BLMH/Bleomycin Hydrolase Antibody - BSA Free

Western Blot: BLMH/Bleomycin Hydrolase Antibody [NBP1-88640] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

Immunohistochemistry-Paraffin: BLMH/Bleomycin Hydrolase Antibody [NBP1-88640] - Staining of human testis.

Western Blot: BLMH/Bleomycin Hydrolase Antibody [NBP1-88640] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Immunohistochemistry-Paraffin: BLMH/Bleomycin Hydrolase Antibody [NBP1-88640] - Staining of human liver shows low expression as expected.

Immunohistochemistry-Paraffin: BLMH/Bleomycin Hydrolase Antibody [NBP1-88640] - Staining of human skin shows high expression.

Immunohistochemistry-Paraffin: BLMH/Bleomycin Hydrolase Antibody [NBP1-88640] - Staining in human skin and liver tissues using anti-BLMH antibody. Corresponding BLMH RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: BLMH/Bleomycin Hydrolase Antibody [NBP1-88640] - Staining of human kidney, liver, skin and testis using Anti-BLMH antibody NBP1-88640 (A) shows similar protein distribution across tissues to independent antibody NBP2-39023 (B).

Immunohistochemistry-Paraffin: BLMH/Bleomycin Hydrolase Antibody [NBP1-88640] - Staining of human kidney.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

BLMH/Bleomycin Hydrolase Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: RKEPEDGRLVQFLLMNPANDGGQWDMLVNIVEKYGVIPKKCFPESYTTEATRRMNDILNHKMREFCIRLRNLVHSGATKGEISATQDVMMEEIFRVVCICLGNP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BLMH
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  BLMH/Bleomycin Hydrolase Protein (NBP1-88640PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for BLMH/Bleomycin Hydrolase Antibody - BSA Free

• BHBMHBLM hydrolase
• bleomycin hydrolase
• BLMH
• BMH
• EC 3.4.22.40

Background

Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BLMH