TFF2 Antibody (366508) [Unconjugated]

TFF2 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human TFF2 Monoclonal Antibody (Catalog # MAB4077) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 366508
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

TFF2 Antibody (366508) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human TFF2
  Glu24-Tyr129
  Accession # Q03403
• Specificity: Detects human TFF2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human TFF3.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TFF2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TFF2 Antibody (366508) [Unconjugated]

• SML1
• SML1trefoil factor 2, SML1, human spasmolytic polypeptide (SP)10spasmolytic protein 1
• SP
• Spasmolysin
• Spasmolytic Polypeptide
• TFF2
• trefoil factor 2

Background

Trefoil Factor 2 (TFF2), previously called spasmolysin, is one of three trefoil peptides secreted by epithelial cells that line mucus membranes. Trefoils contribute to protection and repair of the gastrointestinal tract. TFF2 is secreted by epithelia of the basal gastric glands and duodenal Brunner’s glands, but is also found in spleen, thymus and lung and may modulate leukocyte recruitment to sites of inflammation in these tissues. The 129 amino acid (aa) human TFF2 contains two trefoil structures formed by intramolecular disulfides and shows 82% aa identity with mouse TFF2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TFF2 Antibody (MAB4077)(2)

Publications using MAB4077

• Oliver, AJ;Huang, N;Bartolome-Casado, R;Li, R;Koplev, S;Nilsen, HR;Moy, M;Cakir, B;Polanski, K;Gudiño, V;Melón-Ardanaz, E;Sumanaweera, D;Dimitrov, D;Milchsack, LM;FitzPatrick, MEB;Provine, NM;Boccacino, JM;Dann, E;Predeus, AV;To, K;Prete, M;Chapman, JA;Masi, AC;Stephenson, E;Engelbert, J;Lobentanzer, S;Perera, S;Richardson, L;Kapuge, R;Wilbrey-Clark, A;Semprich, CI;Ellams, S;Tudor, C;Joseph, P;Garrido-Trigo, A;Corraliza, AM;Oliver, TRW;Hook, CE;James, KR;Mahbubani, KT;Saeb-Parsy, K;Zilbauer, M;Saez-Rodriguez, J;Høivik, ML;Bækkevold, ES;Stewart, CJ;Berrington, JE;Meyer, KB;Klenerman, P;Salas, A;Haniffa, M;Jahnsen, FL;Elmentaite, R;Teichmann, SA; Single-cell integration reveals metaplasia in inflammatory gut diseases Nature 2024-11-01 [PMID: 39567783] (Immunohistochemistry, Human)
• Y Kimura, K Ikuta, T Kimura, T Chiba, H Oshima, M Oshima, E Nishi, H Seno Nardilysin regulates inflammation, metaplasia, and tumors in murine stomach Sci Rep, 2017-02-23;7(0):43052. 2017-02-23 [PMID: 28230087] (IHC, Mouse)

Bioinformatics

• Gene Symbol: TFF2
• Uniprot:
  • Q03403()