BNIP3L Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: BNIP3L Antibody [NBP1-78264] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-BNIP3L (NBP1-78264) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry: BNIP3L Antibody [NBP1-78264] - Analysis of BNIP3L in mouse prostate using DAB with hematoxylin counterstain.

• Reactivity: Hu, Mu, Bv
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.07 mg/ml

BNIP3L Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to an internal portion of the human BNIP3L protein (between residues 50-100) [UniProt O60238]
• Localization: Nucleus envelope. Endoplasmic reticulum. Mitochondrion. Membrane; Single-pass membrane protein.
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BNIP3L
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:300
  • Immunohistochemistry-Paraffin 1:300
• Application Notes: This BNIP3L antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry-Paraffin. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reactivity Notes

Immunogen has 100% identity to bovine.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.07 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BNIP3L Antibody - BSA Free

• Adenovirus E1B19K-binding protein B5
• BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A
• BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
• BCL2/adenovirus E1B 19-kd protein-interacting protein 3a
• BCL2/adenovirus E1B 19kDa interacting protein 3-like
• BNIP3a
• BNIP3H
• BNIP3L
• Nip3L
• NIP3-like protein X
• Nix
• NIXBCL2/adenovirus E1B 19kD-interacting protein 3-like

Background

BNIP3L/NIX is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family, which is involved in the regulation of apoptosis. Like other BH3-only proteins, NIX is believed to possess tumor suppressing functions; NIX localizes to the mitochondria and interacts with BCL2 and BCL-X in a pro-apoptotic manner. NIX shares a high level of sequence homology with BNIP3, and like BNIP3 activates apoptosis through its C-terminal transmembrane domain but not via its BH3 domain. Hypoxia is believed to be a principle regulator of NIX activity, making NIX a key factor in hypoxia-induced tumor cell death and heart disease. In addition, NIX has been implicated in mitophagy, a specific form of autophagy; due to its dual role, NIX is an interesting target to examine the relationship between apoptosis and autophagy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BNIP3L
• Entrez:
  • 534615(Bovine)
  • 665(Human)
  • 12177(Mouse)
• Uniprot:
  • O60238()