Procalcitonin Antibody (44D9) - BSA Free

• Reactivity: Hu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 44D9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Procalcitonin Antibody (44D9) - BSA Free Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with fragments of human Procalcitonin conjugated with carrier protein.
• Localization: Secreted
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CALCA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Sandwich ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: Recommended pairs for sandwich immunoassay (capture-detection): NBP1-79064 - NB120-14817.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Procalcitonin Antibody (44D9) - BSA Free

• CALC1
• CALCA
• Calcitonin 1
• calcitonin gene-related peptide 1
• CGRP
• Katacalcin
• Procalcitonin

Background

Procalcitonin (PCT) is a 116 amino acid residue peptide with molecular weight of about 13 kDa. PCT itself has no known hormonal activity. PCT belongs to a group of related proteins including calcitonin gene-related peptides I and II, amylin, adrenomodulin and calcitonin (CAPA peptide family). PCT, like other peptides of CAPA family, appears from the common precursor pre-procalcitonin consisting of 141 amino acids by removal of 25 amino acids from the N-terminus. PCT undergoes successive cleavages to form three molecules: N-terminal fragment (55 a.a.), calcitonin (32 a.a.) and katacalcin (21 a.a.). Under normal metabolic conditions, PCT is only present in the C cells of the thyroid gland. In bacterial infection and sepsis, however, intact PCT is found in the blood and, more importantly, its level is related to the severity of bacterial sepsis. Today, PCT is considered to be one of the earliest and most specific markers of sepsis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Procalcitonin Antibody (NB120-14817)(10)

Publications using NB120-14817

• Tavares E, Maldonado R, Garcia-Martinez A, Minano FJ. Central Administration of Aminoprocalcitonin Inhibits Food Intake and Stimulates the Hypothalamic-Pituitary-Adrenal Axis in Rats via the Corticotrophin-Releasing Factor System J Neuroendocrinol 2012-07-01 [PMID: 22372932] (WB, IF/IHC, ICC/IF, Rat)
• Selimo?lu F, Ayhan M Silver nanoparticle decorated graphene-based SERS electrode towards procalcitonin detection Vibrational Spectroscopy 2023-05-01
• Song C, Zhao J, Hao J et al. Aminoprocalcitonin protects against hippocampal neuronal death via preserving oxidative phosphorylation in refractory status epilepticus Cell death discovery 2023-05-04 [PMID: 37142587] (WB, IHC-Fr, EM, Rat)
  
  Details:
  dilutions: EM: 1:100, IHC-Fr 1:100; WB 1:500
• Ang SX, Chen CP, Sun FJ, Chen CY Bio-Functionalized Magnetic Nanoparticles for the Immunoassay of C-Reactive Protein and Procalcitonin in Cervicovaginal Secretions of Pregnant Women with Preterm Prelabor Rupture of Membranes to Predict Early-Onset Neonatal Sepsis International journal of nanomedicine 2022-01-20 [PMID: 35082495]
• Ma S, Wang H, Li W Et al. The correlation between the expression of ATF4 and procalcitonin combined with the detection of RET mutation and the pathological stage and clinical prognosis of medullary thyroid carcinoma Canadian journal of physiology and pharmacology 2021-11-25 [PMID: 34822305]
• Wang W, Xu L, Hu D, Tang W Case of an abnormal procalcitonin producing metastatic pancreatic neuroendocrine tumor Clin Case Rep 2020-11-25 [PMID: 33235774]
• Hui-Heng L, Jun-Sheng W, Jin-Zhou L et al. Lupeol Counteracts the Proinflammatory Signalling Triggered in Macrophages by 7-Keto-Cholesterol: New Perspectives in the Therapy of Atherosclerosis Cytokine 2020-09-27 [PMID: 33039977] (WB, Rat)
• Takahashi K, Ozawa E et al. Hepatobiliary and Pancreatic: A procalcitonin-secreting and calcitonin-secreting pancreatic neuroendocrine carcinoma. J Gastroenterol Hepatol 2019-01-06 [PMID: 30663800] (IF/IHC, Human)
• Luohai Chen, Yu Zhang, Yuan Lin et al. The role of elevated serum procalcitonin in neuroendocrine neoplasms of digestive system. Clinical Biochemistry 2017-06-26 [PMID: 28668469] (IF/IHC, Human)
• Tavares E, Maldonado R, Minano FJ. Immunoneutralization of Endogenous Aminoprocalcitonin Attenuates Sepsis-Induced Acute Lung Injury and Mortality in Rats. Am. J. Pathol. 2014-09-20 [PMID: 25251058]
  
  Details:
  Procalcitonin/ aminoprocalcitonin or NPCT antibody used for WB at 1:2000 dilution on Rat's lung tissues - Figure 1B shows a specific 8kDa band representing Rat's NPCT

Review for Procalcitonin Antibody (NB120-14817) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 07/15/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Cancer Tissue
• Species: Human

Comments

• Comments: The dilution of 1:300 is applicable for immunohistochemistry.

FAQs for Procalcitonin Antibody (NB120-14817). (Showing 1 - 1 of 1 FAQs).

1. One of our customers wants to buy PCT antibodies for ELISA detection from Novus and he has searched all the PCT antibodies on Novus website and has some questions as follows: 1. NB110-16925 (On the Novus website, NBP1-79065 is recommended for coating and NB110-16925 is for Conjugate. I want to know whether you have done some validation about your suggestion to confirm this recommended pair for sandwich immunoassay is the best choice. If so, I will not try other optimization such as coating plate with NB110-16925 and using NBP1-79065 for conjugate so as not to waste time.) 2. NB120-14817 (My question is the same as NB110-16925 and items below is the recommendation from Novus website: Recommended pairs for sandwich immunoassay (coating - conjugate): NBP1-79064(Coating) - NB120-14817(Conjugate)). 3. NB110-13044 (Do you have any recommendation pairs for this antibody? There is no suggestion about sandwich immunoassay on Novus website.)
   • To answer your questions the antibodies that are recommended as a pair are in fact ones that have been tested together and shown as effective sandwich pairs. We will of course always guarantee all stated species and applications, but the ones that have indications to be used together are the ones we recommend for the customers. In regards to the last antibody I am not sure what pair was used for that because I do not see it in the notes so I cannot say with 100% certainty which one would be best to use for that one and would try to stick to the others.

Bioinformatics

• Gene Symbol: CALCA
• Entrez:
  • 796(Human)
• Uniprot:
  • P06881(Human)