Axin-1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Axin-1 Antibody - BSA Free [NBP1-76483] - Immunofluorescence of Axin-1 in human brain tissue with Axin-1 antibody at 20 u/mL.

Western Blot: Axin-1 Antibody [NBP1-76483] - SK-N-SH cell lysate with AXIN1 antibody at 1 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Axin-1 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human AXIN1. The immunogen is located within amino acids 620 - 670 of AXIN1. Amino Acid Squence: EKEISRHRRTGHGS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AXIN1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Western Blot 1-2 ug/ml
• Control Peptide:

  Axin-1 Peptide (NBP1-76483PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Axin-1 Antibody - BSA Free

• axin 1
• Axin1
• Axin-1
• AXINaxis inhibitor 1
• Axis inhibition protein 1
• fused, mouse, homolog of
• hAxin
• MGC52315

Background

AXIN1 is a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain and is thought to function as a negative regulator of the WNT signaling pathway that regulates embryonic axis formation. AXIN1 interacts with adenomatosis polyposis coli (APC), beta-catenin, glycogen synthase kinase 3 beta, forming a tetrameric complex resulting in the regulation of the stabilization of beta-catenin. Mutations in the AXIN1 gene have been associated various carcinomas, indicating that it also functions as a tumor suppressor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Axin-1 Antibody (NBP1-76483)(1)

Publication using NBP1-76483

• Miyatake K, Kumagai K, Imai S et al. Sclerostin inhibits interleukin-1beta-induced late stage chondrogenic differentiation through downregulation of Wnt/beta-catenin signaling pathway PLoS ONE 2020-09-25 [PMID: 32976505] (ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: AXIN1
• Uniprot:
  • AAC51624(Human)