C-Reactive Protein/CRP Antibody [Unconjugated]

Western blot shows lysates of human kidney tissue . PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human C-Reactive Protein/CRP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1707) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for C-Reactive Protein/CRP at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

C-Reactive Protein/CRP was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using 1.7 µg/mL Human C-Reactive Protein/CRP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1707) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to epithelial cells in tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

C-Reactive Protein/CRP was detected in immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes using Sheep Anti-Human C-Reactive Protein/CRP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1707) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Simple Western lane view shows lysates of Human Plasma, Human Liver, loaded at 0.2 mg/mL. A specific band was detected for C‑Reactive Protein/CRP at approximately 29 kDa (as indicated) using 5 µg/mL of Sheep Anti-Human C‑Reactive Protein/CRP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1707) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

C-Reactive Protein/CRP Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human C‑Reactive Protein/CRP
  Phe17-Pro224
  Accession # P02741
• Specificity: Detects human C‑Reactive Protein/CRP in direct ELISAs and Western blots. In Western blots, approximately 35% cross‑reactivity with recombinant mouse CRP and 10% cross-reactivity with recombinant rat CRP is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CRP
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for C-Reactive Protein/CRP Antibody [Unconjugated]

• C-Reactive Protein
• C-reactive protein, pentraxin-related
• CRP
• MGC88244
• pentraxin 1
• PTX1MGC149895

Background

CRP is a member of the pentraxin family of proteins that are characterized by a cyclic pentameric structure. Human CRP gene encodes a 224 amino acids precursor. The mature human CRP protein has 206 amino acids that are non-covalently linked to form the pentameter. Human CRP shares 71% and 64% amino acid sequence homology with mouse and rat respectively.

CRP, synthesized by hepatocytes, is a major acute phase serum protein in human. IL-6, IL-1 and glucocorticoids are the major inducer of the CRP gene. In response to infection, inflammation or tissue damage, the level of CRP in human serum can increase 1,000-fold within 24‑48 hours. It will come back to base level of less than 1 μg/mL very fast. Human CRP is an acute-phase serum protein that plays a role in the first line in host innate host defense. Like other pentraxins, CRP exhibits Ca⁺⁺ - dependent binding to ligands. Phosphocholine (PCh), a constituent of many bacterial and fungal walls, is a principal ligand of CRP. CRP also binds to the membrane of injured cells, membrane and nuclear components of necrotic and apoptotic cells. Upon binding with the ligands, CRP is recognized by C1q and initiates the activation of complement cascade. Ligand bound CRP also binds to Fc gamma RI and Fc gamma RIIa on phagocytes and activates phogocytotic responses. In addition to phogocytosis, CRP also can induce production of hydrogen peroxide and inflammatory cytokines, such as IL-1, IL-6 and TNF‑ alpha  by monocytes. With these functions, human CRP is an important serum protein for anti-bacterial pathogen and clearance of damaged and apoptotic cells. However, in mouse, CRP is expressed at very low level and is not an acute phase reactant. Serum amyloid P component (SAP), another pentraxin, is the major acute phase serum protein in mice. It has been shown that high levels of CRP in humans is associated with an increased risk of cardiovascular diseases.

1. Gotschlich, E.C. and G.M. Edelman (1965) Proc. Natl. Acad. Sci. USA 54:558.
2. Volanakis, J.E. (2001) Molecular Immunology 38:189.
3. Bharadwaj, D. et al. (1999) J. Experimental Medicine 190:585.
4. Ballou S.P. and G. Lozanski (1992) Cytokine 4:361.
5. Danesh, J. et al. (2004) N. Engl. J. Med. 350:1387.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for C-Reactive Protein/CRP Antibody (AF1707)(2)

Publications using AF1707

• Newman, S;Wilson, B;Mamerow, D;Wollant, B;Nyein, H;Rosenberg-Hasson, Y;Maecker, H;Eisenstein, M;Soh, H; Extending the dynamic range of biomarker quantification through molecular equalization bioRxiv 2023-01-01 [PMID: 37443317] (Bioassay, Human)
• Lijie Zhao, Tingli Sun, Lina Wang Chitosan oligosaccharide improves the therapeutic efficacy of sitagliptin for the therapy of Chinese elderly patients with type 2 diabetes mellitus Therapeutics and Clinical Risk Management 7/20/2017 [PMID: 28721055]

Bioinformatics

• Gene Symbol: CRP
• Uniprot:
  • P02741()