beta II Tubulin A Antibody (TUB 2.1)

Western Blot: beta II Tubulin A Antibody (TUB 2.1) [NB120-11308] - Monoclonal Anti-b-Tubulin antibody produced in mouse.

Immunocytochemistry/Immunofluorescence: beta II Tubulin A Antibody (TUB 2.1) [NB120-11308] - The reconstructed confocal image of tripolar mitotic rat hepatoma cells (FAO cells) shows spindle apparatus having three centers of mitosis (blue color). Associated chromosomes are yellow colored.

Immunocytochemistry/Immunofluorescence: beta II Tubulin A Antibody (TUB 2.1) [NB120-11308] - Colon cancer cells.

Immunocytochemistry/Immunofluorescence: beta II Tubulin A Antibody (TUB 2.1) [NB120-11308] - Confocal microscopy of rat hepatoma cell line (FAO cells) Nuclei and chromosomes are stained with propidium iodide (red). Microtubules (green) are seen in cytoplasm of non-dividing cells and in bipolar and tripolar mitotic apparatus of dividing cells.

Immunocytochemistry/Immunofluorescence: beta II Tubulin A Antibody (TUB 2.1) [NB120-11308] - Human monocytes cultured on hardware MIA in real microgravity on board International Space Station (green) and DAP for the DNA.

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beta II Tubulin A Antibody (TUB 2.1) Summary

• Immunogen: Purified rat brain tubulin.
• Localization: Major constituent of microtubules.
• Specificity: Recognizes all five isoforms of beta-tubulin. It reacts with the beta-Lc and beta-Sc fragments in the carboxy-terminal part of beta-tubulin in immunoblotting.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TUBB2A
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Simple Western
  • Western Blot 1:100-1:2000
• Application Notes: The antibody may be used to localize beta-tubulin in cultured cells or tissue sections. In WB, recognizes 55 kDa band.
  Simple Western reported by an internal validation. Separated by Charge.
• Control:

  beta II Tubulin A Overexpression Lysate

Reactivity Notes

Cross-reacts Frog, Wheat, Rabbit, Bovine, Moth, Rat, Sea Urchin. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Raw ascites
• Preservative: 0.9% Sodium Azide
• Purity: Unpurified

Alternate Names for beta II Tubulin A Antibody (TUB 2.1)

• beta II Tubulin A
• dJ40E16.7
• TUBB
• TUBB2
• TUBB2A
• TUBB2B
• tubulin beta-2A chain
• tubulin, beta 2
• tubulin, beta 2A
• tubulin, beta polypeptide 2
• tubulin, beta polypeptide

Background

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TUBB2A
• Entrez:
  • 7280(Human)
  • 22151(Mouse)
  • 498736(Rat)
• Uniprot:
  • P85108(Rat)