beta-Catenin Antibody

Western Blot: beta-Catenin Antibody [NBP2-29476] - Western Blot of beta-Catenin (p120) in human brain 1) absence and 2) presence of immunizing peptide and 3) Mouse brain lysate using beta-Catenin PAb.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP2-29476] - Immunohistochemistry-Paraffin: [NBP2-29476] - Formalin-fixed, paraffin-embedded human breast ductal carcinoma stained with beta- Catenin antibody (1:100), peroxidase-conjugate and Warp Red chromogen (20X). Note specific membrane staining in ductal carcinoma.

Flow Cytometry: beta-Catenin Antibody [NBP2-29476] - Flow Cytometric Analysis of PFA fixed HeLa cells using beta-Catenin PAb followed by goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP2-29476] - Formalin-fixed, paraffin-embedded human breast lobular carcinoma stained with beta- Catenin antibody (1:100), peroxidase-conjugate and Warp Red chromogen (20X). Note specific cytoplasmic staining in lobular carcinoma.

• Reactivity: Hu, Mu
• Applications: WB, Flow, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

beta-Catenin Antibody Summary

• Description: 200ug/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP2-33124)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: A synthetic peptide from the middle of beta-Catenin protein (Uniprot: P35222)
• Localization: Cell surface and cytoplasmic
• Marker: Epithelial Cell Marker, Adherens Junction Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CTNNB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5-1 ug/million cells
  • Immunohistochemistry 1-2ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 92 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

The amino acid sequence used as the immunogen for this beta Catenin antibody is 100% homologous in a broad range of species.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A purified

Alternate Names for beta-Catenin Antibody

• bCatenin
• beta 1 (88kD)
• beta-Catenin
• catenin (cadherin-associated protein), beta 1, 88kDa
• catenin beta-1
• CTNNB
• CTNNB1
• DKFZp686D02253
• FLJ25606
• FLJ37923

Background

Beta Catenin, an adherens junction protein, was originally identified as a component of cell-cell adhesion structures. It interacts with the cytoplasmic domain of E-cadherin and links E-cadherin to alpha-catenin, which in turn mediates anchorage of the E-cadherin complex to the cortical actin cytoskeleton. Research shows that beta Catenin also binds to another cytoskeletal complex containing the adenomatous polyposis coli protein and microtubules, and interacts with several signaling pathways that include tyrosine kinases and phosphatases and Wnt/Wingless. Interplay between these cytoskeletal complexes and signaling pathways may regulate morphogenesis. Beta Catenin is expressed in several hair follicle cell types, basal and peripheral matrix cells, and cells of the outer and inner root sheats. A pathological role of beta Catenin has been identified in pilomatrixoma, medulloblastoma and tumor development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CTNNB1
• Uniprot:
  • P35222()
  • P35222()
  • P35222()