSCVLNIEENIGQLLGVQSIQVSLENKTAQVKYDPSCTSPV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATP7B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  ATP7b Recombinant Protein Antigen (NBP2-48523PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ATP7b Antibody - BSA Free

• ATPase, Cu(2+)- transporting, beta polypeptide
• ATPase, Cu++ transporting, beta polypeptide
• Copper pump 2
• copper-transporting ATPase 2
• EC 3.6.3
• EC 3.6.3.4
• PWD
• WC1
• WD
• Wilson disease-associated protein
• WNDATPase, Cu++ transporting, beta polypeptide (Wilson disease)

Background

ATP7b is an important protein for copper transport and elimination of excess copper from the body. ATP7b transports metals in and out of cells using ATP. There are 3 known isoforms of the ATP7b gene; form A is found in the liver, kidney, and brain, the shorter form B is found in brain tissue, and the third isoform, known as WND/140 KDA is found in mitochondria. Mutations in the ATP7b gene can cause Wilson's disease, an inherited disorder causing copper poisoning in the brain and liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATP7B