Axl Antibody (7E10)

Western Blot: Axl Antibody (7E10) [NBP2-37675] - Analysis using AXL mouse mAb against extracellular domain of human AXL (aa19-444).

Immunocytochemistry/Immunofluorescence: Axl Antibody (7E10) [NBP2-37675] - Confocal immunofluorescence analysis of methanol-fixed HEK293 cells trasfected with AXL-hIgGFc using AXL mouse mAb(green), showing cytoplasmic and membrane localization. Blue: DRAQ5 fluorescent DNA dye.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 7E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Axl Antibody (7E10) Summary

• Immunogen: Purified recombinant extracellular fragment of human Axl fused with hIgGFc tag expressed in HEK293 cell line.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: AXL
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 97.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Axl Antibody (7E10)

• AI323647
• ARK
• AXL oncogene
• AXL receptor tyrosine kinase
• AXL transforming sequence/gene
• Axl
• EC 2.7.10
• EC 2.7.10.1
• JTK11
• Tyro7
• tyrosine-protein kinase receptor UFO
• UFO
• UFOoncogene AXL

Background

Axl is encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, this protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation and can also mediate cell aggregation by homophilic binding. Alternatively spliced transcript variants encoding different isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AXL