ATPB Antibody (JM10-90)

Western Blot: ATPB Antibody (JM10-90) [NBP2-67171] - Western blot analysis of ATPB on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: ATPB Antibody (JM10-90) [NBP2-67171] - Analysis of paraffin-embedded huaman uterus tissue using anti-ATPB antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ATPB Antibody (JM10-90) [NBP2-67171] - Analysis of paraffin-embedded mouse liver tissue using anti-ATPB antibody. Counter stained with hematoxylin.

Western Blot: ATPB Antibody (JM10-90) [NBP2-67171] - Analysis of ATPB on different cells lysates using anti-ATPB antibody at 1/500 dilution. Positive control: Line1: Hela Line 2: HepG2 Line 3: 293T

Immunocytochemistry/Immunofluorescence: ATPB Antibody (JM10-90) [NBP2-67171] - Staining ATPB in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: ATPB Antibody (JM10-90) [NBP2-67171] - Staining ATPB in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: ATPB Antibody (JM10-90) [NBP2-67171] - Staining ATPB in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ATPB Antibody (JM10-90) [NBP2-67171] - Analysis of paraffin-embedded human kidney tissue using anti-ATPB antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ATPB Antibody (JM10-90) [NBP2-67171] - Analysis of paraffin-embedded human liver tissue using anti-ATPB antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ATPB Antibody (JM10-90) [NBP2-67171] - Analysis of paraffin-embedded mouse brain tissue using anti-ATPB antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ATPB Antibody (JM10-90) [NBP2-67171] - Analysis of paraffin-embedded mouse heart tissue using anti-ATPB antibody. Counter stained with hematoxylin.

Product Discontinued

ATPB Antibody (JM10-90) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human ATPB aa 141-184 / 529. (SwissProt: P06576 Human; SwissProt: P56480 Mouse; SwissProt: P10719 Rat)
• Localization: Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ATP5F1B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ATPB Antibody (JM10-90)

• ATP synthase subunit beta, mitochondrial
• ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
• ATPMB
• ATPSBmitochondrial ATP synthase beta subunit
• EC 3.6.3
• EC 3.6.3.14
• MGC5231
• mitochondrial ATP synthetase, beta subunit

Background

ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATP5F1B