beta-Arrestin 1 Antibody (E274)

Product Discontinued

beta-Arrestin 1 Antibody (E274) Summary

• Immunogen: A synthetic peptide corresponding to residues near N-terminus of human -arrestin-1 was used as immunogen.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ARRB1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:40
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
  • Western Blot 1:1000-10000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  beta-Arrestin 1 Overexpression Lysate

Reactivity Notes

Predicted to work with: Bovine

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Protein A or G purified

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for beta-Arrestin 1 Antibody (E274)

• ARB1
• ARR1
• ARRB1
• arrestin 2
• Arrestin beta-1
• arrestin, beta 1
• betaArrestin 1
• beta-Arrestin 1
• beta-arrestin-1

Background

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization ofG-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones,neurotransmitters, or sensory signals. Arrestin beta 1 is a cytosolic protein and acts as a cofactor in thebeta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Besides the centralnervous system, it is expressed at high levels in peripheral blood leukocytes, and thus the BARK/beta-arrestin systemis believed to play a major role in regulating receptor-mediated immune functions. Alternatively spliced transcriptsencoding different isoforms of arrestin beta 1 have been described, however, their exact functions are not known.(provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta-Arrestin 1 Antibody (NB110-55485)(3)

Publications using NB110-55485

• Kumar R, Samal SK, Routray S et al. Identification of oral cancer related candidate genes by integrating protein-protein interactions, gene ontology, pathway analysis and immunohistochemistry. Sci Rep 2017-05-30 [PMID: 28559546] (IF/IHC, Human)
• Oakley RH, Revollo J, Cidlowski JA. Glucocorticoids regulate arrestin gene expression and redirect the signaling profile of G protein-coupled receptors Proc Natl Acad Sci U S A 2012-10-08 [PMID: 23045642] (WB, ICC/IF, Human)
• Wang WC, Schillinger RM, Malone MM, Liggett S. Paradoxical attenuation of B2-AR function in airway smooth muscle by Gi-mediated counterregulation in transgenic mice overexpressing type 5 adenylyl cyclase Am J Physiol Lung Cell Mol Physiol 2011-03-01 [PMID: 21131397] (WB, Mouse)

Bioinformatics

• Gene Symbol: ARRB1
• Entrez:
  • 408(Human)
  • 109689(Mouse)
  • 25387(Rat)
• Uniprot:
  • P49407()