Recombinant Human Gas6 Protein, CF

Recombinant Human Gas6 885-GSB down regulates the expression of Axl in DU145 human prostate carcinoma cells. The ED50 for this effect is 25-150 ng/mL.

Recombinant Human Gas6 885-GSB down regulates the expression of Axl in DU145 human prostate carcinoma cells. The ED50 for this effect is 25-150 ng/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Gas6 Protein, CF Summary

• Details of Functionality: Measured by its ability to down regulate the expression of Axl in DU145 human prostate carcinoma cells. Mishra, A. et al. (2012) Mol. Cancer Res. 10:703. The ED₅₀ for this effect is 25-150 ng/mL
• Source: Mouse myeloma cell line, NS0-derived human Gas6 protein
  Ala49-Ala678, with a C-terminal 6-His tag
• Accession #: NP_000811
• N-terminal Sequence: Ala49
• Protein/Peptide Type: Recombinant Proteins
• Gene: GAS6
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 70.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 70-85 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and Citrate.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in sterile water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Gas6 Protein, CF

• AXLLG
• AXLLGAXL stimulatory factor
• AXSFAXL receptor tyrosine kinase ligand
• DKFZp666G247
• FLJ34709
• Gas6
• GAS-6
• growth arrest-specific 6
• growth arrest-specific protein 6

Background

Gas6 (Growth Arrest Specific 6) is a multimodular protein that is up-regulated by a wide variety of cell types in response to growth arrest (1). Gas6 and the structurally related Protein S are vitamin K-dependent and have an extensively gamma -carboxylated N-terminal Gla domain, four EGF-like repeats, and a C-terminal region with homology to steroid hormone binding globulin (SHBG) (2). Human Gas6 is a 75 kDa protein that shares 77%-79% aa sequence identity with mouse and rat Gas6, and 43% aa identity with human protein S (over the region expressed). Alternate splicing generates isoforms that lack the Gla domain and/or the spacer between the
EGF‑like and SHBG regions. Gas6 binds and induces signaling through the receptor tyrosine kinases Axl, Dtk, and Mer (3-5). Human Gas6 interacts with both mouse and rat orthologs of these receptors (1). The full length isoform may be cleaved, resulting in release of the free SHBG region which can independently activate Axl (6). Shed soluble forms of Axl and Mer bind Gas6 and function as decoy receptors (7, 8). Gas6 induces a variety of responses, including prevention of apoptosis (9), cell proliferation (10), platelet-mediated thrombosis (11), retinal epithelial cell phagocytosis of outer rod segments (12), inhibition of VEGF-induced endothelial cell chemotaxis (13), and the differentiation and expansion of NK cell precursors (14). The affinity of Gas6 for phosphatidylserine likely contributes to its role in promoting the phagocytosis of apoptotic cells (15). Several of these effects have been shown to require gamma -carboxylation of the Gla domain (12, 16).

1. Hafizi, S. and B. Dahlback (2006) FEBS J. 273:5231.
2. Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
3. Stitt, T.N. et al. (1995) Cell 80:661.
4. Ohashi, K. et al. (1995) J. Biol. Chem. 270:22681.
5. Nagata, K. et al. (1996) J. Biol. Chem. 271:30022.
6. Goruppi, S. et al. (1997) FEBS Lett. 415:59.
7. Sather, S. et al. (2007) Blood 109:1026.
8. Budagian, V. et al. (2005) Mol. Cell. Biol. 25:9324.
9. Shankar, S.L. et al. (2006) J. Neurosci. 26:5638.
10. Yanagita, M. et al. (2002) J. Clin. Invest. 110:239.
11. Gould, W.R. et al. (2005) J. Thromb. Haemost. 3:733.
12. Hall, M.O. et al. (2002) Exp. Eye Res. 75:391.
13. Gallicchio, M. et al. (2005) Blood 105:1970.
14. Caraux, A. et al. (2006) Nat. Immunol. 7:747.
15. Wu, Y. et al. (2005) J. Cell Sci. 118:539.
16. Hasanbasic, I. et al. (2005) J. Thromb. Haemost. 3:2790.

Show all background references

Publications for Gas6 (885-GSB)(39)

Publications using 885-GSB

• Brink, SCA;Koolen, L;Klaver, CCW;Bakker, RA;den Hollander, AI;Almedawar, S; Non-canonical roles of CFH in retinal pigment epithelial cells revealed by dysfunctional rare CFH variants Stem cell reports 2024-12-17 [PMID: 39753135] (Bioassay, Human)
• Demirsoy, S;Tran, H;Liu, J;Li, Y;Yang, S;Aregawi, D;Glantz, MJ;Jacob, NK;Walter, V;Schell, TD;Olmez, I; Targeting Tyro3, Axl, and MerTK Receptor Tyrosine Kinases Significantly Sensitizes Triple-Negative Breast Cancer to CDK4/6 Inhibition Cancers 2024-06-18 [PMID: 38927958] (Bioassay, Human)
• Lahey, KC;Varsanyi, C;Wang, Z;Aquib, A;Gadiyar, V;Rodrigues, AA;Pulica, R;Desind, S;Davra, V;Calianese, DC;Liu, D;Cho, JH;Kotenko, SV;De Lorenzo, MS;Birge, RB; Regulation of Mertk Surface Expression via ADAM17 and ?-Secretase Proteolytic Processing International journal of molecular sciences 2024-04-17 [PMID: 38673989] (Western Blot, N/A)
• Lundquist, MR;Jaffrey, SR; Gas6-Axl Signaling Induces SRF/MRTF-A Gene Transcription via MICAL2 Genes 2023-12-18 [PMID: 38137053] (Bioassay, Human)
• Nguyen, LT;Aprico, A;Nwoke, E;Walsh, AD;Blades, F;Avneri, R;Martin, E;Zalc, B;Kilpatrick, TJ;Binder, MD; Mertk-expressing microglia influence oligodendrogenesis and myelin modelling in the CNS Journal of neuroinflammation 2023-11-06 [PMID: 37926818] (Bioassay, In vivo assay, Transgenic Mouse, Xenopus)
• Serwe, G;Kachaner, D;Gagnon, J;Plutoni, C;Lajoie, D;Duramé, E;Sahmi, M;Garrido, D;Lefrançois, M;Arseneault, G;Saba-El-Leil, MK;Meloche, S;Emery, G;Therrien, M; CNK2 promotes cancer cell motility by mediating ARF6 activation downstream of AXL signalling Nature communications 2023-06-15 [PMID: 37322019] (Bioassay, Human)
• Tsugawa, H;Ohki, T;Tsubaki, S;Tanaka, R;Matsuzaki, J;Suzuki, H;Hozumi, K; Gas6 ameliorates intestinal mucosal immunosenescence to prevent the translocation of a gut pathobiont, Klebsiella pneumoniae, to the liver PLoS pathogens 2023-06-01 [PMID: 37289655] (Bioassay, Human)
• Hedrich, V;Breitenecker, K;Ortmayr, G;Pupp, F;Huber, H;Chen, D;Sahoo, S;Jolly, MK;Mikulits, W; PRAME Is a Novel Target of Tumor-Intrinsic Gas6/Axl Activation and Promotes Cancer Cell Invasion in Hepatocellular Carcinoma Cancers 2023-04-22 [PMID: 37173882] (Bioassay, Human)
• J Kim, G Nam, YK Shin, N Vilaplana-, HC Jeung, EJ Moon, IJ Lee Targeting AXL Using the AVB-500 Soluble Receptor and through Genetic Knockdown Inhibits Bile Duct Cancer Growth and Metastasis Cancers, 2023-03-21;15(6):. 2023-03-21 [PMID: 36980768] (Bioassay, Human)
• M Iida, NK McDaniel, KL Kostecki, NB Welke, CA Kranjac, P Liu, C Longhurst, JY Bruce, S Hong, R Salgia, DL Wheeler AXL regulates neuregulin1 expression leading to cetuximab resistance in head and neck cancer BMC Cancer, 2022-04-23;22(1):447. 2022-04-23 [PMID: 35461210] (Bioassay, Human)
• A Puddu, S Ravera, I Panfoli, N Bertola, D Maggi High Glucose Impairs Expression and Activation of MerTK in ARPE-19 Cells International Journal of Molecular Sciences, 2022-01-20;23(3):. 2022-01-20 [PMID: 35163068] (Bioassay, Human)
• LW Sun, SH Kao, SF Yang, SW Jhang, YC Lin, CM Chen, YH Hsieh Corosolic Acid Attenuates the Invasiveness of Glioblastoma Cells by Promoting CHIP-Mediated AXL Degradation and Inhibiting GAS6/AXL/JAK Axis Cells, 2021-10-28;10(11):. 2021-10-28 [PMID: 34831142] (Bioassay, Human)
• H Chang, R An, X Li, X Lang, J Feng, M Lv Anti-Axl monoclonal antibodies attenuate the migration of MDA-MB-231 breast cancer cells Oncology Letters, 2021-08-27;22(5):749. 2021-08-27 [PMID: 34539853] (Bioassay, ELISA (Capture), Human)
• X Wu, L Wang, NA Pearson, S Renuse, R Cheng, Y Liang, DG Mun, AK Madugundu, Y Xu, PS Gill, A Pandey Quantitative Tyrosine Phosphoproteome Profiling of AXL Receptor Tyrosine Kinase Signaling Network Cancers, 2021-08-23;13(16):. 2021-08-23 [PMID: 34439388] (Bioassay, Human)
• S Pietrobono, G Anichini, C Sala, F Manetti, LL Almada, S Pepe, RM Carr, BD Paradise, JN Sarkaria, JI Davila, L Tofani, I Battisti, G Arrigoni, L Ying, C Zhang, H Li, A Meves, ME Fernandez-, B Stecca ST3GAL1 is a target of the SOX2-GLI1 transcriptional complex and promotes melanoma metastasis through AXL Nat Commun, 2020-11-17;11(1):5865. 2020-11-17 [PMID: 33203881] (Bioassay, Human)
• W Du, J Zhu, Y Zeng, T Liu, Y Zhang, T Cai, Y Fu, W Zhang, R Zhang, Z Liu, JA Huang KPNB1-mediated nuclear translocation of PD-L1 promotes non-small cell lung cancer cell proliferation via the Gas6/MerTK signaling pathway Cell Death Differ, 2020-11-02;0(0):. 2020-11-02 [PMID: 33139930] (Bioassay, Human)
• J Xue, L Xu, H Zhu, M Bai, X Li, Z Zhao, H Zhong, G Cheng, X Li, F Hu, Y Su CD14+CD16- monocytes are the main precursors of osteoclasts in rheumatoid arthritis via expressing Tyro3TK Arthritis Research & Therapy, 2020-09-21;22(1):221. 2020-09-21 [PMID: 32958023] (Bioassay, Human)
• A Abu-Thurai, MA Goyette, J Boulais, C Delliaux, C Apcher, C Schott, R Chidiac, H Bagci, MP Thibault, D Davidson, M Ferron, A Veillette, RJ Daly, AC Gingras, JP Gratton, JF Côté AXL confers cell migration and invasion by hijacking a PEAK1-regulated focal adhesion protein network Nat Commun, 2020-07-17;11(1):3586. 2020-07-17 [PMID: 32681075] (ELISA Standard, Human)
• S Alivernini, L MacDonald, A Elmesmari, S Finlay, B Tolusso, MR Gigante, L Petricca, C Di Mario, L Bui, S Perniola, M Attar, M Gessi, AL Fedele, S Chilaka, D Somma, SN Sansom, A Filer, C McSharry, NL Millar, K Kirschner, A Nerviani, MJ Lewis, C Pitzalis, AR Clark, G Ferracciol, I Udalova, CD Buckley, E Gremese, IB McInnes, TD Otto, M Kurowska-S Distinct synovial tissue macrophage subsets regulate inflammation and remission in rheumatoid arthritis Nat. Med., 2020-06-29;0(0):. 2020-06-29 [PMID: 32601335] (Flow Cytometry, Human)
• T Hara, A Kimura, T Miyazaki, H Tanaka, M Morimoto, K Nakai, J Soeda Cabozantinib inhibits AXL- and MET-dependent cancer cell migration induced by growth-arrest-specific 6 and hepatocyte growth factor Biochem Biophys Rep, 2020-01-17;21(0):100726. 2020-01-17 [PMID: 32055714] (Cell Culture, Human)
• JP Hoj, B Mayro, AM Pendergast A TAZ-AXL-ABL2 Feed-Forward Signaling Axis Promotes Lung Adenocarcinoma Brain Metastasis Cell Rep, 2019-12-10;29(11):3421-3434.e8. 2019-12-10 [PMID: 31825826] (Cell Culture, Mouse)
• D De Silva, Z Zhang, Y Liu, JS Parker, C Xu, L Cai, GG Wang, HS Earp, YE Whang Interaction between androgen receptor and coregulator SLIRP is regulated by Ack1 tyrosine kinase and androgen Sci Rep, 2019-12-09;9(1):18637. 2019-12-09 [PMID: 31819114] (Cell Culture, Human)
• A Tutusaus, E de Gregori, B Cucarull, H Cristóbal, C Aresté, I Graupera, M Coll, A Colell, G Gausdal, JB Lorens, P García de, A Morales, M Marí A FUNCTIONAL ROLE OF GAS6/TAM IN NON-ALCOHOLIC STEATOHEPATITIS PROGRESSION IMPLICATES AXL AS THERAPEUTIC TARGET Cell Mol Gastroenterol Hepatol, 2019-11-02;0(0):. 2019-11-02 [PMID: 31689560] (Cell Culture, Human)
• T Azad, K Nouri, HJ Janse van, SM Maritan, L Wu, Y Hao, T Montminy, J Yu, P Khanal, LM Mulligan, X Yang A gain-of-functional screen identifies the Hippo pathway as a central mediator of receptor tyrosine kinases during tumorigenesis Oncogene, 2019-09-02;0(0):. 2019-09-02 [PMID: 31477837] (Bioassay, Human)
• DP Strange, B Jiyarom, N Pourhabibi, X Xie, C Baker, H Sadri-Arde, PY Shi, S Verma Axl Promotes Zika Virus Entry and Modulates the Antiviral State of Human Sertoli Cells MBio, 2019-07-16;10(4):. 2019-07-16 [PMID: 31311882] (Bioassay, Human)
• Y Duan, B Hu, C Qiao, L Luo, X Li, J Wang, H Liu, T Zhou, B Shen, M Lv, J Feng Engineered AXL(-ECD)-Fc variants that abolish the AXL/Gas6 interaction suppress tumor cell migration. Oncol Lett, 2019-04-15;17(6):5784-5792. 2019-04-15 [PMID: 31186805] (Bioassay, ELISA (Capture), Human)
• F Collina, L La Sala, F Liotti, N Prevete, E La Mantia, MG Chiofalo, G Aquino, L Arenare, M Cantile, G Liguori, F Di Gennaro, L Pezzullo, NS Losito, G Vecchio, G Botti, RM Melillo, R Franco AXL Is a Novel Predictive Factor and Therapeutic Target for Radioactive Iodine Refractory Thyroid Cancer Cancers (Basel), 2019-06-07;11(6):. 2019-06-07 [PMID: 31181609] (Bioassay, Human)
• KA Mills, JM Quinn, ST Roach, M Palisoul, M Nguyen, H Noia, L Guo, J Fazal, DG Mutch, SA Wickline, H Pan, KC Fuh p5RHH nanoparticle-mediated delivery of AXL siRNA inhibits metastasis of ovarian and uterine cancer cells in mouse xenografts Sci Rep, 2019-03-18;9(1):4762. 2019-03-18 [PMID: 30886159] (Bioassay, Human)
• L Sinik, KA Minson, JJ Tentler, J Carrico, SM Bagby, WA Robinson, R Kami, T Burstyn-Co, SG Eckhardt, X Wang, SV Frye, HS Earp, D DeRyckere, DK Graham Inhibition of MERTK Promotes Suppression of Tumor Growth in BRAF Mutant and BRAF Wild-Type Melanoma Mol. Cancer Ther., 2018-11-27;18(2):278-288. 2018-11-27 [PMID: 30482852] (Bioassay, Human)
• LC Cheng, YL Chen, AN Cheng, AY Lee, CY Cho, JS Huang, SE Chuang AXL phosphorylates and up-regulates TNS2 and its implications in IRS-1-associated metabolism in cancer cells J. Biomed. Sci., 2018-11-12;25(1):80. 2018-11-12 [PMID: 30419905] (Activation, Human)
• K Fujita, X Chen, H Homma, K Tagawa, M Amano, A Saito, S Imoto, H Akatsu, Y Hashizume, K Kaibuchi, S Miyano, H Okazawa Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathology Nat Commun, 2018-01-30;9(1):433. 2018-01-30 [PMID: 29382817] (Bioassay)
• C Dantas-Bar, T Lesluyes, FL Loarer, F Chibon, I Treilleux, JM Coindre, P Meeus, M Brahmi, O Bally, I Ray-Coquar, MP Sunyach, AL Cesne, O Mir, S Bonvalot, M Toulmonde, A Italiano, P Saintigny, M Jean-Denis, F Ducimetier, D Ranchere, H El Sayadi, L Alberti, JY Blay Expression and role of TYRO3 and AXL as potential therapeutical targets in leiomyosarcoma Br. J. Cancer, 2017-10-12;117(12):1787-1797. 2017-10-12 [PMID: 29024938] (Bioassay, Human)
• SN Cross, JA Potter, P Aldo, JY Kwon, M Pitruzzell, M Tong, S Guller, CV Rothlin, G Mor, VM Abrahams Viral Infection Sensitizes Human Fetal Membranes to Bacterial Lipopolysaccharide by MERTK Inhibition and Inflammasome Activation J. Immunol., 2017-09-15;0(0):. 2017-09-15 [PMID: 28916522] (Bioassay, Human)
• ML Palisoul, JM Quinn, E Schepers, IS Hagemann, L Guo, K Reger, AR Hagemann, CK McCourt, PH Thaker, MA Powell, DG Mutch, KC Fuh Inhibition of the Receptor Tyrosine Kinase AXL Restores Paclitaxel Chemosensitivity in Uterine Serous Cancer Mol. Cancer Ther., 2017-09-13;0(0):. 2017-09-13 [PMID: 28904132] (Bioassay, Human)
• L Xu, Y Chen, M Dutra-Clar, A Mayakonda, M Hazawa, SE Savinoff, N Doan, JW Said, WH Yong, A Watkins, H Yang, LW Ding, YY Jiang, JW Tyner, J Ching, JP Kovalik, V Madan, SL Chan, M Müschen, JJ Breunig, DC Lin, HP Koeffler BCL6 promotes glioma and serves as a therapeutic target Proc. Natl. Acad. Sci. U.S.A, 2017-03-29;0(0):. 2017-03-29 [PMID: 28356518] (Bioassay, Human)
• Kasikara C, Kumar S, Kimani S, Tsou W, Geng K, Davra V, Sriram G, Devoe C, Nguyen K, Antes A, Krantz A, Rymarczyk G, Wilczynski A, Empig C, Freimark B, Gray M, Schlunegger K, Hutchins J, Kotenko S, Birge R Phosphatidylserine Sensing by TAM Receptors Regulates AKT-Dependent Chemoresistance and PD-L1 Expression. Mol Cancer Res, 2017-02-09;15(6):753-764. 2017-02-09 [PMID: 28184013] (ELISA (Standard))
• Zhi-Min Yuan AXL receptor signalling suppresses p53 in melanoma through stabilization of the MDMX-MDM2 complex J Mol Cell Biol, 2017-04-01;0(0):. 2017-04-01 [PMID: 27927748] (Bioassay, Human)
• Wilhem Leconet Therapeutic activity of anti-AXL antibody against triple-negative breast cancer Patient Derived Xenografts and metastasis Clin. Cancer Res, 2016-12-06;0(0):. 2016-12-06 [PMID: 27923843] (Western Blot)
• KE Happonen, S Tran, M Mörgelin, R Prince, S Calzavarin, A Angelillo-, B Dahlbäck The Gas6-Axl Protein Interaction Mediates Endothelial Uptake of Platelet Microparticles J. Biol. Chem., 2016-03-22;291(20):10586-601. 2016-03-22 [PMID: 27006397] (Bioassay, Human)

Review for Gas6 (885-GSB) (1)

Average Rating: 4

(Based on 1 review)

reviewed by: Verified Customer Binding assay/Protein-protein interaction 03/21/2018

Summary

• Application: Binding assay/Protein-protein interaction
• Lot: DFGX0416121

Bioinformatics

• Gene Symbol: GAS6
• Uniprot:
  • NP_000811()