beta Tubulin Antibody (TUBB/4470)

Formalin-fixed, paraffin-embedded prostate stained with beta Tubulin antibody (TUBB/4470).

• Reactivity: Hu
• Applications: IHC
• Clone: TUBB/4470
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

beta Tubulin Antibody (TUBB/4470) Summary

• Description: Positive Controls: Human brain and spleen.
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
• Immunogen: Recombinant full-length human beta Tubulin protein (TUBB5)
• Localization: Cytoplasm
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TUBB
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for beta Tubulin Antibody (TUBB/4470)

• beta polypeptide
• beta Tubulin
• MGC117247
• OK/SW-cl.56
• TUBB
• TUBB5
• tubulin beta chain
• tubulin beta polypeptide
• tubulin beta-1 chain
• Tubulin beta-5 chain
• tubulin, beta

Background

Beta Tubulin antibodies are useful as loading controls for Western Blotting and ELISA, as well as in studies invlolving cell structure and division. Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TUBB