AU1 Epitope Tag Antibody - BSA Free

Western Blot: AU1 Epitope Tag Antibody [NB600-453] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/mL (1:25,000).

Western Blot: AU1 Epitope Tag Antibody [NB600-453] - analysis of overexpressed AU1-tagged recombinant proteins in S2 cell extract. Expected lanes are marked with asterisk (*). WB image submitted by a verified customer review.

• Reactivity: ET
• Applications: WB, ELISA, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

AU1 Epitope Tag Antibody - BSA Free Summary

• Immunogen: Rabbits were immunized with AU1 (DTYRYI) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000 - 1:3000
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:400
  • Immunoprecipitation 1 - 4 ug/mg lysate
  • Western Blot 1:1000 - 1:3000
• Application Notes: AU1 Epitope Tag antibody validated for WB, IF from a verified customer reviews.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AU1 Epitope Tag Antibody - BSA Free

• AU1 epitope tag
• DTYRYI epitope tag
• DTYRYI tag

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AU1 Epitope Tag Antibody (NB600-453)(15)

Publications using NB600-453

• Petersen M, Lotke R, Hopfensperger K et al. Inhibition of Infectious HIV-1 Production by Rerouting the Cellular Furin Inhibitor Serpin B8 Journal of virology 2023-06-29 [PMID: 37272794]
• Ikegame S, Carmichael JC, Wells H et al. Metagenomics-enabled reverse-genetics assembly and characterization of myotis bat morbillivirus Nature microbiology 2023-06-01 [PMID: 37142773] (WB, Epitope Tag)
  
  Details:
  Dilutions: Flow 1:1,000,WB: 1:1,000
• Lee B, Ikegame S, Carmichael J Et al. Zoonotic potential of a novel bat morbillivirus Research square 2021-09-29 [PMID: 34611656] (WB)
• Pryce R, Azarm K et al. A key region of molecular specificity orchestrates unique ephrin-B1 utilization by Cedar virus. Life Sci Alliance 2020-01-01 [PMID: 31862858] (WB)
• Hotter D, Bosso M, Jonsson KL et al. IFI16 Targets the Transcription Factor Sp1 to Suppress HIV-1 Transcription and Latency Reactivation Cell Host Microbe 2019-06-12 [PMID: 31175045]
• Zhang T, Wu C, Yang X et al. Guanylate-Binding Proteins 2 and 5 Exert Broad Antiviral Activity by Inhibiting Furin-Mediated Processing of Viral Envelope Proteins Cell Rep 2019-05-14 [PMID: 31091448] (WB, Human)
• Heusinger E, Deppe k, Sette P et al. Preadaptation of SIVsmm facilitated Env-mediated counteraction of human tetherin by HIV-2. J. Virol. 2018-07-05 [PMID: 29976668] (WB, Human)
• Hotter D, Krabbe T, Reith E et al. Primate lentiviruses use at least three alternative strategies to suppress NF-kB-mediated immune activation PLoS Pathog. 2017-08-01 [PMID: 28859166] (WB, Human)
• Mack K, Starz K, Sauter D et al. Efficient Vpu-mediated tetherin antagonism by an HIV-1 group O strain. J Virol 2017-01-11 [PMID: 28077643]
• HirmondoR, Szabo JE, Nyiri K et al. Cross-species inhibition of dUTPase via the Staphylococcal Stl protein perturbs dNTP pool and colony formation in Mycobacterium DNA Repair (Amst.) 2015-03-19 [PMID: 25841100] (WB)
• Rona G, Palinkas Hl, Borsos M et al. NLS copy-number variation governs efficiency of nuclear import - case study on dUTPases. FEBS J. 2014-10-04 [PMID: 25283549] (ICC/IF)
• Kingston D, Chang H, Ensser A et al. Inhibition of retromer activity by herpesvirus saimiri tip leads to CD4 downregulation and efficient T cell transformation. J Virol 2011-10-01 [PMID: 21849449]
• Kaletsky RL, Francica JR, Agrawal-Gamse C et al. Tetherin-mediated restriction of filovirus budding is antagonized by the Ebola glycoprotein. Proc Natl Acad Sci U S A 2009-02-01 [PMID: 19179289]
• Liu L, Li F, Cardelli JA et al. Rapamycin inhibits cell motility by suppression of mTOR-mediated S6K1 and 4E-BP1 pathways. Oncogene 2006-11-01 [PMID: 16715128]
• Gewin L, Myers H, Kiyono T et al. Identification of a novel telomerase repressor that interacts with the human papillomavirus type-16 E6/E6-AP complex. Genes Dev 2004-09-01 [PMID: 15371341]

Reviews for AU1 Epitope Tag Antibody (NB600-453) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Other.

reviewed by: Verified Customer IF Other 01/13/2015

Summary

• Application: Immunofluorescence
• Sample Tested: Expressed in S2 or WR-Dv-1 cell line
• Species: Other

reviewed by: Verified Customer WB Other 01/13/2015

Summary

• Application: Western Blot
• Sample Tested: S2 lysate (with overexpressed recombinant protein)
• Species: Other