BRF2 Antibody - BSA Free

Western Blot: BRF2 Antibody [NBP2-15617] - Sample (30 ug of whole cell lysate) A: A549 B: Hela C: HCT116 10% SDS PAGE gel, diluted at 1:1000.

Western Blot: BRF2 Antibody [NBP2-15617] - Sample (30 ug of whole cell lysate) A: A549 B: Hela C: HCT116 10% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: BRF2 Antibody [NBP2-15617] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: BRF2 Antibody [NBP2-15617] - Ca922 xenograft, using BRF2 antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

• Reactivity: Hu, Rt, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

BRF2 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human BRF2. The exact sequence is proprietary.
• Predicted Species: Rat (90%), Bovine (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BRF2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for BRF2 Antibody - BSA Free

• B-related factor 2
• BRF-2
• BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like
• BRFUhTFIIIB50
• FLJ11052
• hBRFU
• TFIIIB50RNA polymerase III transcription initiation factor BRFU
• transcription factor IIB- related factor, TFIIIB50
• transcription factor IIIB 50 kDa subunit

Background

This gene encodes one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. The product of this gene, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: BRF2
• Uniprot:
  • Q9HAW0(Human)