Brachyury Antibody [Allophycocyanin]

D3 mouse embryonic stem cell line differentiated with mouse serum for 4 days was stained with Goat Anti-Human/Mouse Brachyury APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2085A, filled histogram) or isotype control antibody (IC108A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

IPSC mesoderm differentiation cells treated with IPSC mesoderm differentiation (SC030B) for 2 days were stained with Goat Anti-Human/Mouse Brachyury APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2085A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). To facilitate intracellular staining, cells were fixed with FC004 and permeabilized with FoxP3 Perm. View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu, Mu
• Applications: Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Allophycocyanin

Brachyury Antibody [Allophycocyanin] Summary

• Immunogen: E. coli-derived recombinant human Brachyury
  Ser2-Glu202
  Accession # O15178
• Specificity: Detects human Brachyury in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) TBX-6 is observed, and approximately 5% cross-reactivity with rhTBX-2, rhTBX-5, and rhTBX-18 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: T
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Brachyury Antibody [Allophycocyanin]

• brachyury protein
• Brachyury
• MGC104817
• Protein T
• SAVA
• T brachyury (mouse) homolog
• T brachyury homolog
• T Brachyury Transcription Factor
• T, brachyury homolog (mouse)
• T-Box Transcription Factor T
• TBXT
• TF
• TFT

Background

Brachyury is the founding member of the T-box family of transcription factors, which is characterized by the N-terminal conserved DNA-binding T-domain. Brachyury is required in the early determination and differentiation of mesoderms. Human brachyury molecule shares 90% homology with mouse brachyury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Brachyury Antibody (IC2085A)(10)

Publications using IC2085A

• Y Fan, J Hackland, A Baggiolini, LY Hung, H Zhao, P Zumbo, P Oberst, AP Minotti, E Hergenrede, S Najjar, Z Huang, NM Cruz, A Zhong, M Sidharta, T Zhou, E de Stanchi, D Betel, RM White, M Gershon, KG Margolis, L Studer hPSC-derived sacral neural crest enables rescue in a severe model of Hirschsprung's disease Cell Stem Cell, 2023;30(3):264-282.e9. 2023 [PMID: 36868194] (Flow Cytometry, Human)
• Y Fan, J Hackland, A Baggiolini, LY Hung, H Zhao, P Zumbo, P Oberst, AP Minotti, E Hergenrede, S Najjar, Z Huang, NM Cruz, A Zhong, M Sidharta, T Zhou, E de Stanchi, D Betel, RM White, M Gershon, KG Margolis, L Studer hPSC-derived sacral neural crest enables rescue in a severe model of Hirschsprung's disease Cell Stem Cell, 2023-03-02;30(3):264-282.e9. 2023-03-02 [PMID: 36868194] (Flow Cytometry, Human, Mouse)
• D Huang, J Li, F Hu, C Xia, Q Weng, T Wang, H Peng, B Wu, H Wu, J Xiong, Y Lin, Y Wang, Q Zhang, X Liu, L Liu, X Zheng, Y Geng, X Du, X Zhu, L Wang, J Hao, J Wang Lateral plate mesoderm cell-based organoid system for NK cell regeneration from human pluripotent stem cells Cell Discovery, 2022-11-08;8(1):121. 2022-11-08 [PMID: 36344493] (Flow Cytometry, Human)
• I Fagerlund, A Dougalis, A Shakirzyan, M Gómez-Budi, A Pelkonen, H Konttinen, S Ohtonen, MF Fazaludeen, M Koskuvi, J Kuusisto, D Hernández, A Pebay, J Koistinaho, T Rauramaa, Š Lehtonen, P Korhonen, T Malm Microglia-like Cells Promote Neuronal Functions in Cerebral Organoids Cells, 2021-12-30;11(1):. 2021-12-30 [PMID: 35011686] (ICC, Human)
• Meritxell Sáez, Robert Blassberg, Elena Camacho-Aguilar, Eric D. Siggia, David A. Rand, James Briscoe Statistically derived geometrical landscapes capture principles of decision-making dynamics during cell fate transitions Cell Systems 1/19/2022 [PMID: 34536382]
• Martha E. Diaz‐Hernandez, Nazir M. Khan, Camila M. Trochez, Tim Yoon, Peter Maye, Steven M. Presciutti et al. Derivation of notochordal cells from human embryonic stem cells reveals unique regulatory networks by single cell‐transcriptomics Journal of Cellular Physiology 6/1/2020 [PMID: 31840817]
• Li-Fang Chu, Daniel Mamott, Zijian Ni, Rhonda Bacher, Cathy Liu, Scott Swanson et al. An In Vitro Human Segmentation Clock Model Derived from Embryonic Stem Cells Cell Reports 8/27/2019 [PMID: 31461642]
• Garreta E, Prado P, Tarantino C et al. Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells Nature Materials 4/1/2019 [PMID: 30778227]
• Garreta E, Prado P, Tarantino C et al. Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells Nature Materials 2019-04-01 [PMID: 30778227]
• KG Wong, SD Ryan, K Ramnarine, SA Rosen, SE Mann, A Kulick, E De Stanchi, FJ Müller, TJ Kacmarczyk, C Zhang, D Betel, MJ Tomishima CryoPause: A New Method to Immediately Initiate Experiments after Cryopreservation of Pluripotent Stem Cells Stem Cell Reports, 2017-06-08;0(0):. 2017-06-08 [PMID: 28602613] (ICC, Human)

Bioinformatics

• Gene Symbol: T
• Uniprot:
  • O15178()