PD-ECGF/Thymidine Phosphorylase Antibody (PGF 44C) - BSA Free

Immunohistochemistry: PD-ECGF/Thymidine Phosphorylase Antibody (PGF 44C) [NB100-2737] - Immunohistochemical staining of thymidine phosphorylase (TP) expression and related markers in benign lymph node. In benign lymph node, TP expression highlights the meshwork of macrophages (arrow in inset; 400x) and endothelial cells (arrowhead in inset; 400x). Images are from an Olympus BX41 microscope (Olympus Corp., Tokyo, Japan) processed with Qcapture Pro 5.1 (QImaging, Surrey, BC, Canada). Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/ah/2011/875135/) licensed under a CC-BY license.

Immunohistochemistry: PD-ECGF/Thymidine Phosphorylase Antibody (PGF 44C) [NB100-2737] - Immunohistochemical staining of thymidine phosphorylase (TP) expression and related markers in benign lymph node. In benign lymph node, TP expression highlights the meshwork of macrophages (arrow in inset; 400x) and endothelial cells (arrowhead in inset; 400x). Images are from an Olympus BX41 microscope (Olympus Corp., Tokyo, Japan) processed with Qcapture Pro 5.1 (QImaging, Surrey, BC, Canada). Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/ah/2011/875135/) licensed under a CC-BY license.

Immunohistochemistry: PD-ECGF/Thymidine Phosphorylase Antibody (PGF 44C) [NB100-2737] - Immunohistochemical staining of thymidine phosphorylase (TP) expression and related markers in benign lymph node in lymph node with malignant mycosis fungoides/Sezary syndrome cells (LN-MF). CD21 is negative in this case of LN-MF. Images are from an Olympus BX41 microscope (Olympus Corp., Tokyo, Japan) processed with Qcapture Pro 5.1 (QImaging, Surrey, BC, Canada). Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/ah/2011/875135/) licensed under a CC-BY license.

Immunohistochemistry: PD-ECGF/Thymidine Phosphorylase Antibody (PGF 44C) [NB100-2737] - Immunohistochemical staining of thymidine phosphorylase (TP) expression and related markers in benign lymph node in lymph node with malignant mycosis fungoides/Sezary syndrome cells (LN-MF). LN-MF: hematoxylin and eosin (H&E) stain. Images are from an Olympus BX41 microscope (Olympus Corp., Tokyo, Japan) processed with Qcapture Pro 5.1 (QImaging, Surrey, BC, Canada). Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/ah/2011/875135/) licensed under a CC-BY license.

Immunohistochemistry: PD-ECGF/Thymidine Phosphorylase Antibody (PGF 44C) [NB100-2737] - Immunohistochemical staining of thymidine phosphorylase (TP) expression and related markers in benign lymph node in lymph node with malignant mycosis fungoides/Sezary syndrome cells (LN-MF). CD3 highlights the malignant T cells and reactive T cells in LN-MF. Images are from an Olympus BX41 microscope (Olympus Corp., Tokyo, Japan) processed with Qcapture Pro 5.1 (QImaging, Surrey, BC, Canada). Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/ah/2011/875135/) licensed under a CC-BY license.

Immunohistochemistry: PD-ECGF/Thymidine Phosphorylase Antibody (PGF 44C) [NB100-2737] - Immunohistochemical staining of thymidine phosphorylase (TP) expression and related markers in benign lymph node in lymph node with malignant mycosis fungoides/Sezary syndrome cells (LN-MF). CD4 is weakly expressed in the malignant T cells in LN-MF. Images are from an Olympus BX41 microscope (Olympus Corp., Tokyo, Japan) processed with Qcapture Pro 5.1 (QImaging, Surrey, BC, Canada). Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/ah/2011/875135/) licensed under a CC-BY license.

Immunohistochemistry: PD-ECGF/Thymidine Phosphorylase Antibody (PGF 44C) [NB100-2737] - Immunohistochemical staining of thymidine phosphorylase (TP) expression and related markers in benign lymph node in lymph node with malignant mycosis fungoides/Sezary syndrome cells (LN-MF). CD68 highlights the macrophages in LN-MF. Images are from an Olympus BX41 microscope (Olympus Corp., Tokyo, Japan) processed with Qcapture Pro 5.1 (QImaging, Surrey, BC, Canada). Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/ah/2011/875135/) licensed under a CC-BY license.

Immunohistochemistry: PD-ECGF/Thymidine Phosphorylase Antibody (PGF 44C) [NB100-2737] - Immunohistochemical staining of thymidine phosphorylase (TP) expression and related markers in benign lymph node in lymph node with malignant mycosis fungoides/Sezary syndrome cells (LN-MF). TP highlights the macrophages (cytoplasmic/nuclear pattern, arrowhead in inset 1; 400x) and the malignant T cells (cytoplasmic pattern, arrow in inset 2; 400x) in a cytoplasmic staining pattern. Multiple mitotic figures are also noted in the malignant T cells. Images are from an Olympus BX41 microscope (Olympus Corp., Tokyo, Japan) processed with Qcapture Pro 5.1 (QImaging, Surrey, BC, Canada). Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/ah/2011/875135/) licensed under a CC-BY license.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clone: PGF 44C
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PD-ECGF/Thymidine Phosphorylase Antibody (PGF 44C) - BSA Free Summary

• Immunogen: Full length human protein in E. coli
• Localization: Cytoplasmic and nuclear.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TYMP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100-1:2000
• Application Notes: PGF 44C blocks enzyme activity. Positive control(s): HUVEC cell or breast carcinoma.
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PD-ECGF/Thymidine Phosphorylase Antibody (PGF 44C) - BSA Free

• dThdPase
• EC 2.4.2
• EC 2.4.2.4
• ECGF
• ECGF1
• ECGF1TP
• endothelial cell growth factor 1 (platelet-derived)
• Gliostatin
• hPD-ECGF
• MEDPS1
• MNGIE
• MTDPS1
• PDECGF
• PD-ECGF
• Platelet-derived endothelial cell growth factor
• TdRPase
• Thymidine Phosphorylase
• TPase
• TYMP

Background

Platelet - derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyses the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PD-ECGF/Thymidine Phosphorylase Antibody (NB100-2737)(1)

Publication using NB100-2737

• Nie X, Bhat R, Al-Saleem ED et al. Expression of Thymidine Phosphorylase in Lymph Nodes Involved with Mycosis Fungoides and Sezary Syndrome. Advances in Hematology. 2011-01-01 [PMID: 22162690]

Bioinformatics

• Gene Symbol: TYMP
• Entrez:
  • 1890(Human)
  • 72962(Mouse)
  • 315219(Rat)