Bcl-2 Antibody - BSA Free

Western Blot: Bcl-2 Antibody [NB100-56101] - Analysis of Bcl-2 in 293T cell lysate (30ug) using anti-Bcl-2 antibody. Image from verified customer review.

Western Blot: Bcl-2 Antibody [NB100-56101] - Analysis of Bcl-2 in 293T cell lysate (30ug) using anti-Bcl-2 antibody. Image from verified customer review.

• Reactivity: Hu, Mu, Rt, Ca, Ma
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Bcl-2 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide corresponding to amino acids 67-86 (ARTSPLRPLVATAGPALSPV) of mouse and rat Bcl-2 was used as immunogen; GenBank no gb/AAP47159.1. This sequence is 100% conserved in mouse and rat Bcl-2 alpha (236 amino acids) and beta (100 amino acids) isoforms.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCL2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Bcl-2 Antibody - BSA Free

• apoptosis regulator Bcl-2
• B-cell CLL/lymphoma 2
• Bcl2
• Bcl-2

Background

The Bcl-2 family of apoptosis-related genes plays central roles in regulating apoptotic pathways (reviewed in Thomadaki and Scorilas, 2006). Regulation of cell death through apoptosis is critical for the maintenance of homeostasis, defense against infectious agents, and normal development. Bcl-2 family proteins regulate apoptosis primarily through the regulation of mitochondrial outer membrane permeability. In mammals, the family consists of both prosurvival (antiapoptotic) and proapoptotic (prodeath) members. Cellular homeostasis is thought to be dependent on a balance between the actions of prosurvival and proapoptotic proteins. Bcl-2 family proteins can be divided into 3 main subfamilies on the basis of their function and the content of their Bcl-2 homology (BH) domains, for example: 1) Prosurvival: Bcl-2, Bcl-XL, Bcl-W, A1, and Mcl-1 2) Proapoptotic (multidomain): Bax, Bak, and Bok. 3) BH3-only (proapoptotic): Bad, Bcl-XS, Bid, Bik, Bim, Blk, Bmf, Bnip, Noxa, and Puma. Prosurvival members inhibit cells from undergoing apoptosis, whereas proapoptotic and BH3-only subfamily members promote apoptosis. There are 4 BH domains (1-4) conserved among Bcl-2 family proteins. The BH domains are important for function as well as for heterodimerization between family members. Typical prosurvival family members have all four BH domains (1-4), whereas proapoptotic (multidomain) members have BH1, 2 and 3 domains and BH3-only members have only the BH3 domain. Overall, the relative ratio of prosurvival and proapoptotic proteins determines the suseptibility of a cell to various apoptotic stimuli. Many Bcl-2 family proteins are differentially expressed in various malignancies and some are useful prognostic biomarkers. Prosurvival proteins are often elevated in diverse cancers and have the potential to confer resistance to both endogenous cell death stimuli and cancer treatments. Alterations in the ratio or levels of Bcl-2 family proteins have been also associated with nonmalignant diseases including neurodegenerative diseases, autoimmune diseases, AIDs, Down's syndrome, cardiovascular diseases, diabetes, glomerulonephritis, and muscular dystrophy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Bcl-2 Antibody (NB100-56101)(12)

Publications using NB100-56101

• Polat ME, Sar? E, Tanriverdi LH et al. A potential hepatoprotective effect of ranolazine against methotrexate-induced liver injury in rats Authorea 2023-01-01 (IHC, Rat)
• Wu Z, Hu J, Li Y et al. Assessment of renal pathophysiological processes and protective effect of quercetin on contrast-induced acute kidney injury in type 1 diabetic mice using diffusion tensor imaging Redox Report : Communications in Free Radical Research 2024-09-16 [PMID: 39284588]
• Debarpan Mitra, Depanwita Saha, Gaurav Das, Rimi Mukherjee, Samir Banerjee, Neyaz Alam, Saunak Mitra Mustafi, Partha Nath, Anuj Majumder, Biswanath Majumder, Nabendu Murmu Lupeol synergizes with 5-fluorouracil to combat c-MET/EphA2 mediated chemoresistance in triple negative breast cancer iScience 2023-11-04 [PMID: 38047085]
• Arman T, Baron J, Lynch K Et al. MCLR-elicited hepatic fibrosis and carcinogenic gene expression changes persist in rats with diet-induced nonalcoholic steatohepatitis through a 4-week recovery period Toxicology 2021-11-01 [PMID: 34740672] (WB, Rat)
• Gao S, Zhou Q, Jin H et al. Effect of pyrroloquinoline quinone on lipopolysaccharide-induced autophagy in HAPI microglia cells Annals of Translational Medicine 2021-09-01 [PMID: 34733929] (Western Blot, Immunohistochemistry-Paraffin, Canine, Mouse, Rat)
• Zhou Q, Jin H, Shi N et al. Inhibit inflammation and apoptosis of pyrroloquinoline on spinal cord injury in rat Annals of Translational Medicine 2021-09-01 [PMID: 34733912] (Western Blot, Immunohistochemistry-Paraffin, Canine, Mouse, Rat)
• Arman T, Lynch KD, Goedken M, Clarke JD Sub-chronic microcystin-LR renal toxicity in rats fed a high fat/high cholesterol diet Chemosphere 2020-10-27 [PMID: 33143886] (IHC-P, Rat)
• Shoma Ishikawa, Fuyuki Ishikawa Proteostasis failure and cellular senescence in long‐term cultured postmitotic rat neurons Aging Cell 2019-11-25 [PMID: 31762159]
• Sulaiman D, Li J, Devarajan A et al. Paraoxonase 2 protects against acute myocardial ischemia-reperfusion injury by modulating mitochondrial function and oxidative stress via the PI3K/Akt/GSK-3b RISK pathway J. Mol. Cell. Cardiol. 2019-02-23 [PMID: 30802459] (IHC-P, Mouse)
• Samdanci ET, Huz M, Ozhan O et al. Cytoprotective effects of molsidomine against methotrexate-induced hepatotoxicity: an experimental rat study Drug Des Devel Ther 2019-12-20 [PMID: 30587924] (IF/IHC, Rat)
• Rajamani U, Essop MF. Hyperglycemia-mediated activation of the hexosamine biosynthetic pathway results in myocardial apoptosis. Am J Physiol Cell Physiol. 2010-07-01 [PMID: 20410435]
• Krajewska M, Rosenthal RE, Mikolajczyk J et al. Early processing of Bid and caspase-6, -8, -10, -14 in the canine brain during cardiac arrest and resuscitation. Exp Neurol. 2004-10-01 [PMID: 15380478] (IHC-P, WB, Mouse, Canine, Rat)
  
  Details:
  Antibodies cited: 1. Caspase-9 (Active/Cleaved), IMG-5705: WB: Fig 1A (recombinant human caspase-9), Fig 4A (recombinant human caspase-9, rat brain), Fig 4B-F (isolated mitochondria from rat liver or heart). Fig 5 (PC12 cells) IHC (P): Fig 2d-k (rat kidne

Reviews for Bcl-2 Antibody (NB100-56101) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Rat.

reviewed by: Yuanyuan Tie WB Rat 11/23/2017

Summary

• Application: Western Blot
• Sample Tested: H9c2 whole cell lysate
• Species: Rat
• Lot: AR09-092206B-05

reviewed by: Verified Customer WB Human 10/17/2014

Summary

• Application: Western Blot
• Sample Tested: Whole cell lysate from 293T cells, in lysis buffer
• Species: Human

Bioinformatics

• Gene Symbol: BCL2
• Entrez:
  • 12043(Mouse)
  • 24224(Rat)